A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY
<p><strong>Introduction</strong>: The MPAL comprise 2-5% of all acute leukemia. The present WHO 2008 classification has separated two groups in MPAL based on t(9;22) positivity and MLL rearrangement. <strong>Aims &amp; Objectives<...
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| Format: | Article |
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PAGEPress Publications
2012-01-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
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| Online Access: | http://www.mjhid.org/article/view/10111 |
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| author | PRATEEK bhatia JOGESHWAR BINOTA Neelam Varma DEEPAK BANSAL amita trehan RAM KUMAR MARWAHA PANKAJ MALHOTRA SUBHASH VARMA |
| author_facet | PRATEEK bhatia JOGESHWAR BINOTA Neelam Varma DEEPAK BANSAL amita trehan RAM KUMAR MARWAHA PANKAJ MALHOTRA SUBHASH VARMA |
| author_sort | PRATEEK bhatia |
| collection | DOAJ |
| description | <p><strong>Introduction</strong>: The MPAL comprise 2-5% of all acute leukemia. The present WHO 2008 classification has separated two groups in MPAL based on t(9;22) positivity and MLL rearrangement. <strong>Aims &amp; Objectives</strong>: The aim of the present pilot study is to note the incidence of BCR-ABL transcript in MPAL cases using the RT-PCR assay and to correlate the status with hematological remission post induction. <strong>Materials &amp; Methods</strong>: A total of 10 MPAL cases classified on Flow-cytometry based on the current WHO 2008 criteria were enrolled. In all the cases Bone marrow or peripheral blood sample in EDTA was processed for molecular studies and the RT-PCR reaction carried out using primers specific to the t (9;22) and t(4;11) translocation. The post induction check marrow slides were also reviewed. <strong>Results</strong>: Out of the total 10 MPAL cases, 7/10 (70%) were adult and 3/10 (30%) pediatric cases. A total of 4/10 (40%) cases showed positivity for the t(9;22) transcript and none for t (4;11). Of the 4 positive cases, 3/10(30%) were adult cases and 1/10(10%) pediatric case. The BCR-ABL transcript type in adult cases was b3a2 (p210) in 2/3 (66%) and e1a2 (p190) in 1/3 (33.3%) case. The single pediatric case was positive for b3a2 transcript. <strong>Discussion &amp; Conclusion</strong>: All the 4 positive MPAL cases presented with high TLC and low platelet count (p&lt;0.05). The positive cases also showed hematological remission at post induction check marrow (blasts&lt;5%). This could partly be explained due to good response to the imatinib added to the treatment protocol.</p> |
| format | Article |
| id | doaj-art-e548d2a926af42f5a4e886fbfb1fc0f3 |
| institution | Kabale University |
| issn | 2035-3006 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj-art-e548d2a926af42f5a4e886fbfb1fc0f32024-12-02T00:02:54ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062012-01-0141e2012024e2012024A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPYPRATEEK bhatiaJOGESHWAR BINOTANeelam VarmaDEEPAK BANSALamita trehanRAM KUMAR MARWAHAPANKAJ MALHOTRASUBHASH VARMA<p><strong>Introduction</strong>: The MPAL comprise 2-5% of all acute leukemia. The present WHO 2008 classification has separated two groups in MPAL based on t(9;22) positivity and MLL rearrangement. <strong>Aims &amp; Objectives</strong>: The aim of the present pilot study is to note the incidence of BCR-ABL transcript in MPAL cases using the RT-PCR assay and to correlate the status with hematological remission post induction. <strong>Materials &amp; Methods</strong>: A total of 10 MPAL cases classified on Flow-cytometry based on the current WHO 2008 criteria were enrolled. In all the cases Bone marrow or peripheral blood sample in EDTA was processed for molecular studies and the RT-PCR reaction carried out using primers specific to the t (9;22) and t(4;11) translocation. The post induction check marrow slides were also reviewed. <strong>Results</strong>: Out of the total 10 MPAL cases, 7/10 (70%) were adult and 3/10 (30%) pediatric cases. A total of 4/10 (40%) cases showed positivity for the t(9;22) transcript and none for t (4;11). Of the 4 positive cases, 3/10(30%) were adult cases and 1/10(10%) pediatric case. The BCR-ABL transcript type in adult cases was b3a2 (p210) in 2/3 (66%) and e1a2 (p190) in 1/3 (33.3%) case. The single pediatric case was positive for b3a2 transcript. <strong>Discussion &amp; Conclusion</strong>: All the 4 positive MPAL cases presented with high TLC and low platelet count (p&lt;0.05). The positive cases also showed hematological remission at post induction check marrow (blasts&lt;5%). This could partly be explained due to good response to the imatinib added to the treatment protocol.</p>http://www.mjhid.org/article/view/10111Adult, Mixed Phenotype Acute Leukemia, Pediatric, Reverse Transcriptase-PCR, Transcript |
| spellingShingle | PRATEEK bhatia JOGESHWAR BINOTA Neelam Varma DEEPAK BANSAL amita trehan RAM KUMAR MARWAHA PANKAJ MALHOTRA SUBHASH VARMA A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY Mediterranean Journal of Hematology and Infectious Diseases Adult, Mixed Phenotype Acute Leukemia, Pediatric, Reverse Transcriptase-PCR, Transcript |
| title | A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY |
| title_full | A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY |
| title_fullStr | A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY |
| title_full_unstemmed | A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY |
| title_short | A STUDY ON THE EXPRESSION OF BCR-ABL TRANSCRIPT IN MIXED PHENOTYPE ACUTE LEUKEMIA (MPAL) CASES USING THE REVERSE TRANSCRIPTASE POLYMERASE REACTION ASSAY (RT-PCR) AND ITS CORRELATION WITH HEMATOLOGICAL REMISSION STATUS POST INITIAL INDUCTION THERAPY |
| title_sort | study on the expression of bcr abl transcript in mixed phenotype acute leukemia mpal cases using the reverse transcriptase polymerase reaction assay rt pcr and its correlation with hematological remission status post initial induction therapy |
| topic | Adult, Mixed Phenotype Acute Leukemia, Pediatric, Reverse Transcriptase-PCR, Transcript |
| url | http://www.mjhid.org/article/view/10111 |
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