Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells
Background and Objectives Air pollution, particularly particulate matter (PM), has a variety of adverse effects on human health. PM is known to induce cell death through various pathways, including pyroptosis. Despite its significance, research on PM-induced pyroptosis in nasal epithelial cells rema...
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Korean Rhinologic Society
2024-07-01
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| Series: | Journal of Rhinology |
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| Online Access: | http://j-rhinology.org/upload/pdf/jr-2024-00021.pdf |
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| author | Hosung Choi Hyunsu Choi Jeong-Min Oh Dong Chang Lee |
| author_facet | Hosung Choi Hyunsu Choi Jeong-Min Oh Dong Chang Lee |
| author_sort | Hosung Choi |
| collection | DOAJ |
| description | Background and Objectives Air pollution, particularly particulate matter (PM), has a variety of adverse effects on human health. PM is known to induce cell death through various pathways, including pyroptosis. Despite its significance, research on PM-induced pyroptosis in nasal epithelial cells remains limited. This study aimed to explore PM-induced pyroptosis in cultured human nasal epithelial cells. Methods For the in vitro experiments, human nasal epithelial cells were cultured. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, while cell death was evaluated through propidium iodide (PI) staining and lactate dehydrogenase (LDH) release measurement. Protein expression levels related to pyroptosis were examined via western blot using antibodies against NOD-like receptor family, pyrin domain containing 3 (NLRP3), cleaved caspase-1 (CASP1 P20), gasdermin D (GSDMD)-N, and glyceraldehyde phosphate dehydrogenase. Immunofluorescent staining with a CASP1 P20 antibody was conducted to visualize cellular localization. Enzyme-linked immunosorbent assay was utilized to quantify interleukin (IL)-1β and IL-18 protein levels. Results Treatment with PM resulted in decreased cell viability, elevated LDH release, and intensified PI staining, indicating cell death. Pyroptosis was confirmed by the elevated expression of NLRP3, CASP1 P20, and GSDMD-N, along with increased levels of IL-1β and IL-18. Inhibiting the NLRP3 inflammasome with MCC950 reduced the PM-induced effects on protein expression and cytokine release, highlighting the role of the NLRP3 inflammasome in PM-triggered pyroptosis in human nasal epithelial cells. Conclusion We showed that PM triggers pyroptosis in human nasal epithelial cells, driven by NLRP3 inflammasome-dependent signaling pathways. |
| format | Article |
| id | doaj-art-e3ca1d3d20bc441d9ad442b2bcbeb76a |
| institution | Kabale University |
| issn | 1229-1498 2384-4361 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Korean Rhinologic Society |
| record_format | Article |
| series | Journal of Rhinology |
| spelling | doaj-art-e3ca1d3d20bc441d9ad442b2bcbeb76a2024-11-18T07:29:24ZengKorean Rhinologic SocietyJournal of Rhinology1229-14982384-43612024-07-0131210611310.18787/jr.2024.00021814Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial CellsHosung Choi0Hyunsu Choi1Jeong-Min Oh2Dong Chang Lee3 Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea Clinical Research Institute, Daejeon St. Mary’s Hospital, Daejeon, Republic of Korea Clinical Research Institute, Daejeon St. Mary’s Hospital, Daejeon, Republic of Korea Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaBackground and Objectives Air pollution, particularly particulate matter (PM), has a variety of adverse effects on human health. PM is known to induce cell death through various pathways, including pyroptosis. Despite its significance, research on PM-induced pyroptosis in nasal epithelial cells remains limited. This study aimed to explore PM-induced pyroptosis in cultured human nasal epithelial cells. Methods For the in vitro experiments, human nasal epithelial cells were cultured. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, while cell death was evaluated through propidium iodide (PI) staining and lactate dehydrogenase (LDH) release measurement. Protein expression levels related to pyroptosis were examined via western blot using antibodies against NOD-like receptor family, pyrin domain containing 3 (NLRP3), cleaved caspase-1 (CASP1 P20), gasdermin D (GSDMD)-N, and glyceraldehyde phosphate dehydrogenase. Immunofluorescent staining with a CASP1 P20 antibody was conducted to visualize cellular localization. Enzyme-linked immunosorbent assay was utilized to quantify interleukin (IL)-1β and IL-18 protein levels. Results Treatment with PM resulted in decreased cell viability, elevated LDH release, and intensified PI staining, indicating cell death. Pyroptosis was confirmed by the elevated expression of NLRP3, CASP1 P20, and GSDMD-N, along with increased levels of IL-1β and IL-18. Inhibiting the NLRP3 inflammasome with MCC950 reduced the PM-induced effects on protein expression and cytokine release, highlighting the role of the NLRP3 inflammasome in PM-triggered pyroptosis in human nasal epithelial cells. Conclusion We showed that PM triggers pyroptosis in human nasal epithelial cells, driven by NLRP3 inflammasome-dependent signaling pathways.http://j-rhinology.org/upload/pdf/jr-2024-00021.pdfparticulate matterpyroptosisinflammasomesnasal mucosa |
| spellingShingle | Hosung Choi Hyunsu Choi Jeong-Min Oh Dong Chang Lee Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells Journal of Rhinology particulate matter pyroptosis inflammasomes nasal mucosa |
| title | Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells |
| title_full | Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells |
| title_fullStr | Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells |
| title_full_unstemmed | Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells |
| title_short | Particulate Matter Induces NLRP3 Inflammasome-Mediated Pyroptosis in Human Nasal Epithelial Cells |
| title_sort | particulate matter induces nlrp3 inflammasome mediated pyroptosis in human nasal epithelial cells |
| topic | particulate matter pyroptosis inflammasomes nasal mucosa |
| url | http://j-rhinology.org/upload/pdf/jr-2024-00021.pdf |
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