Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma
A simple and accurate method for the determination of ibuprofen concentrations in elephant plasma was developed and validated using reverse phase high-performance liquid chromatography (HPLC). Utilizing a liquid-liquid extraction with ethyl acetate, methanol, and phosphoric acid, samples were separa...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024151766 |
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| author | Sherry Cox Aaron Bloom Riley Golias Madeline Duncan Joan Bergman Andrew Cushing |
| author_facet | Sherry Cox Aaron Bloom Riley Golias Madeline Duncan Joan Bergman Andrew Cushing |
| author_sort | Sherry Cox |
| collection | DOAJ |
| description | A simple and accurate method for the determination of ibuprofen concentrations in elephant plasma was developed and validated using reverse phase high-performance liquid chromatography (HPLC). Utilizing a liquid-liquid extraction with ethyl acetate, methanol, and phosphoric acid, samples were separated on an XBridge C18 column. The mobile phase consisted of 0.005 M ammonium phosphate and acetonitrile (40:60, v/v). Both ibuprofen and the internal standard flurbiprofen were quantitated using an ultraviolet (UV) detector set at 214 nm. The lower limit of quantification (LLOQ) was 0.05 μg/mL in 100 μL of plasma. The average recovery was over 95 % while the intra-assay variability ranged from 0.8 to 9.9 % and the inter-assay variability ranged from 2.7 to 9.8 %. The method was used successfully in a therapeutic drug monitoring program for elephants and could be used in future pharmacokinetic studies. |
| format | Article |
| id | doaj-art-e79f57f3d5ca4ddfa2e3b08c2d6f8dde |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-e79f57f3d5ca4ddfa2e3b08c2d6f8dde2024-11-12T05:19:54ZengElsevierHeliyon2405-84402024-10-011020e39145Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasmaSherry Cox0Aaron Bloom1Riley Golias2Madeline Duncan3Joan Bergman4Andrew Cushing5The University of Tennessee, College of Veterinary Medicine, Department of Biomedical and Diagnostic Sciences, 2407 River Drive, Knoxville, TN 37996, USA; Corresponding author.The University of Tennessee, College of Veterinary Medicine, Department of Biomedical and Diagnostic Sciences, 2407 River Drive, Knoxville, TN 37996, USAThe University of Tennessee, College of Veterinary Medicine, Department of Biomedical and Diagnostic Sciences, 2407 River Drive, Knoxville, TN 37996, USAThe University of Tennessee, College of Veterinary Medicine, Department of Biomedical and Diagnostic Sciences, 2407 River Drive, Knoxville, TN 37996, USAThe University of Tennessee, College of Veterinary Medicine, Department of Biomedical and Diagnostic Sciences, 2407 River Drive, Knoxville, TN 37996, USAThe University of Tennessee, College of Veterinary Medicine, Department of Small Animal Clinical Sciences, 2407 River Drive, Knoxville, TN 37996, USAA simple and accurate method for the determination of ibuprofen concentrations in elephant plasma was developed and validated using reverse phase high-performance liquid chromatography (HPLC). Utilizing a liquid-liquid extraction with ethyl acetate, methanol, and phosphoric acid, samples were separated on an XBridge C18 column. The mobile phase consisted of 0.005 M ammonium phosphate and acetonitrile (40:60, v/v). Both ibuprofen and the internal standard flurbiprofen were quantitated using an ultraviolet (UV) detector set at 214 nm. The lower limit of quantification (LLOQ) was 0.05 μg/mL in 100 μL of plasma. The average recovery was over 95 % while the intra-assay variability ranged from 0.8 to 9.9 % and the inter-assay variability ranged from 2.7 to 9.8 %. The method was used successfully in a therapeutic drug monitoring program for elephants and could be used in future pharmacokinetic studies.http://www.sciencedirect.com/science/article/pii/S2405844024151766IbuprofenPlasmaUltraviolet detection |
| spellingShingle | Sherry Cox Aaron Bloom Riley Golias Madeline Duncan Joan Bergman Andrew Cushing Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma Heliyon Ibuprofen Plasma Ultraviolet detection |
| title | Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma |
| title_full | Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma |
| title_fullStr | Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma |
| title_full_unstemmed | Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma |
| title_short | Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma |
| title_sort | development validation and application of a high performance liquid chromatography method for the detection of ibuprofen in elephant plasma |
| topic | Ibuprofen Plasma Ultraviolet detection |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024151766 |
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