Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis
This study examined the diversity and responses of intestinal microbiota in hybrid grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) fed diets with varying levels of fishmeal replaced by black soldier fly larvae (BSFL). The 10% BSFL substitution (BSFL10)...
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2024-12-01
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| author | Yan Chen Jun Ma Yoong-Soon Yong Yonggan Chen Bing Chen Junming Cao Kai Peng Guaxia Wang Hai Huang Jiun-Yan Loh |
| author_facet | Yan Chen Jun Ma Yoong-Soon Yong Yonggan Chen Bing Chen Junming Cao Kai Peng Guaxia Wang Hai Huang Jiun-Yan Loh |
| author_sort | Yan Chen |
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| description | This study examined the diversity and responses of intestinal microbiota in hybrid grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) fed diets with varying levels of fishmeal replaced by black soldier fly larvae (BSFL). The 10% BSFL substitution (BSFL10) group showed the highest levels of trypsin and amylase. Substituting fishmeal with 30% and 50% BSFL weakened the intestinal wall, resulting in vacuoles, sparse striatal boundaries, and fewer villi. Microbiota diversity, measured through Shannon’s index, was higher in the BSFL10 and BSFL50 groups than in the control. 16S rRNA amplicon data revealed the dominance of Firmicutes, Proteobacteria, Bacteroidetes, Spirochaetota, and Verrucomicrobia phyla. The BSFL-replacement groups showed an increase in Proteobacteria, Bacteroidetes, and Spirochaetota compared to the control, but fewer Firmicutes. PICRUSt analysis indicated significant alterations in microbial function, particularly enhanced protein, carbohydrate, lipid, and energy metabolisms in the BSFL-fed group. Substituting 10% fishmeal with BSFL enhanced nutrient metabolism and gut microbiota in juvenile hybrid grouper. Further research is needed to explore factors affecting the efficacy of insect feed as a sustainable aquaculture diet. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-e3659b56a3754384a9a2275f0d0d5dd92024-12-27T14:05:36ZengMDPI AGAnimals2076-26152024-12-011424359610.3390/ani14243596Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive AnalysisYan Chen0Jun Ma1Yoong-Soon Yong2Yonggan Chen3Bing Chen4Junming Cao5Kai Peng6Guaxia Wang7Hai Huang8Jiun-Yan Loh9Yazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572024, ChinaYazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572024, ChinaR&D Quality Department, Osmosis Nutrition Sdn Bhd, Bandar Nilai Utama, Nilai 71800, Negeri Sembilan, MalaysiaYazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572024, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Collaborative Innovation Center of Aquatic Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Collaborative Innovation Center of Aquatic Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Collaborative Innovation Center of Aquatic Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Collaborative Innovation Center of Aquatic Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaYazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572024, ChinaTropical Futures Institute, James Cook University Singapore, 149 Sims Drive, Singapore 387380, SingaporeThis study examined the diversity and responses of intestinal microbiota in hybrid grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) fed diets with varying levels of fishmeal replaced by black soldier fly larvae (BSFL). The 10% BSFL substitution (BSFL10) group showed the highest levels of trypsin and amylase. Substituting fishmeal with 30% and 50% BSFL weakened the intestinal wall, resulting in vacuoles, sparse striatal boundaries, and fewer villi. Microbiota diversity, measured through Shannon’s index, was higher in the BSFL10 and BSFL50 groups than in the control. 16S rRNA amplicon data revealed the dominance of Firmicutes, Proteobacteria, Bacteroidetes, Spirochaetota, and Verrucomicrobia phyla. The BSFL-replacement groups showed an increase in Proteobacteria, Bacteroidetes, and Spirochaetota compared to the control, but fewer Firmicutes. PICRUSt analysis indicated significant alterations in microbial function, particularly enhanced protein, carbohydrate, lipid, and energy metabolisms in the BSFL-fed group. Substituting 10% fishmeal with BSFL enhanced nutrient metabolism and gut microbiota in juvenile hybrid grouper. Further research is needed to explore factors affecting the efficacy of insect feed as a sustainable aquaculture diet.https://www.mdpi.com/2076-2615/14/24/3596black soldier flyenzymeshistopathologyhybrid grouperintestinal microbiotasustainable aquaculture |
| spellingShingle | Yan Chen Jun Ma Yoong-Soon Yong Yonggan Chen Bing Chen Junming Cao Kai Peng Guaxia Wang Hai Huang Jiun-Yan Loh Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis Animals black soldier fly enzymes histopathology hybrid grouper intestinal microbiota sustainable aquaculture |
| title | Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis |
| title_full | Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis |
| title_fullStr | Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis |
| title_full_unstemmed | Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis |
| title_short | Impacts of Black Soldier Fly (<i>Hermetia illucens</i>) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂): A Comprehensive Analysis |
| title_sort | impacts of black soldier fly i hermetia illucens i larval meal on intestinal histopathology and microbiome responses in hybrid grouper i epinephelus fuscoguttatus i ♀ i e lanceolatus i ♂ a comprehensive analysis |
| topic | black soldier fly enzymes histopathology hybrid grouper intestinal microbiota sustainable aquaculture |
| url | https://www.mdpi.com/2076-2615/14/24/3596 |
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