miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells
Abstract miRNA, short non-coding RNA, are rapidly emerging as important regulators in cell homeostasis, as well as potential players in cellular degeneration. The latter has led to interest in them as both biomarkers and as potential therapeutics. Retinal ganglion cells (RGC), whose axons connect th...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-83381-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559473866407936 |
|---|---|
| author | Maryam Esmaeili Daniel A. Smith Ben Mead |
| author_facet | Maryam Esmaeili Daniel A. Smith Ben Mead |
| author_sort | Maryam Esmaeili |
| collection | DOAJ |
| description | Abstract miRNA, short non-coding RNA, are rapidly emerging as important regulators in cell homeostasis, as well as potential players in cellular degeneration. The latter has led to interest in them as both biomarkers and as potential therapeutics. Retinal ganglion cells (RGC), whose axons connect the eye to the brain, are central nervous system cells of great interest, yet their study is largely restricted to animals due to the difficulty in obtaining healthy human RGC. Using a CRISPR/Cas9-based reporter embryonic stem cell line, human RGC were generated and their miRNA profile characterized using NanoString miRNA assays. We identified a variety of retinal specific miRNA upregulated in ESC-derived RGC, with half of the most abundant miRNA also detectable in purified rat RGC. Several miRNA were however identified to be unique to RGC from human. The findings show which miRNA are abundant in RGC and the limited congruence with animal derived RGC. These data could be used to understand miRNA’s role in RGC function, as well as potential biomarkers or therapies in retinal diseases involving RGC degeneration. |
| format | Article |
| id | doaj-art-e5b3d2c30aad43d99952fa9a3e335cb1 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e5b3d2c30aad43d99952fa9a3e335cb12025-01-05T12:30:15ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-83381-9miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cellsMaryam Esmaeili0Daniel A. Smith1Ben Mead2School of Optometry and Vision Sciences, Cardiff UniversityWales Kidney Research Unit, School of Medicine, Cardiff UniversitySchool of Optometry and Vision Sciences, Cardiff UniversityAbstract miRNA, short non-coding RNA, are rapidly emerging as important regulators in cell homeostasis, as well as potential players in cellular degeneration. The latter has led to interest in them as both biomarkers and as potential therapeutics. Retinal ganglion cells (RGC), whose axons connect the eye to the brain, are central nervous system cells of great interest, yet their study is largely restricted to animals due to the difficulty in obtaining healthy human RGC. Using a CRISPR/Cas9-based reporter embryonic stem cell line, human RGC were generated and their miRNA profile characterized using NanoString miRNA assays. We identified a variety of retinal specific miRNA upregulated in ESC-derived RGC, with half of the most abundant miRNA also detectable in purified rat RGC. Several miRNA were however identified to be unique to RGC from human. The findings show which miRNA are abundant in RGC and the limited congruence with animal derived RGC. These data could be used to understand miRNA’s role in RGC function, as well as potential biomarkers or therapies in retinal diseases involving RGC degeneration.https://doi.org/10.1038/s41598-024-83381-9 |
| spellingShingle | Maryam Esmaeili Daniel A. Smith Ben Mead miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells Scientific Reports |
| title | miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells |
| title_full | miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells |
| title_fullStr | miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells |
| title_full_unstemmed | miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells |
| title_short | miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells |
| title_sort | mirna changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells |
| url | https://doi.org/10.1038/s41598-024-83381-9 |
| work_keys_str_mv | AT maryamesmaeili mirnachangesassociatedwithdifferentiationofhumanembryonicstemcellsintohumanretinalganglioncells AT danielasmith mirnachangesassociatedwithdifferentiationofhumanembryonicstemcellsintohumanretinalganglioncells AT benmead mirnachangesassociatedwithdifferentiationofhumanembryonicstemcellsintohumanretinalganglioncells |