β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats
Abstract Arthropathy is a common complication in haemophilia and decreases quality of life. It has been known that concentrations of β-hydroxybutyrate (BHB) in blood are increased by a ketogenic diet, and elevated levels of circulating BHB restricts the progression of inflammation-mediated joint pat...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-77074-6 |
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| author | Ryohei Kawasaki Asuka Sakata Kohei Tatsumi Seiji Mitani Maiko Takeda Shogo Kasuda Naoki Matsumoto Suguru Harada Tetsuhiro Soeda Yukiko Nishida Yasushi Yoshimura Midori Shima |
| author_facet | Ryohei Kawasaki Asuka Sakata Kohei Tatsumi Seiji Mitani Maiko Takeda Shogo Kasuda Naoki Matsumoto Suguru Harada Tetsuhiro Soeda Yukiko Nishida Yasushi Yoshimura Midori Shima |
| author_sort | Ryohei Kawasaki |
| collection | DOAJ |
| description | Abstract Arthropathy is a common complication in haemophilia and decreases quality of life. It has been known that concentrations of β-hydroxybutyrate (BHB) in blood are increased by a ketogenic diet, and elevated levels of circulating BHB restricts the progression of inflammation-mediated joint pathological changes. We hypothesized that elevation of blood BHB concentrations could be effective for reducing the progression of bleeding-induced arthropathy by moderating the inflammatory responses of macrophages. In this study, we investigated whether BHB alleviates the arthropathy caused by repeated intra-articular blood injection in rats. To increase blood BHB levels, rats were fed with ketogenic diet. Repeated intra-articular blood injection induced significant joint swelling, whereas ketogenic diet intake significantly increased blood BHB concentrations and ameliorated the joint swelling. The periarticular tissue-fibrosis observed in the control diet intake group appeared to be significantly alleviated in the ketogenic diet intake group. In addition, the IL-1β, which is involved in the progression of arthropathy, levels in the supernatants of blood-exposed macrophages derived from THP-1 cell line were significantly suppressed by BHB supplementation. In summary, BHB moderated the pathological joint changes caused by intra-articular blood exposure. |
| format | Article |
| id | doaj-art-e4317ddda0cd4c68b9459e14c15cdd6d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-e4317ddda0cd4c68b9459e14c15cdd6d2024-12-01T12:20:50ZengNature PortfolioScientific Reports2045-23222024-11-0114111210.1038/s41598-024-77074-6β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in ratsRyohei Kawasaki0Asuka Sakata1Kohei Tatsumi2Seiji Mitani3Maiko Takeda4Shogo Kasuda5Naoki Matsumoto6Suguru Harada7Tetsuhiro Soeda8Yukiko Nishida9Yasushi Yoshimura10Midori Shima11Medicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityAdvanced Medical Science of Thrombosis and Hemostasis, Nara Medical UniversityDepartment of Diagnostic Pathology, Nara Medical UniversityDepartment of Legal Medicine, Nara Medical UniversityMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityProduct Research Department, Medical Affairs Division, Chugai Pharmaceutical Co., LtdMedicinal Biology of Thrombosis and Hemostasis, Nara Medical UniversityAbstract Arthropathy is a common complication in haemophilia and decreases quality of life. It has been known that concentrations of β-hydroxybutyrate (BHB) in blood are increased by a ketogenic diet, and elevated levels of circulating BHB restricts the progression of inflammation-mediated joint pathological changes. We hypothesized that elevation of blood BHB concentrations could be effective for reducing the progression of bleeding-induced arthropathy by moderating the inflammatory responses of macrophages. In this study, we investigated whether BHB alleviates the arthropathy caused by repeated intra-articular blood injection in rats. To increase blood BHB levels, rats were fed with ketogenic diet. Repeated intra-articular blood injection induced significant joint swelling, whereas ketogenic diet intake significantly increased blood BHB concentrations and ameliorated the joint swelling. The periarticular tissue-fibrosis observed in the control diet intake group appeared to be significantly alleviated in the ketogenic diet intake group. In addition, the IL-1β, which is involved in the progression of arthropathy, levels in the supernatants of blood-exposed macrophages derived from THP-1 cell line were significantly suppressed by BHB supplementation. In summary, BHB moderated the pathological joint changes caused by intra-articular blood exposure.https://doi.org/10.1038/s41598-024-77074-6 |
| spellingShingle | Ryohei Kawasaki Asuka Sakata Kohei Tatsumi Seiji Mitani Maiko Takeda Shogo Kasuda Naoki Matsumoto Suguru Harada Tetsuhiro Soeda Yukiko Nishida Yasushi Yoshimura Midori Shima β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats Scientific Reports |
| title | β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats |
| title_full | β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats |
| title_fullStr | β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats |
| title_full_unstemmed | β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats |
| title_short | β-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats |
| title_sort | β hydroxybutyrate suppresses pathological changes of blood induced arthropathy in rats |
| url | https://doi.org/10.1038/s41598-024-77074-6 |
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