Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor
<i>Escherichia coli (E. coli)</i> detection suffers from slow analysis time and high costs, along with the need for specificity. While state-of-the-art electrochemical biosensors are cost-efficient and easy to implement, their sensitivity and analysis time still require improvement. In t...
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MDPI AG
2024-12-01
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| Series: | Biosensors |
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| author | Furkan Soysaldı Derya Dincyurek Ekici Mehmet Çağrı Soylu Evren Mutlugun |
| author_facet | Furkan Soysaldı Derya Dincyurek Ekici Mehmet Çağrı Soylu Evren Mutlugun |
| author_sort | Furkan Soysaldı |
| collection | DOAJ |
| description | <i>Escherichia coli (E. coli)</i> detection suffers from slow analysis time and high costs, along with the need for specificity. While state-of-the-art electrochemical biosensors are cost-efficient and easy to implement, their sensitivity and analysis time still require improvement. In this work, we present a paper-based electrochemical biosensor utilizing magnetic core-shell Fe<sub>2</sub>O<sub>3</sub>@CdSe/ZnS quantum dots (MQDs) to achieve fast detection, low cost, and high sensitivity. Using electrochemical impedance spectroscopy (EIS) as the detection technique, the biosensor achieved a limit of detection of 2.7 × 10<sup>2</sup> CFU/mL for <i>E. coli</i> bacteria across a concentration range of 10<sup>2</sup>–10<sup>8</sup> CFU/mL, with a relative standard deviation (RSD) of 3.5781%. From an optical perspective, as <i>E. coli</i> concentration increased steadily from 10<sup>4</sup> to 10<sup>7</sup> CFU/mL, quantum dot fluorescence showed over 60% lifetime quenching. This hybrid biosensor thus provides rapid, highly sensitive <i>E. coli</i> detection with a fast analysis time of 30 min. This study, which combines the detection advantages of electrochemical and optical biosensor systems in a graphite-based paper sensor for the first time, has the potential to meet the needs of point-of-care applications. It is thought that future studies that will aim to examine the performance of the production-optimized, portable, graphite-based sensor system on real food samples, environmental samples, and especially medical clinical samples will be promising. |
| format | Article |
| id | doaj-art-e7e2526bedd74272b18b2ef9efa0ca6d |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-e7e2526bedd74272b18b2ef9efa0ca6d2024-12-27T14:14:15ZengMDPI AGBiosensors2079-63742024-12-01141260310.3390/bios14120603Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper BiosensorFurkan Soysaldı0Derya Dincyurek Ekici1Mehmet Çağrı Soylu2Evren Mutlugun3Biological and Medical Diagnostic Sensors Laboratory (BioMeD Sensors Lab), Department of Biomedical Engineering, Erciyes University, Kayseri 38030, TürkiyeDepartment of Nanotechnology Engineering, Abdullah Gul University, Kayseri 38039, TürkiyeBiological and Medical Diagnostic Sensors Laboratory (BioMeD Sensors Lab), Department of Biomedical Engineering, Erciyes University, Kayseri 38030, TürkiyeDepartment of Electrical-Electronics Engineering, Abdullah Gul University, Kayseri 38039, Türkiye<i>Escherichia coli (E. coli)</i> detection suffers from slow analysis time and high costs, along with the need for specificity. While state-of-the-art electrochemical biosensors are cost-efficient and easy to implement, their sensitivity and analysis time still require improvement. In this work, we present a paper-based electrochemical biosensor utilizing magnetic core-shell Fe<sub>2</sub>O<sub>3</sub>@CdSe/ZnS quantum dots (MQDs) to achieve fast detection, low cost, and high sensitivity. Using electrochemical impedance spectroscopy (EIS) as the detection technique, the biosensor achieved a limit of detection of 2.7 × 10<sup>2</sup> CFU/mL for <i>E. coli</i> bacteria across a concentration range of 10<sup>2</sup>–10<sup>8</sup> CFU/mL, with a relative standard deviation (RSD) of 3.5781%. From an optical perspective, as <i>E. coli</i> concentration increased steadily from 10<sup>4</sup> to 10<sup>7</sup> CFU/mL, quantum dot fluorescence showed over 60% lifetime quenching. This hybrid biosensor thus provides rapid, highly sensitive <i>E. coli</i> detection with a fast analysis time of 30 min. This study, which combines the detection advantages of electrochemical and optical biosensor systems in a graphite-based paper sensor for the first time, has the potential to meet the needs of point-of-care applications. It is thought that future studies that will aim to examine the performance of the production-optimized, portable, graphite-based sensor system on real food samples, environmental samples, and especially medical clinical samples will be promising.https://www.mdpi.com/2079-6374/14/12/603magneto-opticbiosensorelectrochemical impedance spectroscopyquantum dotsFe<sub>2</sub>O<sub>3</sub>@CdSe/ZnS |
| spellingShingle | Furkan Soysaldı Derya Dincyurek Ekici Mehmet Çağrı Soylu Evren Mutlugun Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor Biosensors magneto-optic biosensor electrochemical impedance spectroscopy quantum dots Fe<sub>2</sub>O<sub>3</sub>@CdSe/ZnS |
| title | Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor |
| title_full | Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor |
| title_fullStr | Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor |
| title_full_unstemmed | Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor |
| title_short | Electrochemical and Optical Multi-Detection of <i>Escherichia coli</i> Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor |
| title_sort | electrochemical and optical multi detection of i escherichia coli i through magneto optic nanoparticles a pencil on paper biosensor |
| topic | magneto-optic biosensor electrochemical impedance spectroscopy quantum dots Fe<sub>2</sub>O<sub>3</sub>@CdSe/ZnS |
| url | https://www.mdpi.com/2079-6374/14/12/603 |
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