Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management
Investigating bacterial diversity in the soil of oil palm plantations is crucial for developing effective soil management strategies to mitigate greenhouse gas emissions, thereby addressing climate change challenges. In this study, we analysed soil bacterial communities within an oil palm plantation...
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EDP Sciences
2024-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/129/e3sconf_staclim2024_03004.pdf |
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| author | Zahidah Ayob Nor Azizah Kusai Han Ming Gan Wan Nurul‘Ain Wan Mohd Nor Hasimah Mos Vijaya Subramaniam Meilina Ong Abdullah |
| author_facet | Zahidah Ayob Nor Azizah Kusai Han Ming Gan Wan Nurul‘Ain Wan Mohd Nor Hasimah Mos Vijaya Subramaniam Meilina Ong Abdullah |
| author_sort | Zahidah Ayob |
| collection | DOAJ |
| description | Investigating bacterial diversity in the soil of oil palm plantations is crucial for developing effective soil management strategies to mitigate greenhouse gas emissions, thereby addressing climate change challenges. In this study, we analysed soil bacterial communities within an oil palm plantation in Sarawak, Malaysia. To capture the full spectrum of microbial diversity, samples were collected across three distinct soil depths and various managements zones within the plantation. We employed Illumina amplicon sequencing targeting the V3 region of the 16S rRNA gene, which revealed a diverse array of soil bacteria, primarily from the phyla Acidobacteriota, Actinobacteriota, and Proteobacteria. Notably, families such as RAAP2_2 and Isosphaeraceae were identified as key biomarkers predominantly inhabiting deeper soil layers (30-45 cm), while UBA7541, Streptosporangiaceae, and Sporichthyaceae were more prevalent in the surface layer (0-15 cm). Microbial correlation network analysis revealed a complex network of robust and significant interactions among 11 bacterial families, suggesting their involvement in plant material degradation and carbon cycling. Redundancy Analysis (RDA) revealed that pH, moisture content, and bulk density were significant factors that influence the microbial community structure. At the phylum level, Acidobacteriota showed a close association with pH, while Proteobacteria was associated with higher moisture content. Interestingly, we discovered that the soil was not as homogeneous as initially thought. Microenvironmental variability was evident, with specific groupings based on subplot sampled. These findings provide valuable insights into the factors shaping microbial communities in oil palm plantation soils, thus opening avenues for further exploration of the complex mechanisms driving ecosystem processes in this unique Malaysian soil environment. |
| format | Article |
| id | doaj-art-e78e8d2b7ec147939d4913827caae070 |
| institution | Kabale University |
| issn | 2267-1242 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj-art-e78e8d2b7ec147939d4913827caae0702025-01-16T11:22:27ZengEDP SciencesE3S Web of Conferences2267-12422024-01-015990300410.1051/e3sconf/202459903004e3sconf_staclim2024_03004Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil ManagementZahidah Ayob0Nor Azizah Kusai1Han Ming Gan2Wan Nurul‘Ain Wan Mohd Nor3Hasimah Mos4Vijaya Subramaniam5Meilina Ong Abdullah6Malaysian Palm Oil Board, No. 6, Persiaran Institut, Bandar Baru BangiMalaysian Palm Oil Board, No. 6, Persiaran Institut, Bandar Baru BangiPatriot Biotech Sdn. Bhd.Malaysian Palm Oil Board, No. 6, Persiaran Institut, Bandar Baru BangiMalaysian Palm Oil Board, No. 6, Persiaran Institut, Bandar Baru BangiMalaysian Palm Oil Board, No. 6, Persiaran Institut, Bandar Baru BangiMalaysian Palm Oil Board, No. 6, Persiaran Institut, Bandar Baru BangiInvestigating bacterial diversity in the soil of oil palm plantations is crucial for developing effective soil management strategies to mitigate greenhouse gas emissions, thereby addressing climate change challenges. In this study, we analysed soil bacterial communities within an oil palm plantation in Sarawak, Malaysia. To capture the full spectrum of microbial diversity, samples were collected across three distinct soil depths and various managements zones within the plantation. We employed Illumina amplicon sequencing targeting the V3 region of the 16S rRNA gene, which revealed a diverse array of soil bacteria, primarily from the phyla Acidobacteriota, Actinobacteriota, and Proteobacteria. Notably, families such as RAAP2_2 and Isosphaeraceae were identified as key biomarkers predominantly inhabiting deeper soil layers (30-45 cm), while UBA7541, Streptosporangiaceae, and Sporichthyaceae were more prevalent in the surface layer (0-15 cm). Microbial correlation network analysis revealed a complex network of robust and significant interactions among 11 bacterial families, suggesting their involvement in plant material degradation and carbon cycling. Redundancy Analysis (RDA) revealed that pH, moisture content, and bulk density were significant factors that influence the microbial community structure. At the phylum level, Acidobacteriota showed a close association with pH, while Proteobacteria was associated with higher moisture content. Interestingly, we discovered that the soil was not as homogeneous as initially thought. Microenvironmental variability was evident, with specific groupings based on subplot sampled. These findings provide valuable insights into the factors shaping microbial communities in oil palm plantation soils, thus opening avenues for further exploration of the complex mechanisms driving ecosystem processes in this unique Malaysian soil environment.https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/129/e3sconf_staclim2024_03004.pdf |
| spellingShingle | Zahidah Ayob Nor Azizah Kusai Han Ming Gan Wan Nurul‘Ain Wan Mohd Nor Hasimah Mos Vijaya Subramaniam Meilina Ong Abdullah Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management E3S Web of Conferences |
| title | Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management |
| title_full | Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management |
| title_fullStr | Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management |
| title_full_unstemmed | Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management |
| title_short | Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management |
| title_sort | bacteria assemble investigating soil bacterial diversity and interactions in a sarawakian oil palm plantation for climate resilient soil management |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/129/e3sconf_staclim2024_03004.pdf |
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