Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019
The rise in the prevalence of carbapenem resistant bacteria worldwide has made the treatment of nosocomial infections challenging. This is because carbapenem resistant bacteria harbor multiple resistance genes aside the carbapenemase genes. This study was conducted to determine the susceptibility pa...
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Elsevier
2024-12-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194624001419 |
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| author | Ibrahim Mohammed Hussaini Ahmed Babagida Suleiman Olayeni Stephen Olonitola Rukayat Avosuahi Oyi |
| author_facet | Ibrahim Mohammed Hussaini Ahmed Babagida Suleiman Olayeni Stephen Olonitola Rukayat Avosuahi Oyi |
| author_sort | Ibrahim Mohammed Hussaini |
| collection | DOAJ |
| description | The rise in the prevalence of carbapenem resistant bacteria worldwide has made the treatment of nosocomial infections challenging. This is because carbapenem resistant bacteria harbor multiple resistance genes aside the carbapenemase genes. This study was conducted to determine the susceptibility pattern of carbapenem resistant isolates of Escherichia coli and Klebsiella pneumoniae. Previously isolated and characterized carbapenem resistant E. coli and K. pneumoniae were screened for their susceptibility to ceftriaxone, trimethoprim-sulphamethoxazole, doxycycline, amikacin, gentamicin, nalidixic acid, chloramphenicol, colistin, tigecycline and fosfomycin by the Kirby Bauer technique. EUCAST and CLSI breakpoints were used to interpret the zones of inhibition. High level of resistance was observed among the isolates to ceftriaxone (100.00 %), trimethoprim-sulphamethoxazole (100.00 %) and doxycycline (83.33 %). Two-third of the isolates (66.67 %) were observed to be susceptible to amikacin, while 33.33 % of the isolates were gentamicin and nalidixic acid susceptible. Susceptibility to chloramphenicol and colistin was recorded in 16.67 % of the isolates while all the screened isolates (100.0 %) were susceptible to fosfomycin and tigecycline. A higher antibiotic resistance rate was observed among isolates habouring carbapenemase genes (blaNDM, blaOXA or both). The MAR indices of the carbapenem resistant isolates ranged between 0.46 and 0.82. So also, all the isolates screened were observed to be resistant to multiple antibiotics, hence MDR isolates. In conclusion, the carbapenem resistant isolates were resistant to multiple antibiotics but were however susceptible to tigecycline, fosfomycin and amikacin. |
| format | Article |
| id | doaj-art-e6feac0b1cfd4fce96d0690d679faad4 |
| institution | Kabale University |
| issn | 2950-1946 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-e6feac0b1cfd4fce96d0690d679faad42024-12-18T08:55:41ZengElsevierThe Microbe2950-19462024-12-015100174Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019Ibrahim Mohammed Hussaini0Ahmed Babagida Suleiman1Olayeni Stephen Olonitola2Rukayat Avosuahi Oyi3Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria; Corresponding author.Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, NigeriaDepartment of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, NigeriaDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, NigeriaThe rise in the prevalence of carbapenem resistant bacteria worldwide has made the treatment of nosocomial infections challenging. This is because carbapenem resistant bacteria harbor multiple resistance genes aside the carbapenemase genes. This study was conducted to determine the susceptibility pattern of carbapenem resistant isolates of Escherichia coli and Klebsiella pneumoniae. Previously isolated and characterized carbapenem resistant E. coli and K. pneumoniae were screened for their susceptibility to ceftriaxone, trimethoprim-sulphamethoxazole, doxycycline, amikacin, gentamicin, nalidixic acid, chloramphenicol, colistin, tigecycline and fosfomycin by the Kirby Bauer technique. EUCAST and CLSI breakpoints were used to interpret the zones of inhibition. High level of resistance was observed among the isolates to ceftriaxone (100.00 %), trimethoprim-sulphamethoxazole (100.00 %) and doxycycline (83.33 %). Two-third of the isolates (66.67 %) were observed to be susceptible to amikacin, while 33.33 % of the isolates were gentamicin and nalidixic acid susceptible. Susceptibility to chloramphenicol and colistin was recorded in 16.67 % of the isolates while all the screened isolates (100.0 %) were susceptible to fosfomycin and tigecycline. A higher antibiotic resistance rate was observed among isolates habouring carbapenemase genes (blaNDM, blaOXA or both). The MAR indices of the carbapenem resistant isolates ranged between 0.46 and 0.82. So also, all the isolates screened were observed to be resistant to multiple antibiotics, hence MDR isolates. In conclusion, the carbapenem resistant isolates were resistant to multiple antibiotics but were however susceptible to tigecycline, fosfomycin and amikacin.http://www.sciencedirect.com/science/article/pii/S2950194624001419Carbapenem resistanceAntibioticSusceptibilityE. coliK. pneumoniae |
| spellingShingle | Ibrahim Mohammed Hussaini Ahmed Babagida Suleiman Olayeni Stephen Olonitola Rukayat Avosuahi Oyi Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019 The Microbe Carbapenem resistance Antibiotic Susceptibility E. coli K. pneumoniae |
| title | Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019 |
| title_full | Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019 |
| title_fullStr | Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019 |
| title_full_unstemmed | Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019 |
| title_short | Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019 |
| title_sort | antibiotic susceptibility pattern of carbapenem resistant escherichia coli and klebsiella pneumoniae 2019 |
| topic | Carbapenem resistance Antibiotic Susceptibility E. coli K. pneumoniae |
| url | http://www.sciencedirect.com/science/article/pii/S2950194624001419 |
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