Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats
<b>Aim:</b> The aim of this study was to determine the effect of macrolide antibiotics using in veterinary area on serum/ plasma inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats.<p> <b>Materials and Methods:</b> Total 1...
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=552 |
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| author | Ayşe Er Enver Yazar |
| author_facet | Ayşe Er Enver Yazar |
| author_sort | Ayşe Er |
| collection | DOAJ |
| description | <b>Aim:</b> The aim of this study was to determine the effect of
macrolide antibiotics using in veterinary area on serum/
plasma inflammatory mediators and organ damage markers
in lipopolysaccharide-induced pulmonary damage rats.<p>
<b>Materials and Methods:</b> Total 198 Sprague-Dawley rats
were used. Six rats were used for a 0th sampling point for
all groups. 192 rats were divided into four equal groups as
lipopolysaccharide (Escherichia coli 0111:B4, 0.5 mg/200
μl, intratracheal), lipopolysaccharide + tylosin (10 mg/kg,
subcutaneously), lipopolysaccharide + tilmicosin (20 mg/
kg, subcutaneously) and lipopolysaccharide + tulathromycin
(2.5 mg/kg, subcutaneously). After the treatments,
blood samples were collected by cardiac puncture under
anesthesia at 0.5, 1, 1.5, 2, 4, 6, 12 and 24 hours. Serum
tumor necrosis factor α, interleukin-1β, interleukin-6, interleukin-
10 levels, and plasma 13, 14-dihydro-15-ketoprostaglandin
F<sub>2α</sub> levels were determined by ELISA, while
serum C-reactive protein, creatine kinase-MB, aspartate
aminotransferase, alanine aminotransferase, alkaline phosphatase,
cholesterol, triglyceride, blood urea nitrogen and
creatinine levels were determined by otoanalyzer. Area
under the concentration time curve (AUC<sub>0-24</sub>), maximal
concentration in plasma or serum (C<sub>max</sub>) and time to reach
Cmax (t<sub>max</sub>) values were determined by pharmacokinetic
computer program.<p>
<b>Results:</b> Macrolide antibiotics increased serum tumor
necrosis factor α and plasma 13, 14-dihydro-15-keto-prostaglandin
F<sub>2α</sub> levels.<p>
<b>Conclusion:</b> Macrolide antibiotics used in veterinary area
may no shown depression on immune system. Hovewer, investigation
is need that especially dose-depended manner
effects of macrolide antibiotics on immunosystem. |
| format | Article |
| id | doaj-art-e80832a823934e0c9b81a7a3a44c07ff |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-e80832a823934e0c9b81a7a3a44c07ff2025-01-03T01:36:14ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-1953261713552Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage ratsAyşe ErEnver Yazar<b>Aim:</b> The aim of this study was to determine the effect of macrolide antibiotics using in veterinary area on serum/ plasma inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats.<p> <b>Materials and Methods:</b> Total 198 Sprague-Dawley rats were used. Six rats were used for a 0th sampling point for all groups. 192 rats were divided into four equal groups as lipopolysaccharide (Escherichia coli 0111:B4, 0.5 mg/200 μl, intratracheal), lipopolysaccharide + tylosin (10 mg/kg, subcutaneously), lipopolysaccharide + tilmicosin (20 mg/ kg, subcutaneously) and lipopolysaccharide + tulathromycin (2.5 mg/kg, subcutaneously). After the treatments, blood samples were collected by cardiac puncture under anesthesia at 0.5, 1, 1.5, 2, 4, 6, 12 and 24 hours. Serum tumor necrosis factor α, interleukin-1β, interleukin-6, interleukin- 10 levels, and plasma 13, 14-dihydro-15-ketoprostaglandin F<sub>2α</sub> levels were determined by ELISA, while serum C-reactive protein, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, cholesterol, triglyceride, blood urea nitrogen and creatinine levels were determined by otoanalyzer. Area under the concentration time curve (AUC<sub>0-24</sub>), maximal concentration in plasma or serum (C<sub>max</sub>) and time to reach Cmax (t<sub>max</sub>) values were determined by pharmacokinetic computer program.<p> <b>Results:</b> Macrolide antibiotics increased serum tumor necrosis factor α and plasma 13, 14-dihydro-15-keto-prostaglandin F<sub>2α</sub> levels.<p> <b>Conclusion:</b> Macrolide antibiotics used in veterinary area may no shown depression on immune system. Hovewer, investigation is need that especially dose-depended manner effects of macrolide antibiotics on immunosystem.http://eurasianjvetsci.org/pdf.php3?id=552lipopolysaccharidemacrolide antibioticinflammatory mediatorsorgan damage markers |
| spellingShingle | Ayşe Er Enver Yazar Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats Eurasian Journal of Veterinary Sciences lipopolysaccharide macrolide antibiotic inflammatory mediators organ damage markers |
| title | Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats |
| title_full | Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats |
| title_fullStr | Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats |
| title_full_unstemmed | Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats |
| title_short | Effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide-induced pulmonary damage rats |
| title_sort | effects of macrolide antibiotics on blood inflammatory mediators and organ damage markers in lipopolysaccharide induced pulmonary damage rats |
| topic | lipopolysaccharide macrolide antibiotic inflammatory mediators organ damage markers |
| url | http://eurasianjvetsci.org/pdf.php3?id=552 |
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