Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model
Nanoplastics (NPs) are emerging environmental pollutants that pose significant health risks, particularly due to their accumulation in the gastrointestinal tract. This study evaluates the ability of 88 native probiotic Lactobacillus strains to remove polystyrene nanoplastics (PS-NPs) in an in-vitro...
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Elsevier
2025-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325009443 |
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| author | Nayereh Rezaie Rahimi Mohammad Hoseini Hasan Hashemi Abooalfazl Azhdarpoor Hamid Gholami Pourbadie Mansooreh Dehghani Mahdi Rohani |
| author_facet | Nayereh Rezaie Rahimi Mohammad Hoseini Hasan Hashemi Abooalfazl Azhdarpoor Hamid Gholami Pourbadie Mansooreh Dehghani Mahdi Rohani |
| author_sort | Nayereh Rezaie Rahimi |
| collection | DOAJ |
| description | Nanoplastics (NPs) are emerging environmental pollutants that pose significant health risks, particularly due to their accumulation in the gastrointestinal tract. This study evaluates the ability of 88 native probiotic Lactobacillus strains to remove polystyrene nanoplastics (PS-NPs) in an in-vitro model. Three resistant strains L. plantarum RP13, RP134, and RP225—were selected based on disk diffusion screening. A cocktail of these strains demonstrated high percentages of NP binding: 77 % ± 2.5 at 0.1 mg/mL, 76 %± 2.1 at 0.2 mg/mL, and 67 %± 3.0 at 0.4 mg/mL. Among individual strains, RP13 exhibited the highest removal capacity. Fluorescence and electron microscopy confirmed PS-NP adhesion to bacterial surfaces. Zeta potential measurements showed a shift from –30.32 mV to –21.87 mV after bacterial exposure, indicating surface interaction. Cytotoxicity assays on HT-29 cells revealed dose-dependent toxicity of PS-NPs, which was significantly reduced by treatment with the Lactobacillus cocktail most notably at concentrations between 62.5 and 15.62 µg/mL. These promising in-vitro results suggest that our native probiotic Lactobacillus strains may offer a sustainable strategy for mitigating nanoplastic toxicity in the gastrointestinal tract; however, validation through in-vivo studies is essential to confirm their therapeutic potential and translational relevance. |
| format | Article |
| id | doaj-art-e65a6e707d494291a278376d0497dd4e |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e65a6e707d494291a278376d0497dd4e2025-08-20T04:02:31ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211859910.1016/j.ecoenv.2025.118599Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro ModelNayereh Rezaie Rahimi0Mohammad Hoseini1Hasan Hashemi2Abooalfazl Azhdarpoor3Hamid Gholami Pourbadie4Mansooreh Dehghani5Mahdi Rohani6Department of Environmental Health Engineering, School of Public Health, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Bacteriology, Pasteur Institute of Iran, Tehran, IranDepartment of Environmental Health Engineering, School of Public Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Environmental Health Engineering, School of Public Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Environmental Health Engineering, School of Public Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, IranDepartment of Environmental Health Engineering, School of Public Health, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding authors.Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran; Corresponding authors.Nanoplastics (NPs) are emerging environmental pollutants that pose significant health risks, particularly due to their accumulation in the gastrointestinal tract. This study evaluates the ability of 88 native probiotic Lactobacillus strains to remove polystyrene nanoplastics (PS-NPs) in an in-vitro model. Three resistant strains L. plantarum RP13, RP134, and RP225—were selected based on disk diffusion screening. A cocktail of these strains demonstrated high percentages of NP binding: 77 % ± 2.5 at 0.1 mg/mL, 76 %± 2.1 at 0.2 mg/mL, and 67 %± 3.0 at 0.4 mg/mL. Among individual strains, RP13 exhibited the highest removal capacity. Fluorescence and electron microscopy confirmed PS-NP adhesion to bacterial surfaces. Zeta potential measurements showed a shift from –30.32 mV to –21.87 mV after bacterial exposure, indicating surface interaction. Cytotoxicity assays on HT-29 cells revealed dose-dependent toxicity of PS-NPs, which was significantly reduced by treatment with the Lactobacillus cocktail most notably at concentrations between 62.5 and 15.62 µg/mL. These promising in-vitro results suggest that our native probiotic Lactobacillus strains may offer a sustainable strategy for mitigating nanoplastic toxicity in the gastrointestinal tract; however, validation through in-vivo studies is essential to confirm their therapeutic potential and translational relevance.http://www.sciencedirect.com/science/article/pii/S0147651325009443MicroplasticsPolystyrene NanoplasticLactobacillusProbioticsMTT AssayHuman Colorectal Adenocarcinoma Cell Line |
| spellingShingle | Nayereh Rezaie Rahimi Mohammad Hoseini Hasan Hashemi Abooalfazl Azhdarpoor Hamid Gholami Pourbadie Mansooreh Dehghani Mahdi Rohani Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model Ecotoxicology and Environmental Safety Microplastics Polystyrene Nanoplastic Lactobacillus Probiotics MTT Assay Human Colorectal Adenocarcinoma Cell Line |
| title | Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model |
| title_full | Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model |
| title_fullStr | Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model |
| title_full_unstemmed | Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model |
| title_short | Determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in-vitro Model |
| title_sort | determination of the ability of native potential probiotic lactobacillus strains in nanoplastic bioremoval in an in vitro model |
| topic | Microplastics Polystyrene Nanoplastic Lactobacillus Probiotics MTT Assay Human Colorectal Adenocarcinoma Cell Line |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325009443 |
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