Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens
Abstract Background High-fat diets (HFD) are known to enhance feed conversion ratio in broiler chickens, yet they can also result in hepatic fat accumulation. Bile acids (BAs) and gut microbiota also play key roles in the formation of fatty liver. In this study, our objective was to elucidate the me...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-08-01
|
| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-024-01071-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846165010367119360 |
|---|---|
| author | Minghui Wang Kelin Li Hongchao Jiao Jingpeng Zhao Haifang Li Yunlei Zhou Aizhi Cao Jianmin Wang Xiaojuan Wang Hai Lin |
| author_facet | Minghui Wang Kelin Li Hongchao Jiao Jingpeng Zhao Haifang Li Yunlei Zhou Aizhi Cao Jianmin Wang Xiaojuan Wang Hai Lin |
| author_sort | Minghui Wang |
| collection | DOAJ |
| description | Abstract Background High-fat diets (HFD) are known to enhance feed conversion ratio in broiler chickens, yet they can also result in hepatic fat accumulation. Bile acids (BAs) and gut microbiota also play key roles in the formation of fatty liver. In this study, our objective was to elucidate the mechanisms through which BA supplementation reduces hepatic fat deposition in broiler chickens, with a focus on the involvement of gut microbiota and liver BA composition. Results Newly hatched broiler chickens were allocated to either a low-fat diet (LFD) or HFD, supplemented with or without BAs, and subsequently assessed their impacts on gut microbiota, hepatic lipid metabolism, and hepatic BA composition. Our findings showed that BA supplementation significantly reduced plasma and liver tissue triglyceride (TG) levels in 42-day-old broiler chickens (P < 0.05), concurrently with a significant decrease in the expression levels of fatty acid synthase (FAS) in liver tissue (P < 0.05). These results suggest that BA supplementation effectively diminishes hepatic fat deposition. Under the LFD, BAs supplementation increased the BA content and ratio of Non 12-OH BAs/12-OH BAs in the liver and increased the Akkermansia abundance in cecum. Under the HFD, BA supplementation decreased the BAs and increased the relative abundances of chenodeoxycholic acid (CDCA) and cholic acid (CA) in hepatic tissue, while the relative abundances of Bacteroides were dramatically reduced and the Bifidobacterium, Escherichia, and Lactobacillus were increased in cecum. Correlation analyses showed a significant positive correlation between the Akkermansia abundance and Non 12-OH BA content under the LFD, and presented a significant negative correlation between the Bacteroides abundance and CA or CDCA content under the HFD. Conclusions The results indicate that supplementation of BAs in both LFD and HFD may ameliorate hepatic fat deposition in broiler chickens with the involvement of differentiated microbiota–bile acid profile pathways. Graphical Abstract |
| format | Article |
| id | doaj-art-e464a46e133947c182ab5e293d347b97 |
| institution | Kabale University |
| issn | 2049-1891 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj-art-e464a46e133947c182ab5e293d347b972024-11-17T12:40:03ZengBMCJournal of Animal Science and Biotechnology2049-18912024-08-0115112310.1186/s40104-024-01071-yDietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickensMinghui Wang0Kelin Li1Hongchao Jiao2Jingpeng Zhao3Haifang Li4Yunlei Zhou5Aizhi Cao6Jianmin Wang7Xiaojuan Wang8Hai Lin9College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural UniversityCollege of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural UniversityCollege of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural UniversityCollege of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Agricultural UniversityCollege of Chemistry and Material Science, Shandong Agricultural UniversityShandong Longchang Animal Health Products Co., Ltd.Shandong Longchang Animal Health Products Co., Ltd.College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural UniversityCollege of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural UniversityAbstract Background High-fat diets (HFD) are known to enhance feed conversion ratio in broiler chickens, yet they can also result in hepatic fat accumulation. Bile acids (BAs) and gut microbiota also play key roles in the formation of fatty liver. In this study, our objective was to elucidate the mechanisms through which BA supplementation reduces hepatic fat deposition in broiler chickens, with a focus on the involvement of gut microbiota and liver BA composition. Results Newly hatched broiler chickens were allocated to either a low-fat diet (LFD) or HFD, supplemented with or without BAs, and subsequently assessed their impacts on gut microbiota, hepatic lipid metabolism, and hepatic BA composition. Our findings showed that BA supplementation significantly reduced plasma and liver tissue triglyceride (TG) levels in 42-day-old broiler chickens (P < 0.05), concurrently with a significant decrease in the expression levels of fatty acid synthase (FAS) in liver tissue (P < 0.05). These results suggest that BA supplementation effectively diminishes hepatic fat deposition. Under the LFD, BAs supplementation increased the BA content and ratio of Non 12-OH BAs/12-OH BAs in the liver and increased the Akkermansia abundance in cecum. Under the HFD, BA supplementation decreased the BAs and increased the relative abundances of chenodeoxycholic acid (CDCA) and cholic acid (CA) in hepatic tissue, while the relative abundances of Bacteroides were dramatically reduced and the Bifidobacterium, Escherichia, and Lactobacillus were increased in cecum. Correlation analyses showed a significant positive correlation between the Akkermansia abundance and Non 12-OH BA content under the LFD, and presented a significant negative correlation between the Bacteroides abundance and CA or CDCA content under the HFD. Conclusions The results indicate that supplementation of BAs in both LFD and HFD may ameliorate hepatic fat deposition in broiler chickens with the involvement of differentiated microbiota–bile acid profile pathways. Graphical Abstracthttps://doi.org/10.1186/s40104-024-01071-yBile acidsBroiler chickensGut microbiotaHepatic fat depositionLiver bile acid profile |
| spellingShingle | Minghui Wang Kelin Li Hongchao Jiao Jingpeng Zhao Haifang Li Yunlei Zhou Aizhi Cao Jianmin Wang Xiaojuan Wang Hai Lin Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens Journal of Animal Science and Biotechnology Bile acids Broiler chickens Gut microbiota Hepatic fat deposition Liver bile acid profile |
| title | Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens |
| title_full | Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens |
| title_fullStr | Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens |
| title_full_unstemmed | Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens |
| title_short | Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens |
| title_sort | dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens |
| topic | Bile acids Broiler chickens Gut microbiota Hepatic fat deposition Liver bile acid profile |
| url | https://doi.org/10.1186/s40104-024-01071-y |
| work_keys_str_mv | AT minghuiwang dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT kelinli dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT hongchaojiao dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT jingpengzhao dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT haifangli dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT yunleizhou dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT aizhicao dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT jianminwang dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT xiaojuanwang dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens AT hailin dietarybileacidssupplementationdecreaseshepaticfatdepositionwiththeinvolvementofalteredgutmicrobiotaandliverbileacidsprofileinbroilerchickens |