Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression
Ginger is a spice and medicinal plant with several varieties. This study aimed to understand the antioxidant, antidiabetic, and antiobesity properties of red ginger (RG) (Zingiber officinale var. rubrum), through pharmacoinformatics coupled with in vitro studies. Additionally, the suppression of miR...
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324005635 |
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| author | Derren David Christian Homenta Rampengan Rony Abdi Syahputra Princella Halim Dian Aruni Kumalawati Roy Novri Ramadhan Reggie Surya Elvan Wiyarta Happy Kurnia Permatasari Raymond Rubianto Tjandrawinata Nurpudji Astuti Taslim Bonglee Kim Trina Ekawati Tallei Apollinaire Tsopmo Fahrul Nurkolis |
| author_facet | Derren David Christian Homenta Rampengan Rony Abdi Syahputra Princella Halim Dian Aruni Kumalawati Roy Novri Ramadhan Reggie Surya Elvan Wiyarta Happy Kurnia Permatasari Raymond Rubianto Tjandrawinata Nurpudji Astuti Taslim Bonglee Kim Trina Ekawati Tallei Apollinaire Tsopmo Fahrul Nurkolis |
| author_sort | Derren David Christian Homenta Rampengan |
| collection | DOAJ |
| description | Ginger is a spice and medicinal plant with several varieties. This study aimed to understand the antioxidant, antidiabetic, and antiobesity properties of red ginger (RG) (Zingiber officinale var. rubrum), through pharmacoinformatics coupled with in vitro studies. Additionally, the suppression of miR-21/132 expression by RG was studied. Two RG extracts were sequentially produced using hexane (RGH) and ethanol (RGE) and characterized using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics analysis. Seven compounds identified in RGE and six in RGH were subjected to molecular docking tests on iNOS, lipase, α-glucosidase, α-amylase, and FTO protein receptors. Overall, 5,7-dihydroxy-2-(4-hydroxyphenyl)-6,8-bis(3,4,5-trihydroxyoxan-2-yl)-4H-chromen-4-one and pheophorbide A from RGE, and nictoflorin and rutin from RGH showed superior binding to most receptors. In vitro studies confirmed the ability of both RGE and RGH extracts to scavenge DPPH and ABTS radicals; inhibit activities of three metabolic enzymes, lipase (EC50 85.58 and 105.50 μg/mL), α-glucosidase (EC50 of 92.56 and 106.20 μg/mL), and α-amylase (EC50 of 96.60 and 111.80 μg/mL). Ex vivo RGE and RGH considerably suppressed protein expression associated with obesity, diabetes, and oxidative stress, including miR-21/132. This presents new insights into the molecular mechanism of RG in combating metabolic syndrome; however, further in vivo and clinical trials are needed to validate these findings. |
| format | Article |
| id | doaj-art-e3c68958555940c7bb350e5baaac9a83 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-e3c68958555940c7bb350e5baaac9a832024-12-16T05:38:12ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-0118101526Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expressionDerren David Christian Homenta Rampengan0Rony Abdi Syahputra1Princella Halim2Dian Aruni Kumalawati3Roy Novri Ramadhan4Reggie Surya5Elvan Wiyarta6Happy Kurnia Permatasari7Raymond Rubianto Tjandrawinata8Nurpudji Astuti Taslim9Bonglee Kim10Trina Ekawati Tallei11Apollinaire Tsopmo12Fahrul Nurkolis13Medical Study Programme, Faculty of Medicine, Universitas Sam Ratulangi, Manado, IndonesiaDepartment of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia; Corresponding author.Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara, IndonesiaDepartment of Biomedical Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta, 55281, IndonesiaMedical Study Programme, Faculty of Medicine, Airlangga University, Surabaya, IndonesiaDepartment of Food Technology, Faculty of Engineering, Bina Nusantara University, Jakarta, 11480, IndonesiaDepartment of Neurology, Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo National 13 Hospital, Jakarta, 10430, IndonesiaDepartment of Biochemistry and Biomolecular, Faculty of Medicine, University of Brawijaya, Malang, 65145, IndonesiaCenter for Pharmaceutical and Nutraceutical Research and Policy, Atma Jaya Catholic University of Indonesia, Jakarta, 12930, IndonesiaDivision of Clinical Nutrition, Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, 90245, IndonesiaDepartment of Pathology, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea; Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of KoreaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Manado, 95115, IndonesiaFood Science and Nutrition Program, Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, CanadaMedical Research Center of Indonesia, Surabaya, IndonesiaGinger is a spice and medicinal plant with several varieties. This study aimed to understand the antioxidant, antidiabetic, and antiobesity properties of red ginger (RG) (Zingiber officinale var. rubrum), through pharmacoinformatics coupled with in vitro studies. Additionally, the suppression of miR-21/132 expression by RG was studied. Two RG extracts were sequentially produced using hexane (RGH) and ethanol (RGE) and characterized using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics analysis. Seven compounds identified in RGE and six in RGH were subjected to molecular docking tests on iNOS, lipase, α-glucosidase, α-amylase, and FTO protein receptors. Overall, 5,7-dihydroxy-2-(4-hydroxyphenyl)-6,8-bis(3,4,5-trihydroxyoxan-2-yl)-4H-chromen-4-one and pheophorbide A from RGE, and nictoflorin and rutin from RGH showed superior binding to most receptors. In vitro studies confirmed the ability of both RGE and RGH extracts to scavenge DPPH and ABTS radicals; inhibit activities of three metabolic enzymes, lipase (EC50 85.58 and 105.50 μg/mL), α-glucosidase (EC50 of 92.56 and 106.20 μg/mL), and α-amylase (EC50 of 96.60 and 111.80 μg/mL). Ex vivo RGE and RGH considerably suppressed protein expression associated with obesity, diabetes, and oxidative stress, including miR-21/132. This presents new insights into the molecular mechanism of RG in combating metabolic syndrome; however, further in vivo and clinical trials are needed to validate these findings.http://www.sciencedirect.com/science/article/pii/S2666154324005635AntidiabetesAntiobesityantioxidantMetabolic syndromeMolecular mechanismred ginger |
| spellingShingle | Derren David Christian Homenta Rampengan Rony Abdi Syahputra Princella Halim Dian Aruni Kumalawati Roy Novri Ramadhan Reggie Surya Elvan Wiyarta Happy Kurnia Permatasari Raymond Rubianto Tjandrawinata Nurpudji Astuti Taslim Bonglee Kim Trina Ekawati Tallei Apollinaire Tsopmo Fahrul Nurkolis Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression Journal of Agriculture and Food Research Antidiabetes Antiobesity antioxidant Metabolic syndrome Molecular mechanism red ginger |
| title | Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression |
| title_full | Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression |
| title_fullStr | Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression |
| title_full_unstemmed | Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression |
| title_short | Red ginger confers antioxidant activity, inhibits lipid and sugar metabolic enzymes, and downregulates miR-21/132 expression |
| title_sort | red ginger confers antioxidant activity inhibits lipid and sugar metabolic enzymes and downregulates mir 21 132 expression |
| topic | Antidiabetes Antiobesity antioxidant Metabolic syndrome Molecular mechanism red ginger |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324005635 |
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