Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat
Objective In this study, we established a Sprague-Dawley rat model of vulvar squamous intraepithelial lesions and investigated the impact of focused ultrasound on the expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and mutant type p53 (mtp53) in the vulv...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | International Journal of Hyperthermia |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2024.2401417 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841560928511852544 |
|---|---|
| author | Yijin Fan Xi Wang Yao Liu Huajun Tang Chengzhi Li |
| author_facet | Yijin Fan Xi Wang Yao Liu Huajun Tang Chengzhi Li |
| author_sort | Yijin Fan |
| collection | DOAJ |
| description | Objective In this study, we established a Sprague-Dawley rat model of vulvar squamous intraepithelial lesions and investigated the impact of focused ultrasound on the expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and mutant type p53 (mtp53) in the vulvar skin of rats with low-grade squamous intraepithelial lesions (LSIL).Materials and methods The vulvar skin of 60 rats was treated with dimethylbenzanthracene (DMBA) and mechanical irritation three times a week for 14 weeks. Rats with LSIL were randomly allocated into the experimental group or the control group. The experimental group was treated with focused ultrasound, while the control group received sham treatment.Results After 14 weeks treatment of DMBA combined with mechanical irritation, LSIL were observed in 44 (73.33%) rats, and high-grade squamous intraepithelial lesions (HSIL) were observed in 14 (23.33%) rats. 90.91% (20/22) of rats showed normal pathology and 9.09% (2/22) of rats exhibited LSIL in the experimental group at four weeks after focused ultrasound treatment. 22.73% (5/22) of rats exhibited LSIL, 77.27% (17/22) of rats progressed to HSIL in the control group. Compared with the control-group rats, the levels of HIF-1α, VEGF and mtp53 were significantly decreased in experimental-group rats (p < 0.05).Conclusions These results indicate that DMBA combined with mechanical irritation can induce vulvar squamous intraepithelial lesion in SD rats. Focused ultrasound can treat LSIL safely and effectively, prevent the progression of vulvar lesions, and improve the microenvironment of vulvar tissues by decreasing the localized expression of HIF-1α, VEGF, and mtp53 in rats. |
| format | Article |
| id | doaj-art-e6e9f4097e304a7bb99d76bbc30f0479 |
| institution | Kabale University |
| issn | 0265-6736 1464-5157 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj-art-e6e9f4097e304a7bb99d76bbc30f04792025-01-03T09:30:27ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572024-12-0141110.1080/02656736.2024.2401417Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in ratYijin Fan0Xi Wang1Yao Liu2Huajun Tang3Chengzhi Li4State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaObjective In this study, we established a Sprague-Dawley rat model of vulvar squamous intraepithelial lesions and investigated the impact of focused ultrasound on the expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and mutant type p53 (mtp53) in the vulvar skin of rats with low-grade squamous intraepithelial lesions (LSIL).Materials and methods The vulvar skin of 60 rats was treated with dimethylbenzanthracene (DMBA) and mechanical irritation three times a week for 14 weeks. Rats with LSIL were randomly allocated into the experimental group or the control group. The experimental group was treated with focused ultrasound, while the control group received sham treatment.Results After 14 weeks treatment of DMBA combined with mechanical irritation, LSIL were observed in 44 (73.33%) rats, and high-grade squamous intraepithelial lesions (HSIL) were observed in 14 (23.33%) rats. 90.91% (20/22) of rats showed normal pathology and 9.09% (2/22) of rats exhibited LSIL in the experimental group at four weeks after focused ultrasound treatment. 22.73% (5/22) of rats exhibited LSIL, 77.27% (17/22) of rats progressed to HSIL in the control group. Compared with the control-group rats, the levels of HIF-1α, VEGF and mtp53 were significantly decreased in experimental-group rats (p < 0.05).Conclusions These results indicate that DMBA combined with mechanical irritation can induce vulvar squamous intraepithelial lesion in SD rats. Focused ultrasound can treat LSIL safely and effectively, prevent the progression of vulvar lesions, and improve the microenvironment of vulvar tissues by decreasing the localized expression of HIF-1α, VEGF, and mtp53 in rats.https://www.tandfonline.com/doi/10.1080/02656736.2024.2401417Focused ultrasoundsquamous intraepithelial lesionvulvahypoxia-inducible factor 1vascular endothelial growth factormutant p53 |
| spellingShingle | Yijin Fan Xi Wang Yao Liu Huajun Tang Chengzhi Li Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat International Journal of Hyperthermia Focused ultrasound squamous intraepithelial lesion vulva hypoxia-inducible factor 1 vascular endothelial growth factor mutant p53 |
| title | Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat |
| title_full | Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat |
| title_fullStr | Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat |
| title_full_unstemmed | Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat |
| title_short | Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat |
| title_sort | therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat |
| topic | Focused ultrasound squamous intraepithelial lesion vulva hypoxia-inducible factor 1 vascular endothelial growth factor mutant p53 |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2024.2401417 |
| work_keys_str_mv | AT yijinfan therapeuticeffectsoffocusedultrasoundonvulvarsquamousintraepitheliallesionsinrat AT xiwang therapeuticeffectsoffocusedultrasoundonvulvarsquamousintraepitheliallesionsinrat AT yaoliu therapeuticeffectsoffocusedultrasoundonvulvarsquamousintraepitheliallesionsinrat AT huajuntang therapeuticeffectsoffocusedultrasoundonvulvarsquamousintraepitheliallesionsinrat AT chengzhili therapeuticeffectsoffocusedultrasoundonvulvarsquamousintraepitheliallesionsinrat |