Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i>
Symbiotic microbiota significantly influence the development, physiology, and behavior of their hosts, and therefore, they are widely studied. However, very few studies have investigated the changes in symbiotic microbiota across generations. <i>Daphnia magna</i> originating from the Qin...
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2024-12-01
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| author | Minru You Wenwu Yang |
| author_facet | Minru You Wenwu Yang |
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| description | Symbiotic microbiota significantly influence the development, physiology, and behavior of their hosts, and therefore, they are widely studied. However, very few studies have investigated the changes in symbiotic microbiota across generations. <i>Daphnia magna</i> originating from the Qinghai–Tibetan Plateau were cultured through seven generations in our laboratory, and the symbiotic microbiota of <i>D. magna</i> were sequenced using a 16S rRNA amplicon to analyze changes in the structure and functional properties of the symbiotic microbiota of <i>D. magna</i> from a harsh environment to an ideal environment. We detected substantial changes in the symbiotic microbiota of <i>D. magna</i> across generations. For example, the genus <i>Nevskia</i>, a member of the gamma-subclass Proteobacteria, had the highest abundance in the first generation (G1), followed by a decrease in abundance in the fourth (G4) and seventh (G7) generations. The gene functions of the microbiota in different generations of <i>D. magna</i> also changed significantly. The fourth generation was mainly rich in fatty acyl-CoA synthase, acetyl-CoA acyltransferase, phosphoglycerol phosphatase, etc. The seventh generation was mainly rich in osmotic enzyme protein and ATP-binding protein of the ABC transport system. This study confirms that the alterations in the structure and functional properties of the symbiotic microbiota of <i>D. magna</i> under changing environments are typical responses of <i>D. magna</i> to environmental changes. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-e7c97fb93d1f4f5aa799187eca79c46b2024-12-27T14:41:21ZengMDPI AGMicroorganisms2076-26072024-12-011212249210.3390/microorganisms12122492Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i>Minru You0Wenwu Yang1MOE Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Science, Fudan University, Songhu Road 2005, Shanghai 200438, ChinaMOE Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Science, Fudan University, Songhu Road 2005, Shanghai 200438, ChinaSymbiotic microbiota significantly influence the development, physiology, and behavior of their hosts, and therefore, they are widely studied. However, very few studies have investigated the changes in symbiotic microbiota across generations. <i>Daphnia magna</i> originating from the Qinghai–Tibetan Plateau were cultured through seven generations in our laboratory, and the symbiotic microbiota of <i>D. magna</i> were sequenced using a 16S rRNA amplicon to analyze changes in the structure and functional properties of the symbiotic microbiota of <i>D. magna</i> from a harsh environment to an ideal environment. We detected substantial changes in the symbiotic microbiota of <i>D. magna</i> across generations. For example, the genus <i>Nevskia</i>, a member of the gamma-subclass Proteobacteria, had the highest abundance in the first generation (G1), followed by a decrease in abundance in the fourth (G4) and seventh (G7) generations. The gene functions of the microbiota in different generations of <i>D. magna</i> also changed significantly. The fourth generation was mainly rich in fatty acyl-CoA synthase, acetyl-CoA acyltransferase, phosphoglycerol phosphatase, etc. The seventh generation was mainly rich in osmotic enzyme protein and ATP-binding protein of the ABC transport system. This study confirms that the alterations in the structure and functional properties of the symbiotic microbiota of <i>D. magna</i> under changing environments are typical responses of <i>D. magna</i> to environmental changes.https://www.mdpi.com/2076-2607/12/12/2492<i>Daphnia</i>symbiotic microbiota16S rRNAdiversityvariationstransgeneration |
| spellingShingle | Minru You Wenwu Yang Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i> Microorganisms <i>Daphnia</i> symbiotic microbiota 16S rRNA diversity variations transgeneration |
| title | Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i> |
| title_full | Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i> |
| title_fullStr | Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i> |
| title_full_unstemmed | Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i> |
| title_short | Environmental Changes Driving Shifts in the Structure and Functional Properties of the Symbiotic Microbiota of <i>Daphnia</i> |
| title_sort | environmental changes driving shifts in the structure and functional properties of the symbiotic microbiota of i daphnia i |
| topic | <i>Daphnia</i> symbiotic microbiota 16S rRNA diversity variations transgeneration |
| url | https://www.mdpi.com/2076-2607/12/12/2492 |
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