Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates
Abstract Global health is affected by viral, bacterial, and fungal infections that cause chronic and often fatal diseases. Identifying novel antimicrobials through innovative methods that are active against human pathogens will create a new, necessary pipeline for chemical discovery and therapeutic...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-82056-9 |
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| author | Carolyn L. Fisher Hailey C. Loehde-Woolard Pamela D. Lane Catherine M. Mageeney Todd W. Lane |
| author_facet | Carolyn L. Fisher Hailey C. Loehde-Woolard Pamela D. Lane Catherine M. Mageeney Todd W. Lane |
| author_sort | Carolyn L. Fisher |
| collection | DOAJ |
| description | Abstract Global health is affected by viral, bacterial, and fungal infections that cause chronic and often fatal diseases. Identifying novel antimicrobials through innovative methods that are active against human pathogens will create a new, necessary pipeline for chemical discovery and therapeutic development. Our goal was to determine whether algal production systems represent fertile ground for discovery of antibiotics and antifungals. To this end, we collected high-biomass algal-bacterial samples from outdoor mass cultivation systems, 18-L outdoor algal open cultures mesocosms, and non-axenic laboratory samples. We also cultivated 33 marine bacterial isolates for chemical extraction. Ultimately, we filtered, concentrated, extracted, and screened 77 chemically-complex mixtures using a conventional agar-based microbial growth inhibition assay against three microbes: Escherichia coli, Bacillus subtilis, and Candida albicans. We discovered that 23 of our chemical extracts (almost one-third of the chemical samples tested) exhibited some degree of growth inhibition toward B. subtilis and/or C. albicans. Our work here demonstrates the feasibility and potential of isolating bioactive natural products from high-biomass algal-bacterial samples from algal mass cultivation systems. |
| format | Article |
| id | doaj-art-e4649b003afb4b2fa3905dc6a68bc734 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e4649b003afb4b2fa3905dc6a68bc7342025-01-05T12:28:06ZengNature PortfolioScientific Reports2045-23222024-12-011411910.1038/s41598-024-82056-9Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolatesCarolyn L. Fisher0Hailey C. Loehde-Woolard1Pamela D. Lane2Catherine M. Mageeney3Todd W. Lane4Physical and Life Science Directorate, Bioscience & BioTechnology Division, Lawrence Livermore National LaboratoryDepartment of Chemical and Biological Engineering, University of ColoradoSystems Biology, Sandia National LaboratoriesBiotechnology and Bioengineering, Sandia National LaboratoriesBioresource and Environmental Security, Sandia National LaboratoriesAbstract Global health is affected by viral, bacterial, and fungal infections that cause chronic and often fatal diseases. Identifying novel antimicrobials through innovative methods that are active against human pathogens will create a new, necessary pipeline for chemical discovery and therapeutic development. Our goal was to determine whether algal production systems represent fertile ground for discovery of antibiotics and antifungals. To this end, we collected high-biomass algal-bacterial samples from outdoor mass cultivation systems, 18-L outdoor algal open cultures mesocosms, and non-axenic laboratory samples. We also cultivated 33 marine bacterial isolates for chemical extraction. Ultimately, we filtered, concentrated, extracted, and screened 77 chemically-complex mixtures using a conventional agar-based microbial growth inhibition assay against three microbes: Escherichia coli, Bacillus subtilis, and Candida albicans. We discovered that 23 of our chemical extracts (almost one-third of the chemical samples tested) exhibited some degree of growth inhibition toward B. subtilis and/or C. albicans. Our work here demonstrates the feasibility and potential of isolating bioactive natural products from high-biomass algal-bacterial samples from algal mass cultivation systems.https://doi.org/10.1038/s41598-024-82056-9AntimicrobialAntifungalAlgal production systemsAlgal mass cultivation systemsMicrobial consortiaNatural products |
| spellingShingle | Carolyn L. Fisher Hailey C. Loehde-Woolard Pamela D. Lane Catherine M. Mageeney Todd W. Lane Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates Scientific Reports Antimicrobial Antifungal Algal production systems Algal mass cultivation systems Microbial consortia Natural products |
| title | Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates |
| title_full | Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates |
| title_fullStr | Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates |
| title_full_unstemmed | Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates |
| title_short | Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates |
| title_sort | discovery of antimicrobial activity in chemical extracts derived from unexplored algal bacterial culture systems and isolates |
| topic | Antimicrobial Antifungal Algal production systems Algal mass cultivation systems Microbial consortia Natural products |
| url | https://doi.org/10.1038/s41598-024-82056-9 |
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