Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant

This study explores C. edulis, a plant indigenous to the Cholistan desert, locally known as Pimpa or Seetu, traditionally consumed as a vegetable. Our research aimed to comprehensively analyze its phytochemical constituents, and evaluate its antibacterial, antioxidant, antiviral, anti-inflammatory,...

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Main Authors: Bushra Gillani, Sarah Tariq, Mirza Imran Shahzad, Tatheer Fatima, Marcello Locatelli, Xinxia Cai, Adnan Noor Shah, Ajaz Ahmad
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Journal of King Saud University: Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S1018364724004312
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author Bushra Gillani
Sarah Tariq
Mirza Imran Shahzad
Tatheer Fatima
Marcello Locatelli
Xinxia Cai
Adnan Noor Shah
Ajaz Ahmad
author_facet Bushra Gillani
Sarah Tariq
Mirza Imran Shahzad
Tatheer Fatima
Marcello Locatelli
Xinxia Cai
Adnan Noor Shah
Ajaz Ahmad
author_sort Bushra Gillani
collection DOAJ
description This study explores C. edulis, a plant indigenous to the Cholistan desert, locally known as Pimpa or Seetu, traditionally consumed as a vegetable. Our research aimed to comprehensively analyze its phytochemical constituents, and evaluate its antibacterial, antioxidant, antiviral, anti-inflammatory, antidiabetic, and antipyretic potentials. Utilizing a range of extracts including methanol (MtOH), ethanol (EtOH), ethyl acetate (EA), n-hexane (n-hex), dichloromethane (DCM), and aqueous (Aq), for effective extraction of phytochemicals from C. edulis. Standard biochemical assays and High-Performance Liquid Chromatography-Photodiode Array (HPLC-PDA) were used for analysis of phenolic compounds. Antibacterial effect(s) were confirmed through disc diffusion method and min inhibitory concentrations (MICs). The antioxidant activity was assessed through the Ferric Reducing Antioxidant Power (FRAP) assay and the DPPH radical scavenging method. In vivo antiviral potential was assessed through Hemagglutination (HA) test. Anti-inflammatory, antidiabetic, and antipyretic activities were performed on female albino rats using carrageenan, alloxan monohydrate and yeast-induced methods, respectively. Statistical analysis was done using standard one way ANOVA.Our findings revealed a rich diversity of phenolic compounds and the presence of proteins, alkaloids, and carbohydrates in C. edulis. MtOH and n-hex extracts demonstrated deep antiviral activity against various viral strains. In vivo toxicology studies indicated no significant toxicity at doses up to 5 g/kg. The DCM extract has shown notable anti-inflammatory effects, and EA extract was leading in antipyretic activity. All extracts, except MtOH, exhibited antidiabetic properties.In conclusion, C. edulis emerges not only as a valuable nutritional source but also as a potent alternative medicinal resource, offering wide range of therapeutic benefits.
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spelling doaj-art-e59df1c43eb14c848aebb75a7b20d0fc2024-11-13T04:29:25ZengElsevierJournal of King Saud University: Science1018-36472024-12-013611103519Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plantBushra Gillani0Sarah Tariq1Mirza Imran Shahzad2Tatheer Fatima3Marcello Locatelli4Xinxia Cai5Adnan Noor Shah6Ajaz Ahmad7Department of Biochemistry & Molecular Biology, IBBB, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Biochemistry & Molecular Biology, IBBB, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Biochemistry & Molecular Biology, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan; Corresponding authors at: Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 332900, China (A.N. Shah).Department of Biochemistry & Molecular Biology, IBBB, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 332900, China; Department of Agricultural Engineering, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, Punjab, Pakistan; Corresponding authors at: Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 332900, China (A.N. Shah).Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaThis study explores C. edulis, a plant indigenous to the Cholistan desert, locally known as Pimpa or Seetu, traditionally consumed as a vegetable. Our research aimed to comprehensively analyze its phytochemical constituents, and evaluate its antibacterial, antioxidant, antiviral, anti-inflammatory, antidiabetic, and antipyretic potentials. Utilizing a range of extracts including methanol (MtOH), ethanol (EtOH), ethyl acetate (EA), n-hexane (n-hex), dichloromethane (DCM), and aqueous (Aq), for effective extraction of phytochemicals from C. edulis. Standard biochemical assays and High-Performance Liquid Chromatography-Photodiode Array (HPLC-PDA) were used for analysis of phenolic compounds. Antibacterial effect(s) were confirmed through disc diffusion method and min inhibitory concentrations (MICs). The antioxidant activity was assessed through the Ferric Reducing Antioxidant Power (FRAP) assay and the DPPH radical scavenging method. In vivo antiviral potential was assessed through Hemagglutination (HA) test. Anti-inflammatory, antidiabetic, and antipyretic activities were performed on female albino rats using carrageenan, alloxan monohydrate and yeast-induced methods, respectively. Statistical analysis was done using standard one way ANOVA.Our findings revealed a rich diversity of phenolic compounds and the presence of proteins, alkaloids, and carbohydrates in C. edulis. MtOH and n-hex extracts demonstrated deep antiviral activity against various viral strains. In vivo toxicology studies indicated no significant toxicity at doses up to 5 g/kg. The DCM extract has shown notable anti-inflammatory effects, and EA extract was leading in antipyretic activity. All extracts, except MtOH, exhibited antidiabetic properties.In conclusion, C. edulis emerges not only as a valuable nutritional source but also as a potent alternative medicinal resource, offering wide range of therapeutic benefits.http://www.sciencedirect.com/science/article/pii/S1018364724004312C. edulisCholistanAntioxidantAntidiabeticAnti-corona virusAnti-inflammatory
spellingShingle Bushra Gillani
Sarah Tariq
Mirza Imran Shahzad
Tatheer Fatima
Marcello Locatelli
Xinxia Cai
Adnan Noor Shah
Ajaz Ahmad
Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant
Journal of King Saud University: Science
C. edulis
Cholistan
Antioxidant
Antidiabetic
Anti-corona virus
Anti-inflammatory
title Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant
title_full Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant
title_fullStr Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant
title_full_unstemmed Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant
title_short Phytochemical composition and therapeutic potential of Caralluma edulis a cholistani plant
title_sort phytochemical composition and therapeutic potential of caralluma edulis a cholistani plant
topic C. edulis
Cholistan
Antioxidant
Antidiabetic
Anti-corona virus
Anti-inflammatory
url http://www.sciencedirect.com/science/article/pii/S1018364724004312
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