Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity
Background: <i>Annona muricata</i> L. (guanabana) leaves are rich in bioactive compounds with potential antioxidant properties. In the state of Colima, both ethanolic extracts and infusions are traditionally used in folk medicine to address various ailments. This study aimed to evaluate...
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2024-12-01
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| author | Gustavo A. Hernandez-Fuentes Osiris G. Delgado-Enciso Edgar G. Larios-Cedeño Juan M. Sánchez-Galindo Silvia G. Ceballos-Magaña Kayim Pineda-Urbina Mario A. Alcalá-Pérez Nancy E. Magaña-Vergara Josuel Delgado-Enciso Uriel Díaz-Llerenas Janet Diaz-Martinez Idalia Garza-Veloz Margarita L. Martinez-Fierro Iram P. Rodriguez-Sanchez Ivan Delgado-Enciso |
| author_facet | Gustavo A. Hernandez-Fuentes Osiris G. Delgado-Enciso Edgar G. Larios-Cedeño Juan M. Sánchez-Galindo Silvia G. Ceballos-Magaña Kayim Pineda-Urbina Mario A. Alcalá-Pérez Nancy E. Magaña-Vergara Josuel Delgado-Enciso Uriel Díaz-Llerenas Janet Diaz-Martinez Idalia Garza-Veloz Margarita L. Martinez-Fierro Iram P. Rodriguez-Sanchez Ivan Delgado-Enciso |
| author_sort | Gustavo A. Hernandez-Fuentes |
| collection | DOAJ |
| description | Background: <i>Annona muricata</i> L. (guanabana) leaves are rich in bioactive compounds with potential antioxidant properties. In the state of Colima, both ethanolic extracts and infusions are traditionally used in folk medicine to address various ailments. This study aimed to evaluate and compare the phytochemical composition and antioxidant activities of ethanolic extracts and infusions of <i>A. muricata</i> leaves from three geographic regions in Colima, Mexico, with a focus on how geographic origin affects their bioactive properties. Methods: Ethanolic extracts and infusions were prepared from <i>A. muricata</i> leaves and analyzed using phytochemical screening; DPPH, total antioxidant capacity (TAC), and total phenolic content (TPC) measurements; and HPLC. TLC was also conducted to examine the presence of specific compounds, such as flavonoids and phenols. Results: Both the ethanolic extracts and infusions contained significant levels of alkaloids, flavonoids, tannins, and phenolic compounds. The infusions demonstrated superior antioxidant capacity, with DPPH inhibition values of 72.5%, 68.3%, and 65.1% in the northern, central, and southern regions, respectively, compared to the ethanolic extracts’ values of 50.3%, 48.9%, and 45.0%. HPLC identified quercetin as a major compound across all samples. Geographically, the northern region exhibited higher concentrations of bioactive compounds, particularly total flavonoid content (TFC) and iron-reducing power (FRPA). Conclusions: Both the ethanolic extracts and infusions of <i>A. muricata</i> leaves exhibited significant antioxidant properties, with the infusions showing superior performance. The results suggest that <i>A. muricata</i> infusions may have potential applications in managing oxidative stress-related diseases such as cancer and diabetes. Exploring their use in traditional medicine and employing this type of approach can help discern the metabolite profile responsible for these bioactivities. Geographic factors influence the bioactive profile of the plant, and further research is needed to isolate specific bioactive compounds and elucidate their therapeutic mechanisms. |
| format | Article |
| id | doaj-art-e33dfbfa2b214b0d9a7feba00ce5bc0c |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-e33dfbfa2b214b0d9a7feba00ce5bc0c2024-12-27T14:36:21ZengMDPI AGLife2075-17292024-12-011412170210.3390/life14121702Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant ActivityGustavo A. Hernandez-Fuentes0Osiris G. Delgado-Enciso1Edgar G. Larios-Cedeño2Juan M. Sánchez-Galindo3Silvia G. Ceballos-Magaña4Kayim Pineda-Urbina5Mario A. Alcalá-Pérez6Nancy E. Magaña-Vergara7Josuel Delgado-Enciso8Uriel Díaz-Llerenas9Janet Diaz-Martinez10Idalia Garza-Veloz11Margarita L. Martinez-Fierro12Iram P. Rodriguez-Sanchez13Ivan Delgado-Enciso14Department of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, MexicoDepartment of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, MexicoState Cancerology Institute of Colima, Health Services of the Mexican Social Security Institute for Welfare (IMSS-BIENESTAR), Colima 28085, MexicoState Cancerology Institute of Colima, Health Services of the Mexican Social Security Institute for Welfare (IMSS-BIENESTAR), Colima 28085, MexicoFaculty of Sciences, University of