Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients
Abstract Objective The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxa...
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2024-12-01
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| Online Access: | https://doi.org/10.1186/s13104-024-07047-5 |
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| author | Kosar Salmani Nasrabadi Yasaman Ahmadi Seyed Mahdi Ghasemi Dariush Shokri |
| author_facet | Kosar Salmani Nasrabadi Yasaman Ahmadi Seyed Mahdi Ghasemi Dariush Shokri |
| author_sort | Kosar Salmani Nasrabadi |
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| description | Abstract Objective The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii. Results In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla OXA−51, bla OXA−23, blaOXA−58, bla NDM, bla IMP, and bla VIM genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla OXA−23 gene, 23 (92.0%) contained the bla OXA−51 gene, and 1 (4.0%) isolate carried the bla NDM gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla OXA−51 and bla OXA−23 genes (oxacillinase genes) were dominant genes, but bla IMP and bla OXA−58 were not local carbapenem resistant genes in Isfahan. |
| format | Article |
| id | doaj-art-e520d304f5304a868da9b1aaf8042e4e |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-e520d304f5304a868da9b1aaf8042e4e2025-01-05T12:07:36ZengBMCBMC Research Notes1756-05002024-12-011711610.1186/s13104-024-07047-5Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patientsKosar Salmani Nasrabadi0Yasaman Ahmadi1Seyed Mahdi Ghasemi2Dariush Shokri3Department of Microbiology, Faculty of Biological Sciences and Technology Shahid Ashrafi, Shahid Ashraf Esfahani UniversityDepartment of Microbiology, Kish International Branch of Islamic Azad UniversityDepartment of Microbiology, Faculty of Biological Sciences and Technology Shahid Ashrafi, Shahid Ashraf Esfahani UniversityNosocomial Infection Research Center, Isfahan University of Medical SciencesAbstract Objective The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii. Results In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla OXA−51, bla OXA−23, blaOXA−58, bla NDM, bla IMP, and bla VIM genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla OXA−23 gene, 23 (92.0%) contained the bla OXA−51 gene, and 1 (4.0%) isolate carried the bla NDM gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla OXA−51 and bla OXA−23 genes (oxacillinase genes) were dominant genes, but bla IMP and bla OXA−58 were not local carbapenem resistant genes in Isfahan.https://doi.org/10.1186/s13104-024-07047-5Acinetobacter baumanniiCarbapenem-resistantMultidrug-resistant strains |
| spellingShingle | Kosar Salmani Nasrabadi Yasaman Ahmadi Seyed Mahdi Ghasemi Dariush Shokri Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients BMC Research Notes Acinetobacter baumannii Carbapenem-resistant Multidrug-resistant strains |
| title | Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients |
| title_full | Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients |
| title_fullStr | Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients |
| title_full_unstemmed | Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients |
| title_short | Identification of bla OXA−51−23−58, bla VIM , bla NDM, and bla IMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients |
| title_sort | identification of bla oxa 51 23 58 bla vim bla ndm and bla imp carbapenemase genes in acinetobacter baumannii isolates from hospitalized patients |
| topic | Acinetobacter baumannii Carbapenem-resistant Multidrug-resistant strains |
| url | https://doi.org/10.1186/s13104-024-07047-5 |
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