Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis
<b>Aim:</b> Mastitis is one of the most common diseases of dairy cattle and causes significant economic losses. Staphylococcus aureus produces many virulence factors that facilitate the adhesion and penetration of damaged tissues, and thereby, cause subclinical mastitis. This study was a...
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| Language: | English |
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Selcuk University Press
2020-06-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1289 |
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| author | Mustafa Mohammed Saeed Al-Rubaye Hasan Hüseyin Hadimli |
| author_facet | Mustafa Mohammed Saeed Al-Rubaye Hasan Hüseyin Hadimli |
| author_sort | Mustafa Mohammed Saeed Al-Rubaye |
| collection | DOAJ |
| description | <b>Aim:</b> Mastitis is one of the most common diseases of dairy cattle and
causes significant economic losses. Staphylococcus aureus produces
many virulence factors that facilitate the adhesion and penetration
of damaged tissues, and thereby, cause subclinical mastitis. This
study was aimed at investigating the phenotypic and genotypic characteristics
and antimicrobial susceptibility of S. aureus..<p>
<b>Materials and Methods: </b>A total of 241 S. aureus strains isolated
from bovine mastitis cases were tested phenotypically (catalase,
coagulase, haemolysis, DNase, mannitol fermentation and biofilm
formation) and genotypically (by the polymerase chain reaction
(PCR) technique). Antimicrobial susceptibility was tested using 15
different antibiotics.<p>
<b>Results:</b> While the isolates showed different levels of haemolytic activity
(? 47%, ? 42%, ? 10% and ? 1%), only the ?-haemolytic strains
produced a positive CAMP-like reaction. Although all isolates were
able to grow on MSA containing 7.5% NaCl, mannitol fermentation
activity was observed in 80.5% of the isolates. The nuc gene was detected
in all isolates, but only 84.2% of the isolates showed DNase
activity. The Congo red agar method can be used to detect the biofilm
forming capability of isolates, but the crystal violet staining method
gives more reliable results. The sec gene was the most common enterotoxin
genes (84%). Three isolates harboured the mecA gene, but
were sensitive to methicillin.<p>
<b>Conclusion:</b> Phenotypic variations among isolates result in the
misclassification of S. aureus strains and require the use of molecular
methods. The rapid and accurate molecular typing of S. aureus can
aid in both determining the prevalence of this infectious microorganism
and preventing epidemic infections. |
| format | Article |
| id | doaj-art-e5143ff26ffd4de78bd3b2783dce0e1b |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-e5143ff26ffd4de78bd3b2783dce0e1b2024-12-02T09:05:28ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532020-06-013621271391289Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitisMustafa Mohammed Saeed Al-RubayeHasan Hüseyin Hadimli<b>Aim:</b> Mastitis is one of the most common diseases of dairy cattle and causes significant economic losses. Staphylococcus aureus produces many virulence factors that facilitate the adhesion and penetration of damaged tissues, and thereby, cause subclinical mastitis. This study was aimed at investigating the phenotypic and genotypic characteristics and antimicrobial susceptibility of S. aureus..<p> <b>Materials and Methods: </b>A total of 241 S. aureus strains isolated from bovine mastitis cases were tested phenotypically (catalase, coagulase, haemolysis, DNase, mannitol fermentation and biofilm formation) and genotypically (by the polymerase chain reaction (PCR) technique). Antimicrobial susceptibility was tested using 15 different antibiotics.<p> <b>Results:</b> While the isolates showed different levels of haemolytic activity (? 47%, ? 42%, ? 10% and ? 1%), only the ?-haemolytic strains produced a positive CAMP-like reaction. Although all isolates were able to grow on MSA containing 7.5% NaCl, mannitol fermentation activity was observed in 80.5% of the isolates. The nuc gene was detected in all isolates, but only 84.2% of the isolates showed DNase activity. The Congo red agar method can be used to detect the biofilm forming capability of isolates, but the crystal violet staining method gives more reliable results. The sec gene was the most common enterotoxin genes (84%). Three isolates harboured the mecA gene, but were sensitive to methicillin.<p> <b>Conclusion:</b> Phenotypic variations among isolates result in the misclassification of S. aureus strains and require the use of molecular methods. The rapid and accurate molecular typing of S. aureus can aid in both determining the prevalence of this infectious microorganism and preventing epidemic infections.http://eurasianjvetsci.org/pdf.php3?id=1289staphylococcus aureusphenotypinggenotypingantimicrobial susceptibility |
| spellingShingle | Mustafa Mohammed Saeed Al-Rubaye Hasan Hüseyin Hadimli Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis Eurasian Journal of Veterinary Sciences staphylococcus aureus phenotyping genotyping antimicrobial susceptibility |
| title | Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis |
| title_full | Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis |
| title_fullStr | Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis |
| title_full_unstemmed | Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis |
| title_short | Determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis |
| title_sort | determination of phenotypical and genotypical characterization and antimicrobial resistance genes of staphylococcus aureus isolated from milk of dairy cows with mastitis |
| topic | staphylococcus aureus phenotyping genotyping antimicrobial susceptibility |
| url | http://eurasianjvetsci.org/pdf.php3?id=1289 |
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