Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus
The promoter activity differences of two manganese-containing superoxide dismutase (MnSOD) genes sodA1 and sodA2 in Bacillus cereus 905 were assayed in vivo by use of lacZ reporter. The results showed that sodA1 and sodA2 were transcripted constitutively during the bacterial growth. The promoter act...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2012-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.001 |
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| author | LIU Juan WANG Song ZHANG Li-qin WANG Yong-jun WANG Qi |
| author_facet | LIU Juan WANG Song ZHANG Li-qin WANG Yong-jun WANG Qi |
| author_sort | LIU Juan |
| collection | DOAJ |
| description | The promoter activity differences of two manganese-containing superoxide dismutase (MnSOD) genes sodA1 and sodA2 in Bacillus cereus 905 were assayed in vivo by use of lacZ reporter. The results showed that sodA1 and sodA2 were transcripted constitutively during the bacterial growth. The promoter activity of sodA1 were 3-4 times higher than that of sodA2. Compared with wild type, both sodA1 and sodA2 were induced in a MnSOD-deficient strain KOS, which suggested some unknown regularity for sodA1 and sodA2 transcriptions in response to oxidative stress. Additionally, bacterial blue colonies caused by the high β-galactosidase activity under the sodA1-lacZ reporter indicated that sodA1-lacZ reporter was feasible to label B. cereus. |
| format | Article |
| id | doaj-art-e5c9b73b05b7487e8d2e7203c8ee7f97 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2012-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-e5c9b73b05b7487e8d2e7203c8ee7f972025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552012-05-013823724210.3785/j.issn.1008-9209.2012.03.00110089209Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereusLIU JuanWANG SongZHANG Li-qinWANG Yong-junWANG QiThe promoter activity differences of two manganese-containing superoxide dismutase (MnSOD) genes sodA1 and sodA2 in Bacillus cereus 905 were assayed in vivo by use of lacZ reporter. The results showed that sodA1 and sodA2 were transcripted constitutively during the bacterial growth. The promoter activity of sodA1 were 3-4 times higher than that of sodA2. Compared with wild type, both sodA1 and sodA2 were induced in a MnSOD-deficient strain KOS, which suggested some unknown regularity for sodA1 and sodA2 transcriptions in response to oxidative stress. Additionally, bacterial blue colonies caused by the high β-galactosidase activity under the sodA1-lacZ reporter indicated that sodA1-lacZ reporter was feasible to label B. cereus.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.001<italic>Bacillus cereus</italic>superoxide dismutasetranscriptional differenceLacZ labeling |
| spellingShingle | LIU Juan WANG Song ZHANG Li-qin WANG Yong-jun WANG Qi Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus 浙江大学学报. 农业与生命科学版 <italic>Bacillus cereus</italic> superoxide dismutase transcriptional difference LacZ labeling |
| title | Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus |
| title_full | Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus |
| title_fullStr | Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus |
| title_full_unstemmed | Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus |
| title_short | Transcriptional differences between two distinct manganese-containing superoxide dismutase genes in Bacillus cereus |
| title_sort | transcriptional differences between two distinct manganese containing superoxide dismutase genes in bacillus cereus |
| topic | <italic>Bacillus cereus</italic> superoxide dismutase transcriptional difference LacZ labeling |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.001 |
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