Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions
Trypsin inhibitors (TIs) in soybean seeds reduce the availability of processed soybean foods and animal feed. This study aimed to evaluate the trypsin inhibitor activity (TIA) in 999 Korean soybean accessions and conduct molecular characterization of soybean accessions with low TIA. TIA was evaluate...
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2023-10-01
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| author | Aron Park Se-Hee Kang Byeong-Hee Kang Sreeparna Chowdhury Seo-Young Shin Won-Ho Lee Jeong-Dong Lee Sungwoo Lee Yu-Mi Choi Bo-Keun Ha |
| author_facet | Aron Park Se-Hee Kang Byeong-Hee Kang Sreeparna Chowdhury Seo-Young Shin Won-Ho Lee Jeong-Dong Lee Sungwoo Lee Yu-Mi Choi Bo-Keun Ha |
| author_sort | Aron Park |
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| description | Trypsin inhibitors (TIs) in soybean seeds reduce the availability of processed soybean foods and animal feed. This study aimed to evaluate the trypsin inhibitor activity (TIA) in 999 Korean soybean accessions and conduct molecular characterization of soybean accessions with low TIA. TIA was evaluated using colorimetric analysis through a substrate–enzyme reaction. The average TIA of the 999 soybean accessions was 90.31%, ranging from 43.70% to 99.51%. Kunitz trypsin inhibitor 3 (KTi-3) gene target sequencing analysis was performed on seven soybean germplasm accessions (IT105782, IT170889, IT273590, IT274513, IT274515, IT276197, and IT022891) showing less than 60% TIA. Four soybean accessions (IT274513, IT274515, IT276197, and IT022891) exhibited the same mutations (a G-to-T transversion and an AG deletion) in the KTi-3 gene (<i>Glyma.08g341500</i>) in PI542044. In addition, whole-genome re-sequencing was performed on three soybean accessions with no mutations in the KTi-3 gene. Compared with the reference soybean genome, an in-frame insertion and five missense mutations were identified in the coding sequencing of the KTi-1 gene (<i>Glyma.01g09500</i>) in IT105782. A RT-qPCR analysis showed that the mRNA expression level of KTi-1 was reduced by approximately 17% in IT105782 during seed development. In this study, we identified a previously unreported mutation in the KTi-1 gene and developed a KASP marker using this new KTi-1 variant. |
| format | Article |
| id | doaj-art-e7f99234d6ba41f5bf7987b3ba3ac37b |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-e7f99234d6ba41f5bf7987b3ba3ac37b2024-12-16T13:35:15ZengMDPI AGAgriculture2077-04722023-10-011311207010.3390/agriculture13112070Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean AccessionsAron Park0Se-Hee Kang1Byeong-Hee Kang2Sreeparna Chowdhury3Seo-Young Shin4Won-Ho Lee5Jeong-Dong Lee6Sungwoo Lee7Yu-Mi Choi8Bo-Keun Ha9Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaTrypsin inhibitors (TIs) in soybean seeds reduce the availability of processed soybean foods and animal feed. This study aimed to evaluate the trypsin inhibitor activity (TIA) in 999 Korean soybean accessions and conduct molecular characterization of soybean accessions with low TIA. TIA was evaluated using colorimetric analysis through a substrate–enzyme reaction. The average TIA of the 999 soybean accessions was 90.31%, ranging from 43.70% to 99.51%. Kunitz trypsin inhibitor 3 (KTi-3) gene target sequencing analysis was performed on seven soybean germplasm accessions (IT105782, IT170889, IT273590, IT274513, IT274515, IT276197, and IT022891) showing less than 60% TIA. Four soybean accessions (IT274513, IT274515, IT276197, and IT022891) exhibited the same mutations (a G-to-T transversion and an AG deletion) in the KTi-3 gene (<i>Glyma.08g341500</i>) in PI542044. In addition, whole-genome re-sequencing was performed on three soybean accessions with no mutations in the KTi-3 gene. Compared with the reference soybean genome, an in-frame insertion and five missense mutations were identified in the coding sequencing of the KTi-1 gene (<i>Glyma.01g09500</i>) in IT105782. A RT-qPCR analysis showed that the mRNA expression level of KTi-1 was reduced by approximately 17% in IT105782 during seed development. In this study, we identified a previously unreported mutation in the KTi-1 gene and developed a KASP marker using this new KTi-1 variant.https://www.mdpi.com/2077-0472/13/11/2070soybeantrypsin inhibitor (TI)trypsin inhibitor activity (TIA)KASP marker |
| spellingShingle | Aron Park Se-Hee Kang Byeong-Hee Kang Sreeparna Chowdhury Seo-Young Shin Won-Ho Lee Jeong-Dong Lee Sungwoo Lee Yu-Mi Choi Bo-Keun Ha Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions Agriculture soybean trypsin inhibitor (TI) trypsin inhibitor activity (TIA) KASP marker |
| title | Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions |
| title_full | Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions |
| title_fullStr | Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions |
| title_full_unstemmed | Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions |
| title_short | Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions |
| title_sort | identification of a novel kti 1 allele associated with reduced trypsin inhibitor activity in soybean accessions |
| topic | soybean trypsin inhibitor (TI) trypsin inhibitor activity (TIA) KASP marker |
| url | https://www.mdpi.com/2077-0472/13/11/2070 |
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