DMSO-free cryopreservation of hiPSC-derived cardiomyocytes: low temperature characterization and protocol development

DMSO-free cryopreservation of hiPSC-derived cardiomyocytes: low temperature characterization and protocol development

Abstract Background Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have attracted significant interest for use in disease modeling, drug discovery and potential therapeutic applications. However, conventional hiPSC-CM cryopreservation protocols largely use dimethyl sulfoxide...

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Bibliographic Details
Main Authors: Akshat S. Mallya, Tessa Burrows, Jeanne Hsieh, Troy Louwagie, James R. Dutton, Brenda M. Ogle, Allison Hubel
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Stem Cell Research & Therapy
Subjects:
Cardiomyocytes
Cryopreservation
DMSO-free
Raman spectroscopy
NADES
Protocol development
Online Access:https://doi.org/10.1186/s13287-025-04384-5
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