Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes
Macrophages are effector cells of the immune system and essential modulators of immune responses. Different functional phenotypes of macrophages with specific roles in the response to stimuli have been described. The C57BL/6 and BALB/c mouse strains tend to selectively display distinct macrophage ac...
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Frontiers Media S.A.
2025-01-01
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| Series: | Experimental Biology and Medicine |
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| Online Access: | https://www.ebm-journal.org/articles/10.3389/ebm.2024.10377/full |
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| author | Carlos M. Restrepo Carlos M. Restrepo Alejandro Llanes Alejandro Llanes Lizzi Herrera Esteban Ellis Esteban Ellis Esteban Ellis Iliana Quintero Patricia L. Fernández Patricia L. Fernández |
| author_facet | Carlos M. Restrepo Carlos M. Restrepo Alejandro Llanes Alejandro Llanes Lizzi Herrera Esteban Ellis Esteban Ellis Esteban Ellis Iliana Quintero Patricia L. Fernández Patricia L. Fernández |
| author_sort | Carlos M. Restrepo |
| collection | DOAJ |
| description | Macrophages are effector cells of the immune system and essential modulators of immune responses. Different functional phenotypes of macrophages with specific roles in the response to stimuli have been described. The C57BL/6 and BALB/c mouse strains tend to selectively display distinct macrophage activation states in response to pathogens, namely, the M1 and M2 phenotypes, respectively. Herein we used RNA-Seq and differential expression analysis to characterize the baseline gene expression pattern of unstimulated resident peritoneal macrophages from C57BL/6 and BALB/c mice. Our aim is to determine if there is a possible predisposition of these mouse strains to any activation phenotype and how this may affect the interpretation of results in studies concerning their interaction with pathogens. We found differences in basal gene expression patterns of BALB/c and C57BL/6 mice, which were further confirmed using RT-PCR for a subset of relevant genes. Despite these differences, our data suggest that baseline gene expression patterns of both mouse strains do not appear to determine by itself a specific macrophage phenotype. |
| format | Article |
| id | doaj-art-e69cbdd645874a90952cbb2bab415fd9 |
| institution | Kabale University |
| issn | 1535-3699 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Experimental Biology and Medicine |
| spelling | doaj-art-e69cbdd645874a90952cbb2bab415fd92025-01-03T17:18:43ZengFrontiers Media S.A.Experimental Biology and Medicine1535-36992025-01-0124910.3389/ebm.2024.1037710377Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypesCarlos M. Restrepo0Carlos M. Restrepo1Alejandro Llanes2Alejandro Llanes3Lizzi Herrera4Esteban Ellis5Esteban Ellis6Esteban Ellis7Iliana Quintero8Patricia L. Fernández9Patricia L. Fernández10Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, PanamaSistema Nacional de Investigación (SNI), Secretaría Nacional de Ciencia Tecnología e Innovación (SENACYT), Panama City, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, PanamaSistema Nacional de Investigación (SNI), Secretaría Nacional de Ciencia Tecnología e Innovación (SENACYT), Panama City, PanamaBioterio, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, PanamaDepartamento de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panama City, PanamaFacultad de Ciencia y Tecnología, Universidad Tecnológica de Panamá, Panama City, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, PanamaSistema Nacional de Investigación (SNI), Secretaría Nacional de Ciencia Tecnología e Innovación (SENACYT), Panama City, PanamaMacrophages are effector cells of the immune system and essential modulators of immune responses. Different functional phenotypes of macrophages with specific roles in the response to stimuli have been described. The C57BL/6 and BALB/c mouse strains tend to selectively display distinct macrophage activation states in response to pathogens, namely, the M1 and M2 phenotypes, respectively. Herein we used RNA-Seq and differential expression analysis to characterize the baseline gene expression pattern of unstimulated resident peritoneal macrophages from C57BL/6 and BALB/c mice. Our aim is to determine if there is a possible predisposition of these mouse strains to any activation phenotype and how this may affect the interpretation of results in studies concerning their interaction with pathogens. We found differences in basal gene expression patterns of BALB/c and C57BL/6 mice, which were further confirmed using RT-PCR for a subset of relevant genes. Despite these differences, our data suggest that baseline gene expression patterns of both mouse strains do not appear to determine by itself a specific macrophage phenotype.https://www.ebm-journal.org/articles/10.3389/ebm.2024.10377/fullmacrophagesRNA-seqBALB/cC57BL/6pathogen |
| spellingShingle | Carlos M. Restrepo Carlos M. Restrepo Alejandro Llanes Alejandro Llanes Lizzi Herrera Esteban Ellis Esteban Ellis Esteban Ellis Iliana Quintero Patricia L. Fernández Patricia L. Fernández Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes Experimental Biology and Medicine macrophages RNA-seq BALB/c C57BL/6 pathogen |
| title | Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes |
| title_full | Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes |
| title_fullStr | Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes |
| title_full_unstemmed | Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes |
| title_short | Baseline gene expression in BALB/c and C57BL/6 peritoneal macrophages influences but does not dictate their functional phenotypes |
| title_sort | baseline gene expression in balb c and c57bl 6 peritoneal macrophages influences but does not dictate their functional phenotypes |
| topic | macrophages RNA-seq BALB/c C57BL/6 pathogen |
| url | https://www.ebm-journal.org/articles/10.3389/ebm.2024.10377/full |
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