Mechanism and Kinetics of HIV-1 Protease Activation
The HIV-1 protease is a critical enzyme for viral replication. Because protease activity is necessary to generate mature infectious virions, it is a primary target of antiretroviral treatment. Here, we provide an overview of the mechanisms regulating protease activation and the methods available to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/12/1826 |
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| author | Caroline O. Tabler John C. Tilton |
| author_facet | Caroline O. Tabler John C. Tilton |
| author_sort | Caroline O. Tabler |
| collection | DOAJ |
| description | The HIV-1 protease is a critical enzyme for viral replication. Because protease activity is necessary to generate mature infectious virions, it is a primary target of antiretroviral treatment. Here, we provide an overview of the mechanisms regulating protease activation and the methods available to assess protease activity. Finally, we will highlight some of the key discoveries regarding the kinetics of protease activation from the last decade, including how the manipulation of activation kinetics may provide novel HIV-1 treatment strategies. |
| format | Article |
| id | doaj-art-e50fb9dfb6e94ab5ae522c90b16b76c6 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-e50fb9dfb6e94ab5ae522c90b16b76c62024-12-27T14:58:57ZengMDPI AGViruses1999-49152024-11-011612182610.3390/v16121826Mechanism and Kinetics of HIV-1 Protease ActivationCaroline O. Tabler0John C. Tilton1Center for Proteomics and Bioinformatics, Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USACenter for Proteomics and Bioinformatics, Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USAThe HIV-1 protease is a critical enzyme for viral replication. Because protease activity is necessary to generate mature infectious virions, it is a primary target of antiretroviral treatment. Here, we provide an overview of the mechanisms regulating protease activation and the methods available to assess protease activity. Finally, we will highlight some of the key discoveries regarding the kinetics of protease activation from the last decade, including how the manipulation of activation kinetics may provide novel HIV-1 treatment strategies.https://www.mdpi.com/1999-4915/16/12/1826HIV-1proteasematurationribosomal frameshiftESCRTnanoscale flow cytometry |
| spellingShingle | Caroline O. Tabler John C. Tilton Mechanism and Kinetics of HIV-1 Protease Activation Viruses HIV-1 protease maturation ribosomal frameshift ESCRT nanoscale flow cytometry |
| title | Mechanism and Kinetics of HIV-1 Protease Activation |
| title_full | Mechanism and Kinetics of HIV-1 Protease Activation |
| title_fullStr | Mechanism and Kinetics of HIV-1 Protease Activation |
| title_full_unstemmed | Mechanism and Kinetics of HIV-1 Protease Activation |
| title_short | Mechanism and Kinetics of HIV-1 Protease Activation |
| title_sort | mechanism and kinetics of hiv 1 protease activation |
| topic | HIV-1 protease maturation ribosomal frameshift ESCRT nanoscale flow cytometry |
| url | https://www.mdpi.com/1999-4915/16/12/1826 |
| work_keys_str_mv | AT carolineotabler mechanismandkineticsofhiv1proteaseactivation AT johnctilton mechanismandkineticsofhiv1proteaseactivation |