Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota
ObjectiveThe primary objective of this study was to assess the impact of fecal microbiota transplantation (FMT) on serum biochemical parameters, renal injury, and gut microbiota in hyperuricemia (HUA) mice.MethodsSix-week-old male C57BL/6 J mice were given a high-purine diet and potassium oxonate in...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1599107/full |
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| author | Songjian Yuan Wenting Jia Xiaomei Liu Ruzhen Liu Man Cao Yuting Wu Yuantao Li Wei Xu Chuanxing Xiao Chuanxing Xiao Zhenqiang Hong Bangzhou Zhang Bangzhou Zhang |
| author_facet | Songjian Yuan Wenting Jia Xiaomei Liu Ruzhen Liu Man Cao Yuting Wu Yuantao Li Wei Xu Chuanxing Xiao Chuanxing Xiao Zhenqiang Hong Bangzhou Zhang Bangzhou Zhang |
| author_sort | Songjian Yuan |
| collection | DOAJ |
| description | ObjectiveThe primary objective of this study was to assess the impact of fecal microbiota transplantation (FMT) on serum biochemical parameters, renal injury, and gut microbiota in hyperuricemia (HUA) mice.MethodsSix-week-old male C57BL/6 J mice were given a high-purine diet and potassium oxonate injections to induce HUA, followed by a two-week FMT treatment. Regular body weight checks, serum biochemical analyses, and fecal sampling for 16S rRNA gene sequencing were conducted to evaluate the treatment’s impact on gut microbiota.ResultsThe model group showed significant increases in uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN) levels, and increased xanthine oxidase (XOD) activity compared to controls (p < 0.05). FMT treatment effectively reduced these levels and XOD activity (p < 0.05). At the genus level, specific taxa like Muribaculaceae and Prevotellaceae_UCG-001 were less abundant, while Blautia and Ruminiclostridium_9 were more abundant in the model group. Following FMT, gut microbiota composition returned to near-normal levels, with significant differences from the model group (p < 0.05).ConclusionThis study demonstrates that FMT holds therapeutic potential for HUA mice by reducing UA levels, alleviating renal damage, and restoring gut microbiota balance. |
| format | Article |
| id | doaj-art-e96432a156934472a0bb96f0b968173e |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-e96432a156934472a0bb96f0b968173e2025-08-20T03:59:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.15991071599107Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiotaSongjian Yuan0Wenting Jia1Xiaomei Liu2Ruzhen Liu3Man Cao4Yuting Wu5Yuantao Li6Wei Xu7Chuanxing Xiao8Chuanxing Xiao9Zhenqiang Hong10Bangzhou Zhang11Bangzhou Zhang12Fujian University of Traditional Chinese Medicine, Fuzhou, ChinaFujian University of Traditional Chinese Medicine, Fuzhou, ChinaFujian University of Traditional Chinese Medicine, Fuzhou, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaFujian University of Traditional Chinese Medicine, Fuzhou, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaFujian University of Traditional Chinese Medicine, Fuzhou, ChinaFujian University of Traditional Chinese Medicine, Fuzhou, ChinaXiamen Treatgut Biotechnology Co. Ltd., Xiamen, ChinaObjectiveThe primary objective of this study was to assess the impact of fecal microbiota transplantation (FMT) on serum biochemical parameters, renal injury, and gut microbiota in hyperuricemia (HUA) mice.MethodsSix-week-old male C57BL/6 J mice were given a high-purine diet and potassium oxonate injections to induce HUA, followed by a two-week FMT treatment. Regular body weight checks, serum biochemical analyses, and fecal sampling for 16S rRNA gene sequencing were conducted to evaluate the treatment’s impact on gut microbiota.ResultsThe model group showed significant increases in uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN) levels, and increased xanthine oxidase (XOD) activity compared to controls (p < 0.05). FMT treatment effectively reduced these levels and XOD activity (p < 0.05). At the genus level, specific taxa like Muribaculaceae and Prevotellaceae_UCG-001 were less abundant, while Blautia and Ruminiclostridium_9 were more abundant in the model group. Following FMT, gut microbiota composition returned to near-normal levels, with significant differences from the model group (p < 0.05).ConclusionThis study demonstrates that FMT holds therapeutic potential for HUA mice by reducing UA levels, alleviating renal damage, and restoring gut microbiota balance.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1599107/fullfecal microbiota transplantationhyperuricemiamicrobiomerenal injurygut microbiota |
| spellingShingle | Songjian Yuan Wenting Jia Xiaomei Liu Ruzhen Liu Man Cao Yuting Wu Yuantao Li Wei Xu Chuanxing Xiao Chuanxing Xiao Zhenqiang Hong Bangzhou Zhang Bangzhou Zhang Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota Frontiers in Microbiology fecal microbiota transplantation hyperuricemia microbiome renal injury gut microbiota |
| title | Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota |
| title_full | Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota |
| title_fullStr | Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota |
| title_full_unstemmed | Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota |
| title_short | Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota |
| title_sort | therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota |
| topic | fecal microbiota transplantation hyperuricemia microbiome renal injury gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1599107/full |
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