Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach
Abstract Articular cartilage defects present a significant therapeutic challenge due to the inherent avascular and aneural characteristics of cartilage tissue. Gene therapy has emerged as a promising strategy for cartilage regeneration, particularly through the use of functional RNA and biomaterial‐...
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202406479 |
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| author | Wei Zhu Han Wang Bin Feng Guangli Liu Yixin Bian Tianhao Zhao Qi Wang Xisheng Weng |
| author_facet | Wei Zhu Han Wang Bin Feng Guangli Liu Yixin Bian Tianhao Zhao Qi Wang Xisheng Weng |
| author_sort | Wei Zhu |
| collection | DOAJ |
| description | Abstract Articular cartilage defects present a significant therapeutic challenge due to the inherent avascular and aneural characteristics of cartilage tissue. Gene therapy has emerged as a promising strategy for cartilage regeneration, particularly through the use of functional RNA and biomaterial‐assisted frameworks. In this study, an innovative gene‐activated self‐healing hydrogel is developed and fabricated for the controlled release of miR140‐5p, a key regulator of cartilage regeneration. The hydrogel, crosslinked via UV radiation, is composed of aminated hyaluronic acid and a modified photosensitizer (NB). To enhance the scaffold's structural integrity and gene delivery efficiency, mineralized silk fibroin and miR140‐5p‐loaded MON‐PEI nanoparticles are incorporated. These findings demonstrate that this novel hydrogel (miR140‐5p‐CaP@mSF‐HA‐NB) effectively encapsulates and releases miR140‐5p, exhibits excellent biocompatibility, and promotes enhanced cartilage regeneration in both in vitro and in vivo models. Therefore, this gene‐activated hydrogel holds significant potential for clinical applications in the treatment of articular cartilage defects. |
| format | Article |
| id | doaj-art-e348a6a7921c4065b1d60b91ea5a5da9 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-e348a6a7921c4065b1d60b91ea5a5da92025-01-09T11:44:45ZengWileyAdvanced Science2198-38442025-01-01121n/an/a10.1002/advs.202406479Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly ApproachWei Zhu0Han Wang1Bin Feng2Guangli Liu3Yixin Bian4Tianhao Zhao5Qi Wang6Xisheng Weng7Department of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedics State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 ChinaAbstract Articular cartilage defects present a significant therapeutic challenge due to the inherent avascular and aneural characteristics of cartilage tissue. Gene therapy has emerged as a promising strategy for cartilage regeneration, particularly through the use of functional RNA and biomaterial‐assisted frameworks. In this study, an innovative gene‐activated self‐healing hydrogel is developed and fabricated for the controlled release of miR140‐5p, a key regulator of cartilage regeneration. The hydrogel, crosslinked via UV radiation, is composed of aminated hyaluronic acid and a modified photosensitizer (NB). To enhance the scaffold's structural integrity and gene delivery efficiency, mineralized silk fibroin and miR140‐5p‐loaded MON‐PEI nanoparticles are incorporated. These findings demonstrate that this novel hydrogel (miR140‐5p‐CaP@mSF‐HA‐NB) effectively encapsulates and releases miR140‐5p, exhibits excellent biocompatibility, and promotes enhanced cartilage regeneration in both in vitro and in vivo models. Therefore, this gene‐activated hydrogel holds significant potential for clinical applications in the treatment of articular cartilage defects.https://doi.org/10.1002/advs.202406479chondrocyte regenerationhydrogelmiRNA140–5pself‐healing |
| spellingShingle | Wei Zhu Han Wang Bin Feng Guangli Liu Yixin Bian Tianhao Zhao Qi Wang Xisheng Weng Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach Advanced Science chondrocyte regeneration hydrogel miRNA140–5p self‐healing |
| title | Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach |
| title_full | Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach |
| title_fullStr | Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach |
| title_full_unstemmed | Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach |
| title_short | Self‐Healing Hyaluronic Acid‐based Hydrogel with miRNA140‐5p Loaded MON‐PEI Nanoparticles for Chondrocyte Regeneration: Schiff Base Self‐Assembly Approach |
| title_sort | self healing hyaluronic acid based hydrogel with mirna140 5p loaded mon pei nanoparticles for chondrocyte regeneration schiff base self assembly approach |
| topic | chondrocyte regeneration hydrogel miRNA140–5p self‐healing |
| url | https://doi.org/10.1002/advs.202406479 |
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