Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on
Introduction and objectives: Klebsiella pneumoniae is an opportunistic pathogen that plays a major role in the development of nosocomial infections. One of the most important mechanisms of resistance to beta-lactam antibiotics is the production of beta-lactamase enzymes by this bacterium. The aim of...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Alborz University of Medical Sciences
2024-08-01
|
| Series: | نشریه دانشگاه علوم پزشکی البرز |
| Subjects: | |
| Online Access: | http://aums.abzums.ac.ir/article-1-1652-en.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846172774261850112 |
|---|---|
| author | Khorshid Jirdashtani Fatemeh Mousavi Leila Asadpour |
| author_facet | Khorshid Jirdashtani Fatemeh Mousavi Leila Asadpour |
| author_sort | Khorshid Jirdashtani |
| collection | DOAJ |
| description | Introduction and objectives: Klebsiella pneumoniae is an opportunistic pathogen that plays a major role in the development of nosocomial infections. One of the most important mechanisms of resistance to beta-lactam antibiotics is the production of beta-lactamase enzymes by this bacterium. The aim of this study was to determine the frequency of AmpC beta-lactamase producing clinical isolates of K. pneumoniae in Guilan and investigating the inhibitory effect thymol on these isolates.
Methods: Sixty isolates of K. pneumoniae were isolated and identified from clinical laboratories in Rasht during 2022. The pattern of antibiotic susceptibility in isolates was determined and beta-lactamase production was investigated using combined disk method. The presence of AmpC encoding genes including FOX, CITM and DHAM beta-lactamase genes in isolates was determined by PCR. The growth inhibitory effect of thymol on these isolates was determined using disck diffusion and broth microdilution and the efflux inhibitory effect was determined by Cartwheel methods.
Results: Among tested isolates 46 (76.6%) showed multi drug resistant phenotype. Of the 22 phenotypically AmpC-producing isolates, CITM and DHAM genes were detected in 12 (20%) and 7 (11.66%) isolates respectively and 4 isolates had both CITM and DHAM genes at the same time. Thymol, showed growth and efflux inhibitory effects against all tested K. pneumoniae isolates.
Conclusion: The results of the present study suggest the necessity of finding a successful treatment option to prevent the spread of infections caused by AmpC producing K. pneumoniae. According to the results, the use of thymol can provide a basis for the development of a new treatment against multi-drug resistant Klebsiella pneumoniae. |
| format | Article |
| id | doaj-art-e84159acf391481fa1c2528d3a5f0143 |
| institution | Kabale University |
| issn | 2322-3839 2588-3046 |
| language | fas |
| publishDate | 2024-08-01 |
| publisher | Alborz University of Medical Sciences |
| record_format | Article |
| series | نشریه دانشگاه علوم پزشکی البرز |
| spelling | doaj-art-e84159acf391481fa1c2528d3a5f01432024-11-09T06:27:57ZfasAlborz University of Medical Sciencesنشریه دانشگاه علوم پزشکی البرز2322-38392588-30462024-08-01133182193Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol onKhorshid Jirdashtani0Fatemeh Mousavi1Leila Asadpour2 Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran Introduction and objectives: Klebsiella pneumoniae is an opportunistic pathogen that plays a major role in the development of nosocomial infections. One of the most important mechanisms of resistance to beta-lactam antibiotics is the production of beta-lactamase enzymes by this bacterium. The aim of this study was to determine the frequency of AmpC beta-lactamase producing clinical isolates of K. pneumoniae in Guilan and investigating the inhibitory effect thymol on these isolates. Methods: Sixty isolates of K. pneumoniae were isolated and identified from clinical laboratories in Rasht during 2022. The pattern of antibiotic susceptibility in isolates was determined and beta-lactamase production was investigated using combined disk method. The presence of AmpC encoding genes including FOX, CITM and DHAM beta-lactamase genes in isolates was determined by PCR. The growth inhibitory effect of thymol on these isolates was determined using disck diffusion and broth microdilution and the efflux inhibitory effect was determined by Cartwheel methods. Results: Among tested isolates 46 (76.6%) showed multi drug resistant phenotype. Of the 22 phenotypically AmpC-producing isolates, CITM and DHAM genes were detected in 12 (20%) and 7 (11.66%) isolates respectively and 4 isolates had both CITM and DHAM genes at the same time. Thymol, showed growth and efflux inhibitory effects against all tested K. pneumoniae isolates. Conclusion: The results of the present study suggest the necessity of finding a successful treatment option to prevent the spread of infections caused by AmpC producing K. pneumoniae. According to the results, the use of thymol can provide a basis for the development of a new treatment against multi-drug resistant Klebsiella pneumoniae.http://aums.abzums.ac.ir/article-1-1652-en.pdfklebsiella pneumoniaeampc beta-lactamaseantibiotic resistancethymol |
| spellingShingle | Khorshid Jirdashtani Fatemeh Mousavi Leila Asadpour Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on نشریه دانشگاه علوم پزشکی البرز klebsiella pneumoniae ampc beta-lactamase antibiotic resistance thymol |
| title | Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on |
| title_full | Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on |
| title_fullStr | Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on |
| title_full_unstemmed | Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on |
| title_short | Identification of AmpC beta-lactamase producing clinical isolates of Klebsiella pneumoniae in Guilan and investigation of inhibitory effect of thymol on |
| title_sort | identification of ampc beta lactamase producing clinical isolates of klebsiella pneumoniae in guilan and investigation of inhibitory effect of thymol on |
| topic | klebsiella pneumoniae ampc beta-lactamase antibiotic resistance thymol |
| url | http://aums.abzums.ac.ir/article-1-1652-en.pdf |
| work_keys_str_mv | AT khorshidjirdashtani identificationofampcbetalactamaseproducingclinicalisolatesofklebsiellapneumoniaeinguilanandinvestigationofinhibitoryeffectofthymolon AT fatemehmousavi identificationofampcbetalactamaseproducingclinicalisolatesofklebsiellapneumoniaeinguilanandinvestigationofinhibitoryeffectofthymolon AT leilaasadpour identificationofampcbetalactamaseproducingclinicalisolatesofklebsiellapneumoniaeinguilanandinvestigationofinhibitoryeffectofthymolon |