Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles
Formation of unilamellae of fully hydrated dipalmitoylphosphatidylcholine (DPPC) was induced by a horn sonicator from multilamellar vesicles and followed by time-resolved synchrotron small angle X-ray scattering and direct visual morphological investigations by the means of transmission electron-mic...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S135041772400436X |
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| author | Attila Bóta Heinz Amenitsch András Wacha |
| author_facet | Attila Bóta Heinz Amenitsch András Wacha |
| author_sort | Attila Bóta |
| collection | DOAJ |
| description | Formation of unilamellae of fully hydrated dipalmitoylphosphatidylcholine (DPPC) was induced by a horn sonicator from multilamellar vesicles and followed by time-resolved synchrotron small angle X-ray scattering and direct visual morphological investigations by the means of transmission electron-microscopy combined with freeze-fracture. Without incubation the ultrasonication causes continuous increasing in temperature and transformation from the gel to rippled gel structures, then reaching the main transition, the formfactor of unilamellar structure appeared. The ultrasonication resulted in different layer formations at the characteristic temperatures of the gel (20 °C), rippled gel (38 °C), and liquid crystalline (45 °C) phases of the system. At 20 °C irregular stacks of multi and oligolamellar lamellae were shaped even after three hours of ultrasonication. At 38 and 45 °C the ultrasound induced dominantly unilamellar vesicles (ULVs) in a short time (10 and 3 – 5 min, respectively, under typical ultrasound treatments in the general laboratory practice). After the end of the ultrasonication, irregular layer formations with defects structures increased when the temperature of sonication was above the chain melting temperature of the hydrated DPPC system, underlining the importance of optimized sonication processes. |
| format | Article |
| id | doaj-art-e5aed7ac0f0e4d8b9d58c55785d947a1 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-e5aed7ac0f0e4d8b9d58c55785d947a12025-01-11T06:38:46ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107187Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesiclesAttila Bóta0Heinz Amenitsch1András Wacha2Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Magyar tudósok körútja 2, 1117 Budapest, Hungary; Corresponding author.Austrian SAXS beamline@ELETTRA, Italy and Inorganic Chemistry, Graz University of Technology, Are Science Park 34149 Basovizza TS, Trieste, Stremayrgasse 9, 8010 Graz, AustriaResearch Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Magyar tudósok körútja 2, 1117 Budapest, HungaryFormation of unilamellae of fully hydrated dipalmitoylphosphatidylcholine (DPPC) was induced by a horn sonicator from multilamellar vesicles and followed by time-resolved synchrotron small angle X-ray scattering and direct visual morphological investigations by the means of transmission electron-microscopy combined with freeze-fracture. Without incubation the ultrasonication causes continuous increasing in temperature and transformation from the gel to rippled gel structures, then reaching the main transition, the formfactor of unilamellar structure appeared. The ultrasonication resulted in different layer formations at the characteristic temperatures of the gel (20 °C), rippled gel (38 °C), and liquid crystalline (45 °C) phases of the system. At 20 °C irregular stacks of multi and oligolamellar lamellae were shaped even after three hours of ultrasonication. At 38 and 45 °C the ultrasound induced dominantly unilamellar vesicles (ULVs) in a short time (10 and 3 – 5 min, respectively, under typical ultrasound treatments in the general laboratory practice). After the end of the ultrasonication, irregular layer formations with defects structures increased when the temperature of sonication was above the chain melting temperature of the hydrated DPPC system, underlining the importance of optimized sonication processes.http://www.sciencedirect.com/science/article/pii/S135041772400436XUltrasonicationUltrasound induced unilamellar vesiclePhase-transitionBilayered phospholipid fragmentMLV to ULV transformationTime-resolved in situ SAXS |
| spellingShingle | Attila Bóta Heinz Amenitsch András Wacha Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles Ultrasonics Sonochemistry Ultrasonication Ultrasound induced unilamellar vesicle Phase-transition Bilayered phospholipid fragment MLV to ULV transformation Time-resolved in situ SAXS |
| title | Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles |
| title_full | Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles |
| title_fullStr | Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles |
| title_full_unstemmed | Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles |
| title_short | Lamellarity of ultrasound assisted formations of dipalmitoyl-lecithin vesicles |
| title_sort | lamellarity of ultrasound assisted formations of dipalmitoyl lecithin vesicles |
| topic | Ultrasonication Ultrasound induced unilamellar vesicle Phase-transition Bilayered phospholipid fragment MLV to ULV transformation Time-resolved in situ SAXS |
| url | http://www.sciencedirect.com/science/article/pii/S135041772400436X |
| work_keys_str_mv | AT attilabota lamellarityofultrasoundassistedformationsofdipalmitoyllecithinvesicles AT heinzamenitsch lamellarityofultrasoundassistedformationsofdipalmitoyllecithinvesicles AT andraswacha lamellarityofultrasoundassistedformationsofdipalmitoyllecithinvesicles |