IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections
Introduction: The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of Plasmodium vivax-merozoite surface protein-1 (PvMSP1) to detect parasite clones. Methods: We applied amplicon de...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Global Infectious Diseases |
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| Online Access: | https://journals.lww.com/10.4103/jgid.jgid_35_24 |
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| author | Fernanda de Almeida Batalha Elizangela Farias da Silva Paula Taquita Serra Rafaella Oliveira dos Santos Zeca Manuel Salimo Yury de Oliveira Chaves Mirian Ívens Fagundes Paulo Emílio Feuser Victor Costa de Souza Felipe Gomes Naveca Ricardo A. Machado de Ávila Paulo Afonso Nogueira |
| author_facet | Fernanda de Almeida Batalha Elizangela Farias da Silva Paula Taquita Serra Rafaella Oliveira dos Santos Zeca Manuel Salimo Yury de Oliveira Chaves Mirian Ívens Fagundes Paulo Emílio Feuser Victor Costa de Souza Felipe Gomes Naveca Ricardo A. Machado de Ávila Paulo Afonso Nogueira |
| author_sort | Fernanda de Almeida Batalha |
| collection | DOAJ |
| description | Introduction:
The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of Plasmodium vivax-merozoite surface protein-1 (PvMSP1) to detect parasite clones.
Methods:
We applied amplicon deep sequencing (ADS) of block 2 region of the MSP-1 gene (pvmsp1) to determine cocirculating parasite clones within eight P. vivax-infected individuals. Based on this, a seroprevalence of IgM and IgG antibodies against sets of peptides of different block-2 haplotypes was validated. After, we evaluated the seroprevalence in plasma of 72 pregnant women, from which 31 had recurrent P. vivax infections.
Results:
ADS revealed one block 2 haplotype clone infecting five of eight P. vivax-infected individuals. In all, IgM antibodies, not IgG, recognized only a set of peptides specific to the block 2 haplotype determined by ADS. In the other three patients, ADS determined three concurrent block 2 haplotype clones, among whom there was always one haplotype that predominated with more than 95% of high-quality reads and two other smaller haplotypes with up to 5% and the least was <1%. We observed higher IgM levels against haplotype-specific peptides corresponding to the predominant clone. The seroprevalence of pregnant women showed that anti-haplotype-specific IgM detected coinfection with parasite clones per pregnant woman and we also observed levels of anti-haplotype-specific IgM in primary infection increased in some recurrent episodes.
Conclusion:
IgM against sets of peptides specific to different pvmsp1 haplotypes may be employed as a serological marker for parasite clones in vivax malaria. |
| format | Article |
| id | doaj-art-e52e82c4d57d49dd952f111a80be609e |
| institution | Kabale University |
| issn | 0974-777X 0974-8245 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Global Infectious Diseases |
| spelling | doaj-art-e52e82c4d57d49dd952f111a80be609e2025-01-07T07:10:17ZengWolters Kluwer Medknow PublicationsJournal of Global Infectious Diseases0974-777X0974-82452024-12-0116415215910.4103/jgid.jgid_35_24IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax InfectionsFernanda de Almeida BatalhaElizangela Farias da SilvaPaula Taquita SerraRafaella Oliveira dos SantosZeca Manuel SalimoYury de Oliveira ChavesMirian Ívens FagundesPaulo Emílio FeuserVictor Costa de SouzaFelipe Gomes NavecaRicardo A. Machado de ÁvilaPaulo Afonso NogueiraIntroduction: The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of Plasmodium vivax-merozoite surface protein-1 (PvMSP1) to detect parasite clones. Methods: We applied amplicon deep sequencing (ADS) of block 2 region of the MSP-1 gene (pvmsp1) to determine cocirculating parasite clones within eight P. vivax-infected individuals. Based on this, a seroprevalence of IgM and IgG antibodies against sets of peptides of different block-2 haplotypes was validated. After, we evaluated the seroprevalence in plasma of 72 pregnant women, from which 31 had recurrent P. vivax infections. Results: ADS revealed one block 2 haplotype clone infecting five of eight P. vivax-infected individuals. In all, IgM antibodies, not IgG, recognized only a set of peptides specific to the block 2 haplotype determined by ADS. In the other three patients, ADS determined three concurrent block 2 haplotype clones, among whom there was always one haplotype that predominated with more than 95% of high-quality reads and two other smaller haplotypes with up to 5% and the least was <1%. We observed higher IgM levels against haplotype-specific peptides corresponding to the predominant clone. The seroprevalence of pregnant women showed that anti-haplotype-specific IgM detected coinfection with parasite clones per pregnant woman and we also observed levels of anti-haplotype-specific IgM in primary infection increased in some recurrent episodes. Conclusion: IgM against sets of peptides specific to different pvmsp1 haplotypes may be employed as a serological marker for parasite clones in vivax malaria.https://journals.lww.com/10.4103/jgid.jgid_35_24anti-block 2 merozoite surface protein 1igmplasmodium vivaxpregnant womanrecurrence |
| spellingShingle | Fernanda de Almeida Batalha Elizangela Farias da Silva Paula Taquita Serra Rafaella Oliveira dos Santos Zeca Manuel Salimo Yury de Oliveira Chaves Mirian Ívens Fagundes Paulo Emílio Feuser Victor Costa de Souza Felipe Gomes Naveca Ricardo A. Machado de Ávila Paulo Afonso Nogueira IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections Journal of Global Infectious Diseases anti-block 2 merozoite surface protein 1 igm plasmodium vivax pregnant woman recurrence |
| title | IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections |
| title_full | IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections |
| title_fullStr | IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections |
| title_full_unstemmed | IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections |
| title_short | IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Plasmodium vivax Infections |
| title_sort | igm against merozoite surface protein 1 block 2 haplotypes as new tools for plasmodium vivax infections |
| topic | anti-block 2 merozoite surface protein 1 igm plasmodium vivax pregnant woman recurrence |
| url | https://journals.lww.com/10.4103/jgid.jgid_35_24 |
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