Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities
IntroductionIn the context of Litopenaeus vannamei aquaculture, the incorporation of oyster shells has proven beneficial for enhancing water quality and the growth conditions of the shrimp. Nonetheless, the specific effects of in-situ water treatment using oyster shells on water quality and shrimp g...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2024.1495938/full |
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| author | Xianke Huang Xianke Huang Xianke Huang Guoqiang Xiao Guoqiang Xiao Guoqiang Xiao Xiang Zhang Xiang Zhang Xiang Zhang Shuangshuang Teng Shuangshuang Teng Shuangshuang Teng Min Li Min Li Min Li Yilong Cai Yilong Cai Yilong Cai Ran Chen Ran Chen Ran Chen Xiaolin Huang Xiaolin Huang Xiaolin Huang |
| author_facet | Xianke Huang Xianke Huang Xianke Huang Guoqiang Xiao Guoqiang Xiao Guoqiang Xiao Xiang Zhang Xiang Zhang Xiang Zhang Shuangshuang Teng Shuangshuang Teng Shuangshuang Teng Min Li Min Li Min Li Yilong Cai Yilong Cai Yilong Cai Ran Chen Ran Chen Ran Chen Xiaolin Huang Xiaolin Huang Xiaolin Huang |
| author_sort | Xianke Huang |
| collection | DOAJ |
| description | IntroductionIn the context of Litopenaeus vannamei aquaculture, the incorporation of oyster shells has proven beneficial for enhancing water quality and the growth conditions of the shrimp. Nonetheless, the specific effects of in-situ water treatment using oyster shells on water quality and shrimp growth, along with the composition and succession dynamics of the microbial community within oyster shell biofilms, have yet to be thoroughly investigated.MethodsThis study established control, low-concentration, and high-concentration oyster shell addition groups to emulate the in-situ water treatment environment with oyster shells, with the objective of elucidating the impacts of oyster shell addition on the aquaculture setting.ResultsThe results showed that the addition of oyster shells could significantly improve the length (F = 12.248, P = 0.005), weight(F = 138.234, P < 0.001), and survival rate (F = 15.248, P < 0.001) of shrimp, while there were no significant differences in the length (F = -1.233, P = 0.267) and survival rate (F = -2.143, P = 0.076) between the high and low concentration groups. Additionally, oyster shell addition resulted in elevated phosphate levels (F = 74.92, P < 0.001 in Day 70), diminished nitrite levels (F = 5.276, P = 0.031 in Day 56), and increased nitrate concentrations (F = 9.421, P = 0.006 in Day 70). Within the biofilms, the relative abundances of Ruegeria, Tenacibaculum, BD2- 11_terrestrial_group, and Kapabacteriales exhibited significant declines over time, whereas the relative abundance of Nitrospira demonstrated a marked increase, ultimately emerging as the predominant bacterium (Relative abundance 31.8%) in the biofilms during the latter stages of the experiment. Nitrospira also exhibited a notably higher relative abundance in the microbial community of the experimental water group relative to the control group (F = 2.265, P = 0.001).DiscussionThe biofilm provided conditions for the proliferation of Nitrospira, thereby accelerating the transformation of nitrite into nitrate in the aquaculture system, which subsequently improved the shrimp farming conditions. This research offers valuable insights for the application of oyster shells in shrimp farming and contributes to the theoretical underpinnings necessary for advancing our understanding of the mechanisms through which oyster shell biofilms enhance water quality and foster shrimp health. |
| format | Article |
| id | doaj-art-e4889fcccff74e73a867b25aeb46521f |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj-art-e4889fcccff74e73a867b25aeb46521f2024-11-20T04:50:17ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452024-11-011110.3389/fmars.2024.14959381495938Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communitiesXianke Huang0Xianke Huang1Xianke Huang2Guoqiang Xiao3Guoqiang Xiao4Guoqiang Xiao5Xiang Zhang6Xiang Zhang7Xiang Zhang8Shuangshuang Teng9Shuangshuang Teng10Shuangshuang Teng11Min Li12Min Li13Min Li14Yilong Cai15Yilong Cai16Yilong Cai17Ran Chen18Ran Chen19Ran Chen20Xiaolin Huang21Xiaolin Huang22Xiaolin Huang23Qingjiang Station, Zhejiang Mariculture Research Institute, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaDivision of Culture and Food Processing Technology, Zhejiang Mariculture Research Institute, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaDivision of Culture and Food Processing Technology, Zhejiang