Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope
We recently demonstrated polarisation differential phase contrast microscopy (pDPC) as a robust, low-cost single-shot implementation of (semi)quantitative phase imaging based on differential phase microscopy. pDPC utilises a polarisation-sensitive camera to simultaneously acquire four obliquely tran...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | HardwareX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067224001160 |
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| author | Huihui Liu Sunil Kumar Edwin Garcia William Flanagan Jonathan Lightley Christopher Dunsby Paul M.W. French |
| author_facet | Huihui Liu Sunil Kumar Edwin Garcia William Flanagan Jonathan Lightley Christopher Dunsby Paul M.W. French |
| author_sort | Huihui Liu |
| collection | DOAJ |
| description | We recently demonstrated polarisation differential phase contrast microscopy (pDPC) as a robust, low-cost single-shot implementation of (semi)quantitative phase imaging based on differential phase microscopy. pDPC utilises a polarisation-sensitive camera to simultaneously acquire four obliquely transilluminated images from which phase images mapping spatial variation of optical path difference can be calculated. pDPC microscopy can be implemented on existing or bespoke microscopes and can utilise radiation at a wide range of visible to near infrared wavelengths and so is straightforward to integrate with fluorescence microscopy. Here we present a low-cost open-source pDPC module that is designed for use with the modular open-source microscope stand “openFrame”. With improved hardware and software, this new pDPC implementation provides a real-time readout of phase across a field of view that facilitates optimisation of system alignment. We also provide protocols for background subtraction and correction of crosstalk. |
| format | Article |
| id | doaj-art-e9360ca897f841c39d2931c5019af267 |
| institution | Kabale University |
| issn | 2468-0672 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj-art-e9360ca897f841c39d2931c5019af2672025-01-12T05:25:33ZengElsevierHardwareX2468-06722025-03-0121e00622Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscopeHuihui Liu0Sunil Kumar1Edwin Garcia2William Flanagan3Jonathan Lightley4Christopher Dunsby5Paul M.W. French6LIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK; Corresponding author.LIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKLIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK; Department of Surgery & Cancer, Imperial College London SW7 2AZ, UKLIGHT Community, Physics Department, Imperial College London SW7 2AZ, UKLIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKLIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKLIGHT Community, Physics Department, Imperial College London SW7 2AZ, UK; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKWe recently demonstrated polarisation differential phase contrast microscopy (pDPC) as a robust, low-cost single-shot implementation of (semi)quantitative phase imaging based on differential phase microscopy. pDPC utilises a polarisation-sensitive camera to simultaneously acquire four obliquely transilluminated images from which phase images mapping spatial variation of optical path difference can be calculated. pDPC microscopy can be implemented on existing or bespoke microscopes and can utilise radiation at a wide range of visible to near infrared wavelengths and so is straightforward to integrate with fluorescence microscopy. Here we present a low-cost open-source pDPC module that is designed for use with the modular open-source microscope stand “openFrame”. With improved hardware and software, this new pDPC implementation provides a real-time readout of phase across a field of view that facilitates optimisation of system alignment. We also provide protocols for background subtraction and correction of crosstalk.http://www.sciencedirect.com/science/article/pii/S2468067224001160Quantitative phase imagingDifferential phase contrastPolarisationMicroscopy |
| spellingShingle | Huihui Liu Sunil Kumar Edwin Garcia William Flanagan Jonathan Lightley Christopher Dunsby Paul M.W. French Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope HardwareX Quantitative phase imaging Differential phase contrast Polarisation Microscopy |
| title | Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope |
| title_full | Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope |
| title_fullStr | Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope |
| title_full_unstemmed | Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope |
| title_short | Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope |
| title_sort | open source implementation of polarisation resolved single shot differential phase contrast microscopy pdpc on a modular openframe based microscope |
| topic | Quantitative phase imaging Differential phase contrast Polarisation Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2468067224001160 |
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