Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs
Background: Recent studies have discussed the preferred activity of synbiotics, which are a combination of probiotics and prebiotics. This study formulates a novel synbiotic that includes Chinese traditional herbs and evaluates its immunomodulatory function. Purpose: This study aims to systematicall...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324005349 |
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| author | Deshan Ning Yiru Huang Yulong Zhou Hongwei Zhao Wenlei Nie Yu Zheng Xiaojun Huang |
| author_facet | Deshan Ning Yiru Huang Yulong Zhou Hongwei Zhao Wenlei Nie Yu Zheng Xiaojun Huang |
| author_sort | Deshan Ning |
| collection | DOAJ |
| description | Background: Recent studies have discussed the preferred activity of synbiotics, which are a combination of probiotics and prebiotics. This study formulates a novel synbiotic that includes Chinese traditional herbs and evaluates its immunomodulatory function. Purpose: This study aims to systematically investigate the effects of synbiotics on immune function using a cyclophosphamide-induced immunosuppressed mouse model. Study design: A literature-based approach was used to selectively identify medicinal herbs with immunomodulatory properties. Commercially available probiotics were used to investigate the immunomodulatory functions and potential mechanisms associated with the combination of Chinese medicinal herbs and prebiotics as carbon substrates. Methods: The immunomodulatory effect of the synbiotic will be assessed by measuring indicators such as body weight, organ index, immunoglobulins, cytokines, splenic lymphocyte proliferation status, NK cell cytotoxicity and gut microbiota. Results: Our results demonstrated that the synbiotic significantly restored the decreased thymus index and the increased spleen index induced by immune deficiency in mice. Among them, the formula of the synbiotics significantly improves the thymus organ index of immune organs from 0.068 to 0.126, which is also significantly superior compared to other single components. Additionally, it significantly enhanced the proliferative capacity of splenic lymphocytes and the cytotoxic activity of natural killer (NK) cells in immune-deficient mice. Furthermore, the synbiotics enhanced the richness and diversity of the gut microbiota, rectifying the dysbiosis in gut microbial composition caused by immune deficiency. It decreased the relative abundance of Firmicutes from 67.41 % to 44.06 %, while increasing the relative abundance of Bacteroidota from 30.42 % to 53.77 %. It restored the disrupted gut microbial structure induced by cyclophosphamide damage, thereby ameliorating the disrupted intestinal microbial community associated with immune deficiency. Conclusion: The study demonstrated that the synbiotic was capable of modulating immune function, as evidenced by an increase in organ index, cellular immunity, and the ability to modulate intestinal flora disorders. Furthermore, the synbiotic demonstrated superior performance compared to other single components. |
| format | Article |
| id | doaj-art-e66eec633a2e4c10934e4e2f7a35f721 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-e66eec633a2e4c10934e4e2f7a35f7212024-12-16T05:38:06ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-0118101497Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbsDeshan Ning0Yiru Huang1Yulong Zhou2Hongwei Zhao3Wenlei Nie4Yu Zheng5Xiaojun Huang6Infinitus (China) Co., Ltd. R & D Center, 510653, Guangzhou, Guangdong, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, ChinaInfinitus (China) Co., Ltd. R & D Center, 510653, Guangzhou, Guangdong, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, China; Corresponding author. 235 Nanjing East Road, Nanchang, 330047, China.Background: Recent studies have discussed the preferred activity of synbiotics, which are a combination of probiotics and prebiotics. This study formulates a novel synbiotic that includes Chinese traditional herbs and evaluates its immunomodulatory function. Purpose: This study aims to systematically investigate the effects of synbiotics on immune function using a cyclophosphamide-induced immunosuppressed mouse model. Study design: A literature-based approach was used to selectively identify medicinal herbs with immunomodulatory properties. Commercially available probiotics were used to investigate the immunomodulatory functions and potential mechanisms associated with the combination of Chinese medicinal herbs and prebiotics as carbon substrates. Methods: The immunomodulatory effect of the synbiotic will be assessed by measuring indicators such as body weight, organ index, immunoglobulins, cytokines, splenic lymphocyte proliferation status, NK cell cytotoxicity and gut microbiota. Results: Our results demonstrated that the synbiotic significantly restored the decreased thymus index and the increased spleen index induced by immune deficiency in mice. Among them, the formula of the synbiotics significantly improves the thymus organ index of immune organs from 0.068 to 0.126, which is also significantly superior compared to other single components. Additionally, it significantly enhanced the proliferative capacity of splenic lymphocytes and the cytotoxic activity of natural killer (NK) cells in immune-deficient mice. Furthermore, the synbiotics enhanced the richness and diversity of the gut microbiota, rectifying the dysbiosis in gut microbial composition caused by immune deficiency. It decreased the relative abundance of Firmicutes from 67.41 % to 44.06 %, while increasing the relative abundance of Bacteroidota from 30.42 % to 53.77 %. It restored the disrupted gut microbial structure induced by cyclophosphamide damage, thereby ameliorating the disrupted intestinal microbial community associated with immune deficiency. Conclusion: The study demonstrated that the synbiotic was capable of modulating immune function, as evidenced by an increase in organ index, cellular immunity, and the ability to modulate intestinal flora disorders. Furthermore, the synbiotic demonstrated superior performance compared to other single components.http://www.sciencedirect.com/science/article/pii/S2666154324005349Traditional Chinese medicineSynbioticProbioticsImmunomodulatory |
| spellingShingle | Deshan Ning Yiru Huang Yulong Zhou Hongwei Zhao Wenlei Nie Yu Zheng Xiaojun Huang Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs Journal of Agriculture and Food Research Traditional Chinese medicine Synbiotic Probiotics Immunomodulatory |
| title | Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs |
| title_full | Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs |
| title_fullStr | Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs |
| title_full_unstemmed | Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs |
| title_short | Evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs |
| title_sort | evaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic prebiotic chinese traditional herbs |
| topic | Traditional Chinese medicine Synbiotic Probiotics Immunomodulatory |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324005349 |
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