Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications
ABSTRACT Extracellular vesicles (EVs) play a key role in cell‐to‐cell communication and have drawn significant attention due to their potential clinical applications. However, much remains to be understood about the biology of EV‐associated DNA (EV‐DNA). EV‐DNA is actively released by both normal an...
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| Format: | Article |
| Language: | English |
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Wiley
2025-03-01
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| Series: | Journal of Extracellular Vesicles |
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| Online Access: | https://doi.org/10.1002/jev2.70047 |
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| author | Jamal Ghanam Kristína Lichá Venkatesh Kumar Chetty Ommolbanin Asad Pour Dirk Reinhardt Barbora Tamášová Peter Hoyer Jan Lötvall Basant Kumar Thakur |
| author_facet | Jamal Ghanam Kristína Lichá Venkatesh Kumar Chetty Ommolbanin Asad Pour Dirk Reinhardt Barbora Tamášová Peter Hoyer Jan Lötvall Basant Kumar Thakur |
| author_sort | Jamal Ghanam |
| collection | DOAJ |
| description | ABSTRACT Extracellular vesicles (EVs) play a key role in cell‐to‐cell communication and have drawn significant attention due to their potential clinical applications. However, much remains to be understood about the biology of EV‐associated DNA (EV‐DNA). EV‐DNA is actively released by both normal and malignant cells and consists of diverse fragments with varying structures. Because EV‐DNA spans the entire genome of cells from which it originates, it continues to be attractive as a biomarker for cancer diagnosis and monitoring. Further, EV‐DNA delivery can alter the function of recipient cells by interfering with cytoplasmic DNA sensor pathways. This review explores the biology and significance of EV‐DNA, including its topology and fragmentomics features, modality of association with EVs, packaging mechanisms, and potential functions. It also emphasizes the specificity of vesicular DNA in identifying genetic and epigenetic changes in cancer. Additionally, it delves into the impact of EV‐DNA on cellular behaviour and its potential use as a therapeutic target in cancer. The review discusses new insights into EV‐DNA biology and provides perspectives and alternatives to address the challenges and concerns for future EV‐DNA studies. |
| format | Article |
| id | doaj-art-e4e7ddb6b3e8432d978fbc59c25e9de7 |
| institution | Kabale University |
| issn | 2001-3078 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj-art-e4e7ddb6b3e8432d978fbc59c25e9de72025-08-20T03:49:07ZengWileyJournal of Extracellular Vesicles2001-30782025-03-01143n/an/a10.1002/jev2.70047Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical ApplicationsJamal Ghanam0Kristína Lichá1Venkatesh Kumar Chetty2Ommolbanin Asad Pour3Dirk Reinhardt4Barbora Tamášová5Peter Hoyer6Jan Lötvall7Basant Kumar Thakur8Department of Pediatrics IIIUniversity Hospital EssenEssenGermanyDepartment of Pediatrics IIIUniversity Hospital EssenEssenGermanyDepartment of Pediatrics IIIUniversity Hospital EssenEssenGermanyDepartment of Pediatrics IIIUniversity Hospital EssenEssenGermanyAML‐BFM Study GroupInstitute of Molecular Biomedicine, Faculty of MedicineComenius UniversityBratislavaSlovakiaDepartment of Pediatrics IIUniversity Hospital Essen, University of Duisburg‐EssenEssenGermanyKrefting Research Centre, Institute of Medicine, Sahlgrenska AcademyUniversity of GothenburgGothenburgSwedenDepartment of Pediatrics IIIUniversity Hospital EssenEssenGermanyABSTRACT Extracellular vesicles (EVs) play a key role in cell‐to‐cell communication and have drawn significant attention due to their potential clinical applications. However, much remains to be understood about the biology of EV‐associated DNA (EV‐DNA). EV‐DNA is actively released by both normal and malignant cells and consists of diverse fragments with varying structures. Because EV‐DNA spans the entire genome of cells from which it originates, it continues to be attractive as a biomarker for cancer diagnosis and monitoring. Further, EV‐DNA delivery can alter the function of recipient cells by interfering with cytoplasmic DNA sensor pathways. This review explores the biology and significance of EV‐DNA, including its topology and fragmentomics features, modality of association with EVs, packaging mechanisms, and potential functions. It also emphasizes the specificity of vesicular DNA in identifying genetic and epigenetic changes in cancer. Additionally, it delves into the impact of EV‐DNA on cellular behaviour and its potential use as a therapeutic target in cancer. The review discusses new insights into EV‐DNA biology and provides perspectives and alternatives to address the challenges and concerns for future EV‐DNA studies.https://doi.org/10.1002/jev2.70047biomarkerEV‐DNAEV‐DNA packagingEV‐DNA topologyextracellular vesicles |
| spellingShingle | Jamal Ghanam Kristína Lichá Venkatesh Kumar Chetty Ommolbanin Asad Pour Dirk Reinhardt Barbora Tamášová Peter Hoyer Jan Lötvall Basant Kumar Thakur Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications Journal of Extracellular Vesicles biomarker EV‐DNA EV‐DNA packaging EV‐DNA topology extracellular vesicles |
| title | Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications |
| title_full | Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications |
| title_fullStr | Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications |
| title_full_unstemmed | Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications |
| title_short | Unravelling the Significance of Extracellular Vesicle‐Associated DNA in Cancer Biology and Its Potential Clinical Applications |
| title_sort | unravelling the significance of extracellular vesicle associated dna in cancer biology and its potential clinical applications |
| topic | biomarker EV‐DNA EV‐DNA packaging EV‐DNA topology extracellular vesicles |
| url | https://doi.org/10.1002/jev2.70047 |
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