Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS
The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years (the first, second and third year sample) were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography (GC-TOF-MS) to study the effects of...
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250182 |
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| author | Cong Li Yingling Zou Guozhou Liao Zijiang Yang Dahai Gu Yuehong Pu Changrong Ge Guiying Wang |
| author_facet | Cong Li Yingling Zou Guozhou Liao Zijiang Yang Dahai Gu Yuehong Pu Changrong Ge Guiying Wang |
| author_sort | Cong Li |
| collection | DOAJ |
| description | The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years (the first, second and third year sample) were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography (GC-TOF-MS) to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing years. The results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes, the dominant fungi phyla were Ascomycota and Basidiomycota, while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham, respectively. Totally, 252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years, and 12 different metabolites were screened through multivariate statistical analysis. Further metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation, of which 8 metabolic pathways had significant effects on ham fermentation (Impact > 0.01, P < 0.05). The content of L-proline, phenyllactic acid, L-lysine, carnosine, taurine, D-proline, betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia, but negatively correlated with the relative abundance of Halomonas, Aspergillus and Yamadazyma. |
| format | Article |
| id | doaj-art-e50fb629827a4fb4869b7f3df02e991f |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-e50fb629827a4fb4869b7f3df02e991f2025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342187219610.26599/FSHW.2022.9250182Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MSCong Li0Yingling Zou1Guozhou Liao2Zijiang Yang3Dahai Gu4Yuehong Pu5Changrong Ge6Guiying Wang7Livestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaLivestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, ChinaThe internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years (the first, second and third year sample) were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography (GC-TOF-MS) to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing years. The results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes, the dominant fungi phyla were Ascomycota and Basidiomycota, while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham, respectively. Totally, 252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years, and 12 different metabolites were screened through multivariate statistical analysis. Further metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation, of which 8 metabolic pathways had significant effects on ham fermentation (Impact > 0.01, P < 0.05). The content of L-proline, phenyllactic acid, L-lysine, carnosine, taurine, D-proline, betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia, but negatively correlated with the relative abundance of Halomonas, Aspergillus and Yamadazyma.https://www.sciopen.com/article/10.26599/FSHW.2022.9250182nuodeng hammicrobial diversitysmall molecule metaboliteshigh-throughput sequencinggas chromatography-time of flight mass spectrography |
| spellingShingle | Cong Li Yingling Zou Guozhou Liao Zijiang Yang Dahai Gu Yuehong Pu Changrong Ge Guiying Wang Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS Food Science and Human Wellness nuodeng ham microbial diversity small molecule metabolites high-throughput sequencing gas chromatography-time of flight mass spectrography |
| title | Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS |
| title_full | Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS |
| title_fullStr | Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS |
| title_full_unstemmed | Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS |
| title_short | Variation of microbiological and small molecule metabolite profiles of Nuodeng ham during ripening by high-throughput sequencing and GC-TOF-MS |
| title_sort | variation of microbiological and small molecule metabolite profiles of nuodeng ham during ripening by high throughput sequencing and gc tof ms |
| topic | nuodeng ham microbial diversity small molecule metabolites high-throughput sequencing gas chromatography-time of flight mass spectrography |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250182 |
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