A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)

A new stackable modular system was developed for continuous in vivo production of phytoseiid mites. The system consists of cage units that are filled with lima beans,  Phaseolus lunatus, or red beans, P. vulgaris, leaves infested with high levels of the two-spotted spider mites, Tetranychus urticae....

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Main Authors: Juan Alfredo Morales-Ramos, Maria Guadalupe Rojas
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Psyche: A Journal of Entomology
Online Access:http://dx.doi.org/10.1155/2014/596768
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author Juan Alfredo Morales-Ramos
Maria Guadalupe Rojas
author_facet Juan Alfredo Morales-Ramos
Maria Guadalupe Rojas
author_sort Juan Alfredo Morales-Ramos
collection DOAJ
description A new stackable modular system was developed for continuous in vivo production of phytoseiid mites. The system consists of cage units that are filled with lima beans,  Phaseolus lunatus, or red beans, P. vulgaris, leaves infested with high levels of the two-spotted spider mites, Tetranychus urticae. The cage units connect with each other through a connection cup, which also serves for monitoring and collection. Predatory mites migrate upwards to new cage units as prey is depleted. The system was evaluated for production of Phytoseiulus persimilis. During a 6-month experimental period, 20,894.9±10,482.5 (mean ± standard deviation) predators were produced per week. The production consisted of 4.1±4.6% nymphs and 95.9±4.6% adults. A mean of 554.5±59.8 predatory mites were collected per harvested cage and the mean interval length between harvests was 6.57±6.76 days. The potential for commercial and experimental applications is discussed.
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language English
publishDate 2014-01-01
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series Psyche: A Journal of Entomology
spelling doaj-art-e3ac4ce9b40e44c88097e143cc991e232025-02-03T05:47:35ZengWileyPsyche: A Journal of Entomology0033-26151687-74382014-01-01201410.1155/2014/596768596768A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)Juan Alfredo Morales-Ramos0Maria Guadalupe Rojas1USDA-ARS National Biological Control Laboratory, Biological Control of Pests Research Unit, 59 Lee Road, Stoneville, MS 38776, USAUSDA-ARS National Biological Control Laboratory, Biological Control of Pests Research Unit, 59 Lee Road, Stoneville, MS 38776, USAA new stackable modular system was developed for continuous in vivo production of phytoseiid mites. The system consists of cage units that are filled with lima beans,  Phaseolus lunatus, or red beans, P. vulgaris, leaves infested with high levels of the two-spotted spider mites, Tetranychus urticae. The cage units connect with each other through a connection cup, which also serves for monitoring and collection. Predatory mites migrate upwards to new cage units as prey is depleted. The system was evaluated for production of Phytoseiulus persimilis. During a 6-month experimental period, 20,894.9±10,482.5 (mean ± standard deviation) predators were produced per week. The production consisted of 4.1±4.6% nymphs and 95.9±4.6% adults. A mean of 554.5±59.8 predatory mites were collected per harvested cage and the mean interval length between harvests was 6.57±6.76 days. The potential for commercial and experimental applications is discussed.http://dx.doi.org/10.1155/2014/596768
spellingShingle Juan Alfredo Morales-Ramos
Maria Guadalupe Rojas
A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)
Psyche: A Journal of Entomology
title A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)
title_full A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)
title_fullStr A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)
title_full_unstemmed A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)
title_short A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)
title_sort modular cage system design for continuous medium to large scale in vivo rearing of predatory mites acari phytoseiidae
url http://dx.doi.org/10.1155/2014/596768
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