Protocol for tandem enrichment of ubiquitinated, phosphorylated, and glycosylated peptides with SCASP-PTM

Protocol for tandem enrichment of ubiquitinated, phosphorylated, and glycosylated peptides with SCASP-PTM

Summary: Here, we present a protocol for the tandem enrichment of ubiquitinated, phosphorylated, and glycosylated peptides from one sample using the SCASP-PTM (SDS-cyclodextrin-assisted sample preparation-post-translational modification) approach. We detail the steps for preparing samples for protei...

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Bibliographic Details
Main Authors: Zhan-Peng Lin, Yu-Yang Wang, Chengwen Wen, Kaijie Zhang, Xiangyu Chen, Xu Liang, Wan-Yue Luo, Changchuan Xie, Chuan-Qi Zhong
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cancer
Signal Transduction
Proteomics
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003508
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