Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices

Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices

Abstract Foodborne bacteria like Escherichia coli threaten global food security, necessitating affordable, on-site detection methods, especially in resource-limited settings. This study optimized loop-mediated isothermal amplification (LAMP) integrated with peroxidase-mimicking G-quadruplex DNA stru...

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Bibliographic Details
Main Authors: Alaa H. Sewid, Haley C. Dylewski, Joseph H. Ramos, Bailey M. Morgan, Benti D. Gelalcha, Doris H. D’Souza, Jie Jayne Wu, Oudessa Kerro Dego, Shigetoshi Eda
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Food safety
Loop mediated isothermal amplification (LAMP)
E. coli
G-quadruplex DNAzyme
Magnetic beads
Electrochemical sensing
Online Access:https://doi.org/10.1038/s41598-024-80392-4
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