Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements
Background Procalcitonin (PCT) is a crucial biomarker for diagnosing sepsis and managing antibiotic therapy. This study evaluated the analytical performance and comparability of the Access PCT and Elecsys BRAHMS PCT assays. Methods The precision, detection capability, linearity, and reference range...
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Kosin University College of Medicine
2024-12-01
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| Series: | Kosin Medical Journal |
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| Online Access: | http://www.kosinmedj.org/upload/pdf/kmj-24-155.pdf |
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| author | Hyunji Choi Sang-Shin Lee Hyunyong Hwang |
| author_facet | Hyunji Choi Sang-Shin Lee Hyunyong Hwang |
| author_sort | Hyunji Choi |
| collection | DOAJ |
| description | Background Procalcitonin (PCT) is a crucial biomarker for diagnosing sepsis and managing antibiotic therapy. This study evaluated the analytical performance and comparability of the Access PCT and Elecsys BRAHMS PCT assays. Methods The precision, detection capability, linearity, and reference range of both assays were assessed. A comparative analysis included 182 patient samples categorized into four risk groups to compare the results between Access PCT and Elecsys BRAHMS PCT assays. Results The Access PCT assay demonstrated precision within the manufacturer’s threshold, and its detection capabilities were verified. This assay exhibited excellent linearity and appropriate reference intervals. Comparative analysis indicated that the Access PCT assay reported higher overall PCT levels than the Elecsys BRAHMS assay, with high agreement between the assays (κ=0.941). However, the biases varied across different PCT concentration intervals. Conclusions Both the Access PCT and Elecsys BRAHMS PCT assays performed robustly with notable concordance but varying biases at different concentration intervals. The observed biases require careful consideration in clinical decision-making, especially when adopting novel assay systems. Standardizing the calibration across different platforms is recommended to improve assay comparability. |
| format | Article |
| id | doaj-art-e49eeab7afdc4708801b4a79f08d76ef |
| institution | Kabale University |
| issn | 2005-9531 2586-7024 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Kosin University College of Medicine |
| record_format | Article |
| series | Kosin Medical Journal |
| spelling | doaj-art-e49eeab7afdc4708801b4a79f08d76ef2024-12-30T04:43:15ZengKosin University College of MedicineKosin Medical Journal2005-95312586-70242024-12-0139427228010.7180/kmj.24.1551308Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurementsHyunji Choi0Sang-Shin Lee1Hyunyong Hwang2 Department of Laboratory Medicine, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, Korea Department of Psychiatry, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, Korea Department of Laboratory Medicine, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, KoreaBackground Procalcitonin (PCT) is a crucial biomarker for diagnosing sepsis and managing antibiotic therapy. This study evaluated the analytical performance and comparability of the Access PCT and Elecsys BRAHMS PCT assays. Methods The precision, detection capability, linearity, and reference range of both assays were assessed. A comparative analysis included 182 patient samples categorized into four risk groups to compare the results between Access PCT and Elecsys BRAHMS PCT assays. Results The Access PCT assay demonstrated precision within the manufacturer’s threshold, and its detection capabilities were verified. This assay exhibited excellent linearity and appropriate reference intervals. Comparative analysis indicated that the Access PCT assay reported higher overall PCT levels than the Elecsys BRAHMS assay, with high agreement between the assays (κ=0.941). However, the biases varied across different PCT concentration intervals. Conclusions Both the Access PCT and Elecsys BRAHMS PCT assays performed robustly with notable concordance but varying biases at different concentration intervals. The observed biases require careful consideration in clinical decision-making, especially when adopting novel assay systems. Standardizing the calibration across different platforms is recommended to improve assay comparability.http://www.kosinmedj.org/upload/pdf/kmj-24-155.pdfagreementlimit of detectionlinearityprecisionprocalcitonin |
| spellingShingle | Hyunji Choi Sang-Shin Lee Hyunyong Hwang Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements Kosin Medical Journal agreement limit of detection linearity precision procalcitonin |
| title | Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements |
| title_full | Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements |
| title_fullStr | Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements |
| title_full_unstemmed | Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements |
| title_short | Comparative analysis of Access PCT and Elecsys BRAHMS PCT assays for procalcitonin measurements |
| title_sort | comparative analysis of access pct and elecsys brahms pct assays for procalcitonin measurements |
| topic | agreement limit of detection linearity precision procalcitonin |
| url | http://www.kosinmedj.org/upload/pdf/kmj-24-155.pdf |
| work_keys_str_mv | AT hyunjichoi comparativeanalysisofaccesspctandelecsysbrahmspctassaysforprocalcitoninmeasurements AT sangshinlee comparativeanalysisofaccesspctandelecsysbrahmspctassaysforprocalcitoninmeasurements AT hyunyonghwang comparativeanalysisofaccesspctandelecsysbrahmspctassaysforprocalcitoninmeasurements |