NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro
Background Interleukin (IL)-2 is a key cytokine capable of modulating the immune response by activating natural killer (NK) cells. This study was recruited to explore the therapeutic potential of IL-2-activated NK-92 cells in endometriosis in vitro.Methods Ectopic endometrial stromal cells (EESCs) w...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2372682 |
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| _version_ | 1841553188481662976 |
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| author | Jun Yao Li-jiao Zhang Zhe Zhou Mao-fang Hua |
| author_facet | Jun Yao Li-jiao Zhang Zhe Zhou Mao-fang Hua |
| author_sort | Jun Yao |
| collection | DOAJ |
| description | Background Interleukin (IL)-2 is a key cytokine capable of modulating the immune response by activating natural killer (NK) cells. This study was recruited to explore the therapeutic potential of IL-2-activated NK-92 cells in endometriosis in vitro.Methods Ectopic endometrial stromal cells (EESCs) were isolated and co-cultured with IL-2-activated NK-92 cells at varying effector-to-target (E:T) ratios (1:0 [Control], 1:1, 1:3, and 1:9). The viability, cytotoxicity, and cell surface antigen expression of IL-2-activated NK-92 cells were assessed. The viability, apoptosis, invasion, and migration ability of EESCs co-cultured with NK-92 cells at different ratios were evaluated. The apoptosis-related proteins, invasion and migration-related proteins as well as MEK/ERK pathway were examined via western blot. Each experiment was repeated three times.Results IL-2 activation enhanced NK-92 cytotoxicity in a concentration-dependent manner. Co-culturing EESCs with IL-2-activated NK-92 cells at E:T ratios of 1:1, 1:3, and 1:9 reduced EESC viability by 20%, 45%, and 70%, respectively, compared to the control group. Apoptosis rates in EESCs increased in correlation with the NK-92 cell proportion, with the highest rate observed at a 1:9 ratio. Moreover, EESC invasion and migration were significantly inhibited by IL-2-activated NK-92 cells, with a 60% reduction in invasion and a 50% decrease in migration at the 1:9 ratio. Besides, the MEK/ERK signalling pathway was down-regulated in EESCs by IL-2-activated NK-92 cells.Conclusion IL-2-activated NK-92 cells exhibit potent cytotoxic effects against EESCs. They promote EESC apoptosis and inhibit viability, invasion, and migration through modulating the MEK/ERK signalling pathway. |
| format | Article |
| id | doaj-art-e76f7f859e7e4f288096a94787673ab1 |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-e76f7f859e7e4f288096a94787673ab12025-01-09T12:13:17ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2372682NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitroJun Yao0Li-jiao Zhang1Zhe Zhou2Mao-fang Hua3Department of Gynecology, Lianyungang Maternal and Child Health Hospital, Lianyungang, Jiangsu, ChinaDepartment of Obstetrics, Lianyungang Maternal and Child Health Hospital, Lianyungang, Jiangsu, ChinaDepartment of Gynecology, Lianyungang Maternal and Child Health Hospital, Lianyungang, Jiangsu, ChinaDepartment of Gynecology, Lianyungang Maternal and Child Health Hospital, Lianyungang, Jiangsu, ChinaBackground Interleukin (IL)-2 is a key cytokine capable of modulating the immune response by activating natural killer (NK) cells. This study was recruited to explore the therapeutic potential of IL-2-activated NK-92 cells in endometriosis in vitro.Methods Ectopic endometrial stromal cells (EESCs) were isolated and co-cultured with IL-2-activated NK-92 cells at varying effector-to-target (E:T) ratios (1:0 [Control], 1:1, 1:3, and 1:9). The viability, cytotoxicity, and cell surface antigen expression of IL-2-activated NK-92 cells were assessed. The viability, apoptosis, invasion, and migration ability of EESCs co-cultured with NK-92 cells at different ratios were evaluated. The apoptosis-related proteins, invasion and migration-related proteins as well as MEK/ERK pathway were examined via western blot. Each experiment was repeated three times.Results IL-2 activation enhanced NK-92 cytotoxicity in a concentration-dependent manner. Co-culturing EESCs with IL-2-activated NK-92 cells at E:T ratios of 1:1, 1:3, and 1:9 reduced EESC viability by 20%, 45%, and 70%, respectively, compared to the control group. Apoptosis rates in EESCs increased in correlation with the NK-92 cell proportion, with the highest rate observed at a 1:9 ratio. Moreover, EESC invasion and migration were significantly inhibited by IL-2-activated NK-92 cells, with a 60% reduction in invasion and a 50% decrease in migration at the 1:9 ratio. Besides, the MEK/ERK signalling pathway was down-regulated in EESCs by IL-2-activated NK-92 cells.Conclusion IL-2-activated NK-92 cells exhibit potent cytotoxic effects against EESCs. They promote EESC apoptosis and inhibit viability, invasion, and migration through modulating the MEK/ERK signalling pathway.https://www.tandfonline.com/doi/10.1080/01443615.2024.2372682Endometriosisectopic endometrial stromal cellsNK-92 cellsIL-2MEK/ERK signalling pathway |
| spellingShingle | Jun Yao Li-jiao Zhang Zhe Zhou Mao-fang Hua NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro Journal of Obstetrics and Gynaecology Endometriosis ectopic endometrial stromal cells NK-92 cells IL-2 MEK/ERK signalling pathway |
| title | NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro |
| title_full | NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro |
| title_fullStr | NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro |
| title_full_unstemmed | NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro |
| title_short | NK-92 cells activated by IL-2 inhibit the progression of endometriosis in vitro |
| title_sort | nk 92 cells activated by il 2 inhibit the progression of endometriosis in vitro |
| topic | Endometriosis ectopic endometrial stromal cells NK-92 cells IL-2 MEK/ERK signalling pathway |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2372682 |
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