The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway
Abstract Background The study aimed to explore whether Miya (MY), a kind of Clostridium butyricum, regulated osteoarthritis (OA) progression through adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway. Methods The OA rats were orally given MY daily for 4 weeks and were intramuscularly...
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BMC
2024-12-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-024-08203-5 |
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| author | Sen Wang Zhengwei Duan Zihua Li Dong Yang Hengli Lu Yiwei Zhang Yuesong Fu Yonghao Guan Guodong Li Feng Qian Tianyang Xu |
| author_facet | Sen Wang Zhengwei Duan Zihua Li Dong Yang Hengli Lu Yiwei Zhang Yuesong Fu Yonghao Guan Guodong Li Feng Qian Tianyang Xu |
| author_sort | Sen Wang |
| collection | DOAJ |
| description | Abstract Background The study aimed to explore whether Miya (MY), a kind of Clostridium butyricum, regulated osteoarthritis (OA) progression through adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway. Methods The OA rats were orally given MY daily for 4 weeks and were intramuscularly injected with AMPK inhibitor once a week for 4 weeks. Hematoxylin eosin (HE) staining was used to observe the histological morphology of the knee joint. The levels of succinate dehydrogenase (SDH) and muscle glycogen (MG) in the tibia muscle of rats were detected by the corresponding kits, as well as the expression of related genes/proteins were assessed by real-time quantitative PCR (RT-qPCR) and western blot. Results HE staining suggested that MY suppressed the symptoms of OA, which was abolished by AMPK inhibitor. Furthermore, the SDH and MG contents in the OA + MY + AMPK inhibitor group were lower than in the OA + MY group. At last, the levels of AMPK, PI3K, AKT1, Ldh, Myod, Chrna1, and Chrnd were notably decreased after AMPK inhibitor treatment, while the levels of Lcad and Mcad were up-regulated by AMPK inhibitor. Furthermore, their protein expression levels detected by western blot were consistent with those from RT-qPCR. Conclusion MY may partially regulate skeletal muscle changes and prevente OA development through the AMPK pathway. |
| format | Article |
| id | doaj-art-e695df1ff5c84647a446d678ed1c2d69 |
| institution | Kabale University |
| issn | 1471-2474 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj-art-e695df1ff5c84647a446d678ed1c2d692025-01-05T12:04:40ZengBMCBMC Musculoskeletal Disorders1471-24742024-12-0125111110.1186/s12891-024-08203-5The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathwaySen Wang0Zhengwei Duan1Zihua Li2Dong Yang3Hengli Lu4Yiwei Zhang5Yuesong Fu6Yonghao Guan7Guodong Li8Feng Qian9Tianyang Xu10Department of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineDepartment of Orthopedics, Bengbu First People’s HospitalDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of MedicineAbstract Background The study aimed to explore whether Miya (MY), a kind of Clostridium butyricum, regulated osteoarthritis (OA) progression through adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway. Methods The OA rats were orally given MY daily for 4 weeks and were intramuscularly injected with AMPK inhibitor once a week for 4 weeks. Hematoxylin eosin (HE) staining was used to observe the histological morphology of the knee joint. The levels of succinate dehydrogenase (SDH) and muscle glycogen (MG) in the tibia muscle of rats were detected by the corresponding kits, as well as the expression of related genes/proteins were assessed by real-time quantitative PCR (RT-qPCR) and western blot. Results HE staining suggested that MY suppressed the symptoms of OA, which was abolished by AMPK inhibitor. Furthermore, the SDH and MG contents in the OA + MY + AMPK inhibitor group were lower than in the OA + MY group. At last, the levels of AMPK, PI3K, AKT1, Ldh, Myod, Chrna1, and Chrnd were notably decreased after AMPK inhibitor treatment, while the levels of Lcad and Mcad were up-regulated by AMPK inhibitor. Furthermore, their protein expression levels detected by western blot were consistent with those from RT-qPCR. Conclusion MY may partially regulate skeletal muscle changes and prevente OA development through the AMPK pathway.https://doi.org/10.1186/s12891-024-08203-5MiyaOsteoarthritisAMPKGut microbiota |
| spellingShingle | Sen Wang Zhengwei Duan Zihua Li Dong Yang Hengli Lu Yiwei Zhang Yuesong Fu Yonghao Guan Guodong Li Feng Qian Tianyang Xu The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway BMC Musculoskeletal Disorders Miya Osteoarthritis AMPK Gut microbiota |
| title | The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway |
| title_full | The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway |
| title_fullStr | The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway |
| title_full_unstemmed | The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway |
| title_short | The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway |
| title_sort | effect of miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through ampk pathway |
| topic | Miya Osteoarthritis AMPK Gut microbiota |
| url | https://doi.org/10.1186/s12891-024-08203-5 |
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