Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example
Fasting is beneficial to alleviate fatty liver, lose weight and improve reproductive function. However, previous studies have shown that, during fasting, disorders of bile acid metabolism were strongly associated with intestinal inflammation. The physiological and biochemical parameters and gene exp...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124013245 |
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| author | Jun Zhang Yujie Gong Yidan Zhu Qingduo Zeng Hao Zhang Ruili Han Yujie Guo Donghua Li Yadong Tian Xiangtao Kang Yawei Yang Zhuanjian Li Ruirui Jiang |
| author_facet | Jun Zhang Yujie Gong Yidan Zhu Qingduo Zeng Hao Zhang Ruili Han Yujie Guo Donghua Li Yadong Tian Xiangtao Kang Yawei Yang Zhuanjian Li Ruirui Jiang |
| author_sort | Jun Zhang |
| collection | DOAJ |
| description | Fasting is beneficial to alleviate fatty liver, lose weight and improve reproductive function. However, previous studies have shown that, during fasting, disorders of bile acid metabolism were strongly associated with intestinal inflammation. The physiological and biochemical parameters and gene expression of multiple tissues of chickens at every critical time node were measured by ELISA and qPCR. In addition, association analysis was performed based on liver transcriptome sequencing and cecum metabolome data. At the cellular level, the regulatory effects of cecal metabolites on host bile acid metabolism were verified. During fasting, hepatic FXR-SHP-CYP7A1 and ileum-hepatic FXR-SHP-FGF15/19-FGFR4-CYP7A1 negative feedback pathways were activated to inhibit hepatic bile acid synthesis. The ileum FXR-SHP-ASBT pathways are activated, hindered the ileal bile reflux. At the same time, it promotes the secretion of bile acids and cholesterol in the liver, accelerates the utilization of H2O and CO2, to maintain liver homeostasis during fasting. In addition, enhanced gallbladder contraction and increased hunger were observed in laying hens during fasting. At the cellular level, the correlation between CYP7A1 and L-valine was verified, revealing that cecal metabolites of laying hens was enabled to regulate host bile acid metabolism. This study explored the metabolic patterns of bile acids during fasting and identified the main reasons for the accumulation of bile acids in the cecum, which provides a basis for fasting research and offers a reference for the formulation of fasting protocols. |
| format | Article |
| id | doaj-art-e8d50f58799549878e75854abb766f7d |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e8d50f58799549878e75854abb766f7d2025-01-13T04:18:17ZengElsevierPoultry Science0032-57912025-02-011042104746Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an exampleJun Zhang0Yujie Gong1Yidan Zhu2Qingduo Zeng3Hao Zhang4Ruili Han5Yujie Guo6Donghua Li7Yadong Tian8Xiangtao Kang9Yawei Yang10Zhuanjian Li11Ruirui Jiang12College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, ChinaHongyan Molting Research Institute, Xianyang 712000, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, China; Corresponding authors.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, China; Corresponding authors.Fasting is beneficial to alleviate fatty liver, lose weight and improve reproductive function. However, previous studies have shown that, during fasting, disorders of bile acid metabolism were strongly associated with intestinal inflammation. The physiological and biochemical parameters and gene expression of multiple tissues of chickens at every critical time node were measured by ELISA and qPCR. In addition, association analysis was performed based on liver transcriptome sequencing and cecum metabolome data. At the cellular level, the regulatory effects of cecal metabolites on host bile acid metabolism were verified. During fasting, hepatic FXR-SHP-CYP7A1 and ileum-hepatic FXR-SHP-FGF15/19-FGFR4-CYP7A1 negative feedback pathways were activated to inhibit hepatic bile acid synthesis. The ileum FXR-SHP-ASBT pathways are activated, hindered the ileal bile reflux. At the same time, it promotes the secretion of bile acids and cholesterol in the liver, accelerates the utilization of H2O and CO2, to maintain liver homeostasis during fasting. In addition, enhanced gallbladder contraction and increased hunger were observed in laying hens during fasting. At the cellular level, the correlation between CYP7A1 and L-valine was verified, revealing that cecal metabolites of laying hens was enabled to regulate host bile acid metabolism. This study explored the metabolic patterns of bile acids during fasting and identified the main reasons for the accumulation of bile acids in the cecum, which provides a basis for fasting research and offers a reference for the formulation of fasting protocols.http://www.sciencedirect.com/science/article/pii/S0032579124013245FastingLiverBile acidsLaying henL-valine |
| spellingShingle | Jun Zhang Yujie Gong Yidan Zhu Qingduo Zeng Hao Zhang Ruili Han Yujie Guo Donghua Li Yadong Tian Xiangtao Kang Yawei Yang Zhuanjian Li Ruirui Jiang Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example Poultry Science Fasting Liver Bile acids Laying hen L-valine |
| title | Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example |
| title_full | Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example |
| title_fullStr | Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example |
| title_full_unstemmed | Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example |
| title_short | Exploring the metabolic patterns and response mechanisms of bile acids during fasting: A study with poultry as an example |
| title_sort | exploring the metabolic patterns and response mechanisms of bile acids during fasting a study with poultry as an example |
| topic | Fasting Liver Bile acids Laying hen L-valine |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124013245 |
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