Mariculture Research Institute, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaDivision of Culture and Food Processing Technology, Zhejiang Mariculture Research Institute, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaDivision of Culture and Food Processing Technology, Zhejiang Mariculture Research Institute, Wenzhou, ChinaQingjiang Station, Zhejiang Mariculture Research Institute, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaDivision of Culture and Food Processing Technology, Zhejiang Mariculture Research Institute, Wenzhou, ChinaZhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou, ChinaWenzhou key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, ChinaDivision of Culture and Food Processing Technology, Zhejiang Mariculture Research Institute, Wenzhou, ChinaIntroductionIn the context of Litopenaeus vannamei aquaculture, the incorporation of oyster shells has proven beneficial for enhancing water quality and the growth conditions of the shrimp. Nonetheless, the specific effects of in-situ water treatment using oyster shells on water quality and shrimp growth, along with the composition and succession dynamics of the microbial community within oyster shell biofilms, have yet to be thoroughly investigated.MethodsThis study established control, low-concentration, and high-concentration oyster shell addition groups to emulate the in-situ water treatment environment with oyster shells, with the objective of elucidating the impacts of oyster shell addition on the aquaculture setting.ResultsThe results showed that the addition of oyster shells could significantly improve the length (F = 12.248, P = 0.005), weight(F = 138.234, P < 0.001), and survival rate (F = 15.248, P < 0.001) of shrimp, while there were no significant differences in the length (F = -1.233, P = 0.267) and survival rate (F = -2.143, P = 0.076) between the high and low concentration groups. Additionally, oyster shell addition resulted in elevated phosphate levels (F = 74.92, P < 0.001 in Day 70), diminished nitrite levels (F = 5.276, P = 0.031 in Day 56), and increased nitrate concentrations (F = 9.421, P = 0.006 in Day 70). Within the biofilms, the relative abundances of Ruegeria, Tenacibaculum, BD2- 11_terrestrial_group, and Kapabacteriales exhibited significant declines over time, whereas the relative abundance of Nitrospira demonstrated a marked increase, ultimately emerging as the predominant bacterium (Relative abundance 31.8%) in the biofilms during the latter stages of the experiment. Nitrospira also exhibited a notably higher relative abundance in the microbial community of the experimental water group relative to the control group (F = 2.265, P = 0.001).DiscussionThe biofilm provided conditions for the proliferation of Nitrospira, thereby accelerating the transformation of nitrite into nitrate in the aquaculture system, which subsequently improved the shrimp farming conditions. This research offers valuable insights for the application of oyster shells in shrimp farming and contributes to the theoretical underpinnings necessary for advancing our understanding of the mechanisms through which oyster shell biofilms enhance water quality and foster shrimp health.https://www.frontiersin.org/articles/10.3389/fmars.2024.1495938/fullLitopenaeus vannameioyster shellsbiofilmmicrobial communitywater quality |
| spellingShingle | Xianke Huang Xianke Huang Xianke Huang Guoqiang Xiao Guoqiang Xiao Guoqiang Xiao Xiang Zhang Xiang Zhang Xiang Zhang Shuangshuang Teng Shuangshuang Teng Shuangshuang Teng Min Li Min Li Min Li Yilong Cai Yilong Cai Yilong Cai Ran Chen Ran Chen Ran Chen Xiaolin Huang Xiaolin Huang Xiaolin Huang Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities Frontiers in Marine Science Litopenaeus vannamei oyster shells biofilm microbial community water quality |
| title | Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities |
| title_full | Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities |
| title_fullStr | Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities |
| title_full_unstemmed | Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities |
| title_short | Effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities |
| title_sort | effects of oyster shell addition on shrimp aquaculture and the dynamic succession of surface biofilm microbial communities |
| topic | Litopenaeus vannamei oyster shells biofilm microbial community water quality |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2024.1495938/full |
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