Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders
Extracellular vesicles (EVs), nanoscale vesicles released by various cell types, have garnered significant attention in regenerative medicine. Mesenchymal stem cell-derived EVs (MSC-EVs) exhibit unique advantages, including their compact size, ability to traverse the blood-brain barrier (BBB), low i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1626996/full |
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| author | Shuang Li Shuang Li Jiayi Zhang Jiayi Zhang Luyao Sun Ze Yang Xinxing Liu Jianling Liu Xifu Liu |
| author_facet | Shuang Li Shuang Li Jiayi Zhang Jiayi Zhang Luyao Sun Ze Yang Xinxing Liu Jianling Liu Xifu Liu |
| author_sort | Shuang Li |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), nanoscale vesicles released by various cell types, have garnered significant attention in regenerative medicine. Mesenchymal stem cell-derived EVs (MSC-EVs) exhibit unique advantages, including their compact size, ability to traverse the blood-brain barrier (BBB), low immunogenicity, and high biosafety profile. However, challenges such as standardization of isolation protocols, establishment of quality control criteria, and scalability of production remain unresolved. This review critically examines the methodologies for preparation, characterization, and pharmacokinetic profiling of MSC-EVs, alongside their therapeutic potential in neurological disorders. By synthesizing current advancements, this work aims to elucidate the translational value of EVs in clinical practice. Additionally, it seeks to accelerate their transition from preclinical research to therapeutic applications, and provide a robust theoretical foundation for novel strategies in treating neurological diseases. |
| format | Article |
| id | doaj-art-e8960442b74f48c79db5ac54dc6f9da6 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-e8960442b74f48c79db5ac54dc6f9da62025-08-20T03:46:45ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-08-011310.3389/fcell.2025.16269961626996Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disordersShuang Li0Shuang Li1Jiayi Zhang2Jiayi Zhang3Luyao Sun4Ze Yang5Xinxing Liu6Jianling Liu7Xifu Liu8Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Anti-Tumour Molecular Target Technology Innovation Center, College of Life Science, Hebei Normal University, Shijiazhuang, ChinaJianyuan Precision Medicines (Zhangjiakou) Co., Ltd., Zhangjiakou, ChinaJianyuan Precision Medicines (Zhangjiakou) Co., Ltd., Zhangjiakou, ChinaShijiazhuang Zhuohan Biological Technology Co., Ltd., Shijiazhuang, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Anti-Tumour Molecular Target Technology Innovation Center, College of Life Science, Hebei Normal University, Shijiazhuang, ChinaJianyuan Precision Medicines (Zhangjiakou) Co., Ltd., Zhangjiakou, ChinaJianyuan Precision Medicines (Zhangjiakou) Co., Ltd., Zhangjiakou, ChinaGuangxi Academy of Sciences, Nanning, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Anti-Tumour Molecular Target Technology Innovation Center, College of Life Science, Hebei Normal University, Shijiazhuang, ChinaExtracellular vesicles (EVs), nanoscale vesicles released by various cell types, have garnered significant attention in regenerative medicine. Mesenchymal stem cell-derived EVs (MSC-EVs) exhibit unique advantages, including their compact size, ability to traverse the blood-brain barrier (BBB), low immunogenicity, and high biosafety profile. However, challenges such as standardization of isolation protocols, establishment of quality control criteria, and scalability of production remain unresolved. This review critically examines the methodologies for preparation, characterization, and pharmacokinetic profiling of MSC-EVs, alongside their therapeutic potential in neurological disorders. By synthesizing current advancements, this work aims to elucidate the translational value of EVs in clinical practice. Additionally, it seeks to accelerate their transition from preclinical research to therapeutic applications, and provide a robust theoretical foundation for novel strategies in treating neurological diseases.https://www.frontiersin.org/articles/10.3389/fcell.2025.1626996/fullmesenchymal stem cell-derived extracellular vesicles (MSC-EVs)preparationcharacterizationpharmacokineticsneurological disorders |
| spellingShingle | Shuang Li Shuang Li Jiayi Zhang Jiayi Zhang Luyao Sun Ze Yang Xinxing Liu Jianling Liu Xifu Liu Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders Frontiers in Cell and Developmental Biology mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) preparation characterization pharmacokinetics neurological disorders |
| title | Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders |
| title_full | Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders |
| title_fullStr | Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders |
| title_full_unstemmed | Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders |
| title_short | Mesenchymal stem cell-derived extracellular vesicles: current advances in preparation and therapeutic applications for neurological disorders |
| title_sort | mesenchymal stem cell derived extracellular vesicles current advances in preparation and therapeutic applications for neurological disorders |
| topic | mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) preparation characterization pharmacokinetics neurological disorders |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1626996/full |
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