In vivo assessment of endothelial function in small animals using an infrared pulse detector
Objective: Endothelial dysfunction is the earliest change in atherosclerosis. Flow-mediated dilatation (FMD) is used to assess endothelial function in humans. However, this assessment is not easy in small animals. This study demonstrated the reliability and reproducibility of a proposed instrument f...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Tzu Chi Medical Journal |
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| Online Access: | http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2019;volume=31;issue=4;spage=217;epage=221;aulast=Liu |
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| _version_ | 1849323351320821760 |
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| author | Cyuan-Cin Liu Wei-Min Liu Hsien-Tsai Wu Chien-Hsing Wang An-Bang Liu |
| author_facet | Cyuan-Cin Liu Wei-Min Liu Hsien-Tsai Wu Chien-Hsing Wang An-Bang Liu |
| author_sort | Cyuan-Cin Liu |
| collection | DOAJ |
| description | Objective: Endothelial dysfunction is the earliest change in atherosclerosis. Flow-mediated dilatation (FMD) is used to assess endothelial function in humans. However, this assessment is not easy in small animals. This study demonstrated the reliability and reproducibility of a proposed instrument for in vivo assessment of FMD in a rodent model using infrared pulse sensors. Materials and Methods: We used 24 adult male Wistar Kyoto rats randomly divided into three groups. FMD was measured under continuous infusion of normal saline followed by intra-arterial infusion of acetylcholine (Ach; n = 8), sodium nitroprusside (SNP; n = 8), or N∞-nitro-L-arginine methyl ester (L-NAME; n = 8). Results: The dilatation indices (DIs) of all three groups were similar before application of the vasoactive agents (1.82 ± 0.46, 1.81 ± 0.44, and 1.93 ± 0.40, P = 0.877, by one-way analysis of variance). The DI was significantly increased during infusion of Ach (2.97 ± 1.03 vs. 1.82 ± 0.46, P = 0.015), unchanged during infusion of SNP (1.81 ± 0.44 vs. 1.98 ± 0.40, P = 0.574), and attenuated during infusion of L-NAME (1.91 ± 0.40 vs. 1.42 ± 0.35; P = 0.028). Conclusion: The results of this study correlated well with those of human studies, suggesting that this method can be used for in vivo evaluation of endothelial function in small animals. |
| format | Article |
| id | doaj-art-e2bc1aa7fb774ed988cf36abc5b49b28 |
| institution | Kabale University |
| issn | 1016-3190 2223-8956 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Tzu Chi Medical Journal |
| spelling | doaj-art-e2bc1aa7fb774ed988cf36abc5b49b282025-08-20T03:49:03ZengWolters Kluwer Medknow PublicationsTzu Chi Medical Journal1016-31902223-89562019-01-0131421722110.4103/tcmj.tcmj_94_18In vivo assessment of endothelial function in small animals using an infrared pulse detectorCyuan-Cin LiuWei-Min LiuHsien-Tsai WuChien-Hsing WangAn-Bang LiuObjective: Endothelial dysfunction is the earliest change in atherosclerosis. Flow-mediated dilatation (FMD) is used to assess endothelial function in humans. However, this assessment is not easy in small animals. This study demonstrated the reliability and reproducibility of a proposed instrument for in vivo assessment of FMD in a rodent model using infrared pulse sensors. Materials and Methods: We used 24 adult male Wistar Kyoto rats randomly divided into three groups. FMD was measured under continuous infusion of normal saline followed by intra-arterial infusion of acetylcholine (Ach; n = 8), sodium nitroprusside (SNP; n = 8), or N∞-nitro-L-arginine methyl ester (L-NAME; n = 8). Results: The dilatation indices (DIs) of all three groups were similar before application of the vasoactive agents (1.82 ± 0.46, 1.81 ± 0.44, and 1.93 ± 0.40, P = 0.877, by one-way analysis of variance). The DI was significantly increased during infusion of Ach (2.97 ± 1.03 vs. 1.82 ± 0.46, P = 0.015), unchanged during infusion of SNP (1.81 ± 0.44 vs. 1.98 ± 0.40, P = 0.574), and attenuated during infusion of L-NAME (1.91 ± 0.40 vs. 1.42 ± 0.35; P = 0.028). Conclusion: The results of this study correlated well with those of human studies, suggesting that this method can be used for in vivo evaluation of endothelial function in small animals.http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2019;volume=31;issue=4;spage=217;epage=221;aulast=LiuEndothelial functionFlow-mediated dilatationNitric oxide |
| spellingShingle | Cyuan-Cin Liu Wei-Min Liu Hsien-Tsai Wu Chien-Hsing Wang An-Bang Liu In vivo assessment of endothelial function in small animals using an infrared pulse detector Tzu Chi Medical Journal Endothelial function Flow-mediated dilatation Nitric oxide |
| title | In vivo assessment of endothelial function in small animals using an infrared pulse detector |
| title_full | In vivo assessment of endothelial function in small animals using an infrared pulse detector |
| title_fullStr | In vivo assessment of endothelial function in small animals using an infrared pulse detector |
| title_full_unstemmed | In vivo assessment of endothelial function in small animals using an infrared pulse detector |
| title_short | In vivo assessment of endothelial function in small animals using an infrared pulse detector |
| title_sort | in vivo assessment of endothelial function in small animals using an infrared pulse detector |
| topic | Endothelial function Flow-mediated dilatation Nitric oxide |
| url | http://www.tcmjmed.com/article.asp?issn=1016-3190;year=2019;volume=31;issue=4;spage=217;epage=221;aulast=Liu |
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