Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells
The insect silk protects the larvae that produce it. This implies that it may contain biologically active substances that can be used in medicine. In this paper, the cytotoxic and proapoptotic potential of Indian meal moth (Plodia interpunctella) silk extract was examined after 72 h of exposure to t...
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| Format: | Article |
| Language: | English |
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University of Kragujevac, Faculty of Science
2024-01-01
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| Series: | Kragujevac Journal of Science |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362402039N.pdf |
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| author | Nikodijević Danijela Milutinović Milena Radenković Nikola Blagojević Stefan Vasiljević Anđela Jurišić Vladimir Predojević Dragana Vukajlović Filip Pešić Snežana |
| author_facet | Nikodijević Danijela Milutinović Milena Radenković Nikola Blagojević Stefan Vasiljević Anđela Jurišić Vladimir Predojević Dragana Vukajlović Filip Pešić Snežana |
| author_sort | Nikodijević Danijela |
| collection | DOAJ |
| description | The insect silk protects the larvae that produce it. This implies that it may contain biologically active substances that can be used in medicine. In this paper, the cytotoxic and proapoptotic potential of Indian meal moth (Plodia interpunctella) silk extract was examined after 72 h of exposure to the SW-480 human colorectal adenocarcinoma cell line. Apoptosis was examined by monitoring the cell morphological changes by fluorescent staining, acridine orange/ethidium bromide, and DAPI, as well as by flow cytometry. The results showed that silk extract has a very good cytotoxic and proapoptotic activity. The selective effect of silk indicates the presence of a high level of biocompatibility with normal human cells. Based on the results of increased gene expression for caspase 8 and 9 in silk extract-treated cells compared to control, apoptosis was induced by a caspase-dependent pathway. |
| format | Article |
| id | doaj-art-e59e3c7c87454ce0afc1fcd20e4a1faa |
| institution | Kabale University |
| issn | 1450-9636 2466-5509 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Kragujevac, Faculty of Science |
| record_format | Article |
| series | Kragujevac Journal of Science |
| spelling | doaj-art-e59e3c7c87454ce0afc1fcd20e4a1faa2025-01-08T16:26:53ZengUniversity of Kragujevac, Faculty of ScienceKragujevac Journal of Science1450-96362466-55092024-01-01462394810.5937/KgJSci2400015N1450-96362402039NSilk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cellsNikodijević Danijela0https://orcid.org/0000-0002-6453-0313Milutinović Milena1https://orcid.org/0000-0002-5075-5650Radenković Nikola2https://orcid.org/0000-0002-8458-2022Blagojević Stefan3https://orcid.org/0000-0002-5640-6267Vasiljević Anđela4Jurišić Vladimir5https://orcid.org/0000-0001-6525-128XPredojević Dragana6https://orcid.org/0000-0002-9374-0563Vukajlović Filip7https://orcid.org/0000-0003-4879-0171Pešić Snežana8https://orcid.org/0000-0001-7762-1372University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Science, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaThe insect silk protects the larvae that produce it. This implies that it may contain biologically active substances that can be used in medicine. In this paper, the cytotoxic and proapoptotic potential of Indian meal moth (Plodia interpunctella) silk extract was examined after 72 h of exposure to the SW-480 human colorectal adenocarcinoma cell line. Apoptosis was examined by monitoring the cell morphological changes by fluorescent staining, acridine orange/ethidium bromide, and DAPI, as well as by flow cytometry. The results showed that silk extract has a very good cytotoxic and proapoptotic activity. The selective effect of silk indicates the presence of a high level of biocompatibility with normal human cells. Based on the results of increased gene expression for caspase 8 and 9 in silk extract-treated cells compared to control, apoptosis was induced by a caspase-dependent pathway.https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362402039N.pdfcolon cancerplodia interpunctellaapoptosiscaspasesbiocompatibility |
| spellingShingle | Nikodijević Danijela Milutinović Milena Radenković Nikola Blagojević Stefan Vasiljević Anđela Jurišić Vladimir Predojević Dragana Vukajlović Filip Pešić Snežana Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells Kragujevac Journal of Science colon cancer plodia interpunctella apoptosis caspases biocompatibility |
| title | Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells |
| title_full | Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells |
| title_fullStr | Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells |
| title_full_unstemmed | Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells |
| title_short | Silk of the Indian meal moth Plodia interpunctella (Hübner, 1813) affects the human colon cancer cells |
| title_sort | silk of the indian meal moth plodia interpunctella hubner 1813 affects the human colon cancer cells |
| topic | colon cancer plodia interpunctella apoptosis caspases biocompatibility |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362402039N.pdf |
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