Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method
Lens-free on-chip microscopy (LFOCM) is a powerful computational imaging technology that combines high-throughput capabilities with cost efficiency. However, in LFOCM, the phase recovered by iterative phase retrieval techniques is generally wrapped into the range of −<inline-formula><math x...
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2024-12-01
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| author | Yufan Chen Xuejuan Wu Yang Chen Wenhui Lin Haojie Gu Yuzhen Zhang Chao Zuo |
| author_facet | Yufan Chen Xuejuan Wu Yang Chen Wenhui Lin Haojie Gu Yuzhen Zhang Chao Zuo |
| author_sort | Yufan Chen |
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| description | Lens-free on-chip microscopy (LFOCM) is a powerful computational imaging technology that combines high-throughput capabilities with cost efficiency. However, in LFOCM, the phase recovered by iterative phase retrieval techniques is generally wrapped into the range of −<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>π</mi></semantics></math></inline-formula> to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>π</mi></semantics></math></inline-formula>, necessitating phase unwrapping to recover absolute phase distributions. Moreover, this unwrapping process is prone to errors, particularly in areas with large phase gradients or low spatial sampling, due to the absence of reliable initial guesses. To address these challenges, we propose a novel biplane phase retrieval (BPR) method that integrates phase unwrapping results obtained at different propagation distances to achieve accurate absolute phase reconstruction. The effectiveness of BPR is validated through live-cell imaging of HeLa cells, demonstrating improved quantitative phase imaging (QPI) accuracy when compared to conventional off-axis digital holographic microscopy. Furthermore, time-lapse imaging of COS-7 cells in vitro highlights the method’s robustness and capability for long-term quantitative analysis of large cell populations. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-e3702f5fc05a40eeb3f7c0558f334d4a2025-01-10T13:20:29ZengMDPI AGSensors1424-82202024-12-01251310.3390/s25010003Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval MethodYufan Chen0Xuejuan Wu1Yang Chen2Wenhui Lin3Haojie Gu4Yuzhen Zhang5Chao Zuo6Smart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSmart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSmart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSmart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSmart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSmart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaSmart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaLens-free on-chip microscopy (LFOCM) is a powerful computational imaging technology that combines high-throughput capabilities with cost efficiency. However, in LFOCM, the phase recovered by iterative phase retrieval techniques is generally wrapped into the range of −<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>π</mi></semantics></math></inline-formula> to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>π</mi></semantics></math></inline-formula>, necessitating phase unwrapping to recover absolute phase distributions. Moreover, this unwrapping process is prone to errors, particularly in areas with large phase gradients or low spatial sampling, due to the absence of reliable initial guesses. To address these challenges, we propose a novel biplane phase retrieval (BPR) method that integrates phase unwrapping results obtained at different propagation distances to achieve accurate absolute phase reconstruction. The effectiveness of BPR is validated through live-cell imaging of HeLa cells, demonstrating improved quantitative phase imaging (QPI) accuracy when compared to conventional off-axis digital holographic microscopy. Furthermore, time-lapse imaging of COS-7 cells in vitro highlights the method’s robustness and capability for long-term quantitative analysis of large cell populations.https://www.mdpi.com/1424-8220/25/1/3lens-free on-chip microscopyquantitative phase imagingbiplane phase retrievalhigh-throughput imagingiterative phase retrieval |
| spellingShingle | Yufan Chen Xuejuan Wu Yang Chen Wenhui Lin Haojie Gu Yuzhen Zhang Chao Zuo Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method Sensors lens-free on-chip microscopy quantitative phase imaging biplane phase retrieval high-throughput imaging iterative phase retrieval |
| title | Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method |
| title_full | Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method |
| title_fullStr | Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method |
| title_full_unstemmed | Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method |
| title_short | Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method |
| title_sort | lens free on chip quantitative phase microscopy for large phase objects based on a biplane phase retrieval method |
| topic | lens-free on-chip microscopy quantitative phase imaging biplane phase retrieval high-throughput imaging iterative phase retrieval |
| url | https://www.mdpi.com/1424-8220/25/1/3 |
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