Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64
The biological activities of ethyl acetate extracts of Lasiodiplodia pseudotheobromae IBRL OS-64 were assessed in the present study. Antioxidant activity was measured using the DPPH scavenging assay, antibacterial activity via broth microdilution, and total phenolic content using the Folin-Ciocalte...
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Bogor Agricultural University
2024-12-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/55561 |
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| author | Mohd Taufiq Mat Jalil Nurul Aili Zakaria Mohd Fakharul Zaman Raja Yahya Sharifah Aminah Syed Mohamad Darah Ibrahim |
| author_facet | Mohd Taufiq Mat Jalil Nurul Aili Zakaria Mohd Fakharul Zaman Raja Yahya Sharifah Aminah Syed Mohamad Darah Ibrahim |
| author_sort | Mohd Taufiq Mat Jalil |
| collection | DOAJ |
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The biological activities of ethyl acetate extracts of Lasiodiplodia pseudotheobromae IBRL OS-64 were assessed in the present study. Antioxidant activity was measured using the DPPH scavenging assay, antibacterial activity via broth microdilution, and total phenolic content using the Folin-Ciocalteu method. In vivo toxicity was determined using a brine shrimp lethality assay. The fraction extract exhibited the highest antioxidant activity, with an EC50 of 441.6 µg/ml, surpassing the crude extract's. The EEELP showed a relatively low phenolic content of 13.273 µg GAE/mg extract. Regarding antibacterial activity, both crude and fraction extracts demonstrated MIC values ranging from 62.5 to 250 µg/ml against Gram-positive bacteria, while MBC values ranged from 125 to 500 µg/ml for both extracts. The MIC values for crude and fraction extracts against Gram-negative bacteria were 250-500 µg/ml, whilst the MBC values for both crude and fraction extracts were in the range of 500–2,000 µg/ml. The findings also revealed that Gram-positive bacteria were more susceptible to both extracts (crude and fraction) than Gram-negative bacteria. The crude extract exhibited a non-cytotoxic effect with an LC50 value of 2054.88 µg/ml for acute exposure and a low cytotoxic effect with an LC50 value of 199.69 µg/ml for chronic exposure. Meanwhile, the fraction extract demonstrated a non-cytotoxic effect for both acute and chronic with an LC50 value of 5744.14 µg/ml and 1035.94 µg/ml, respectively. Therefore, the present study suggests that the fraction extract could be an effective pharmaceutical agent and safe for drug development due to its low toxicity.
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| format | Article |
| id | doaj-art-e648b70c887d4915b867c2894e6f64f9 |
| institution | Kabale University |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bogor Agricultural University |
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| series | Hayati Journal of Biosciences |
| spelling | doaj-art-e648b70c887d4915b867c2894e6f64f92025-01-08T09:00:41ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942024-12-0132210.4308/hjb.32.2.445-458Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64Mohd Taufiq Mat Jalil0Nurul Aili Zakaria1Mohd Fakharul Zaman Raja Yahya2Sharifah Aminah Syed Mohamad3Darah Ibrahim4School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450, Malaysia. Molecular Microbial Pathogenicity Research Group, Pharmaceutical and Life Sciences CoRe, Universiti Teknologi MARA, Shah Alam, Selangor 40450, MalaysiaSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450, MalaysiaSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450, Malaysia. Molecular Microbial Pathogenicity Research Group, Pharmaceutical and Life Sciences CoRe, Universiti Teknologi MARA, Shah Alam, Selangor 40450, MalaysiaSchool of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450, MalaysiaIndustrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, 11800, Malaysia The biological activities of ethyl acetate extracts of Lasiodiplodia pseudotheobromae IBRL OS-64 were assessed in the present study. Antioxidant activity was measured using the DPPH scavenging assay, antibacterial activity via broth microdilution, and total phenolic content using the Folin-Ciocalteu method. In vivo toxicity was determined using a brine shrimp lethality assay. The fraction extract exhibited the highest antioxidant activity, with an EC50 of 441.6 µg/ml, surpassing the crude extract's. The EEELP showed a relatively low phenolic content of 13.273 µg GAE/mg extract. Regarding antibacterial activity, both crude and fraction extracts demonstrated MIC values ranging from 62.5 to 250 µg/ml against Gram-positive bacteria, while MBC values ranged from 125 to 500 µg/ml for both extracts. The MIC values for crude and fraction extracts against Gram-negative bacteria were 250-500 µg/ml, whilst the MBC values for both crude and fraction extracts were in the range of 500–2,000 µg/ml. The findings also revealed that Gram-positive bacteria were more susceptible to both extracts (crude and fraction) than Gram-negative bacteria. The crude extract exhibited a non-cytotoxic effect with an LC50 value of 2054.88 µg/ml for acute exposure and a low cytotoxic effect with an LC50 value of 199.69 µg/ml for chronic exposure. Meanwhile, the fraction extract demonstrated a non-cytotoxic effect for both acute and chronic with an LC50 value of 5744.14 µg/ml and 1035.94 µg/ml, respectively. Therefore, the present study suggests that the fraction extract could be an effective pharmaceutical agent and safe for drug development due to its low toxicity. https://journal.ipb.ac.id/index.php/hayati/article/view/55561 |
| spellingShingle | Mohd Taufiq Mat Jalil Nurul Aili Zakaria Mohd Fakharul Zaman Raja Yahya Sharifah Aminah Syed Mohamad Darah Ibrahim Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 Hayati Journal of Biosciences |
| title | Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 |
| title_full | Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 |
| title_fullStr | Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 |
| title_full_unstemmed | Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 |
| title_short | Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 |
| title_sort | assessment of biological activity total phenolic content and cytotoxicity of ethyl acetate extracts from an endophytic fungus lasiodiplodia pseudotheobromae ibrl os 64 |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/55561 |
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