Colima, Colima 28045, MexicoFaculty of Chemical Sciences, University of Colima, Coquimatlan 28400, MexicoMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, MexicoFaculty of Chemical Sciences, University of Colima, Coquimatlan 28400, MexicoFoundation for Ethics, Education, and Cancer Research of the State Cancer Institute of Colima AC, Colima 28085, MexicoMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, MexicoResearch Center in Minority Institutions, Robert Stempel College of Public Health, Florida International University (FIU-RCMI), Miami, FL 33199, USAMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, MexicoMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, MexicoMolecular and Structural Physiology Laboratory, School of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza 66455, MexicoDepartment of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, MexicoBackground: <i>Annona muricata</i> L. (guanabana) leaves are rich in bioactive compounds with potential antioxidant properties. In the state of Colima, both ethanolic extracts and infusions are traditionally used in folk medicine to address various ailments. This study aimed to evaluate and compare the phytochemical composition and antioxidant activities of ethanolic extracts and infusions of <i>A. muricata</i> leaves from three geographic regions in Colima, Mexico, with a focus on how geographic origin affects their bioactive properties. Methods: Ethanolic extracts and infusions were prepared from <i>A. muricata</i> leaves and analyzed using phytochemical screening; DPPH, total antioxidant capacity (TAC), and total phenolic content (TPC) measurements; and HPLC. TLC was also conducted to examine the presence of specific compounds, such as flavonoids and phenols. Results: Both the ethanolic extracts and infusions contained significant levels of alkaloids, flavonoids, tannins, and phenolic compounds. The infusions demonstrated superior antioxidant capacity, with DPPH inhibition values of 72.5%, 68.3%, and 65.1% in the northern, central, and southern regions, respectively, compared to the ethanolic extracts’ values of 50.3%, 48.9%, and 45.0%. HPLC identified quercetin as a major compound across all samples. Geographically, the northern region exhibited higher concentrations of bioactive compounds, particularly total flavonoid content (TFC) and iron-reducing power (FRPA). Conclusions: Both the ethanolic extracts and infusions of <i>A. muricata</i> leaves exhibited significant antioxidant properties, with the infusions showing superior performance. The results suggest that <i>A. muricata</i> infusions may have potential applications in managing oxidative stress-related diseases such as cancer and diabetes. Exploring their use in traditional medicine and employing this type of approach can help discern the metabolite profile responsible for these bioactivities. Geographic factors influence the bioactive profile of the plant, and further research is needed to isolate specific bioactive compounds and elucidate their therapeutic mechanisms.https://www.mdpi.com/2075-1729/14/12/1702<i>Annona muricata</i>traditional medicinephytochemistryantioxidant activityflavonoidspolyphenols |
| spellingShingle | Gustavo A. Hernandez-Fuentes Osiris G. Delgado-Enciso Edgar G. Larios-Cedeño Juan M. Sánchez-Galindo Silvia G. Ceballos-Magaña Kayim Pineda-Urbina Mario A. Alcalá-Pérez Nancy E. Magaña-Vergara Josuel Delgado-Enciso Uriel Díaz-Llerenas Janet Diaz-Martinez Idalia Garza-Veloz Margarita L. Martinez-Fierro Iram P. Rodriguez-Sanchez Ivan Delgado-Enciso Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity Life <i>Annona muricata</i> traditional medicine phytochemistry antioxidant activity flavonoids polyphenols |
| title | Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity |
| title_full | Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity |
| title_fullStr | Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity |
| title_full_unstemmed | Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity |
| title_short | Comparative Analysis of Infusions and Ethanolic Extracts of <i>Annona muricata</i> Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity |
| title_sort | comparative analysis of infusions and ethanolic extracts of i annona muricata i leaves from colima mexico phytochemical profile and antioxidant activity |
| topic | <i>Annona muricata</i> traditional medicine phytochemistry antioxidant activity flavonoids polyphenols |
| url | https://www.mdpi.com/2075-1729/14/12/1702 |
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