Normalized Transillumination of Fluorescent Proteins in Small Animals
We investigated the capacity of normalized transillumination methods in imaging fluorescent proteins in visible light, in phantoms and in live mice. We demonstrate significant imaging improvements over epi-illumination imaging, as commonly applied today. Of particular importance is the significant b...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00018 |
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| _version_ | 1841564596505149440 |
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| author | Giannis Zacharakis Helen Shih Jorge Ripoll Ralph Weissleder Vasilis Ntziachristos |
| author_facet | Giannis Zacharakis Helen Shih Jorge Ripoll Ralph Weissleder Vasilis Ntziachristos |
| author_sort | Giannis Zacharakis |
| collection | DOAJ |
| description | We investigated the capacity of normalized transillumination methods in imaging fluorescent proteins in visible light, in phantoms and in live mice. We demonstrate significant imaging improvements over epi-illumination imaging, as commonly applied today. Of particular importance is the significant betterment in contrast achieved, due to minimization of the influence of autofluorescence and the enhancement of depth sensitivity compared to epi-illumination imaging. Although normalized transillumination does not match the accuracy and quantification capacity of tomographic imaging, it nevertheless attains key advantages over other planar optical imaging methods while preserving implementation simplicity. Due to the balance achieved between simplicity and accuracy, normalized transillumination approaches could serve as an important alternative molecular imaging method. |
| format | Article |
| id | doaj-art-e2a0cfe5e7f544ff9e19cd8241a5ccfd |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-e2a0cfe5e7f544ff9e19cd8241a5ccfd2025-01-02T22:37:22ZengSAGE PublishingMolecular Imaging1536-01212006-07-01510.2310/7290.2006.0001810.2310_7290.2006.00018Normalized Transillumination of Fluorescent Proteins in Small AnimalsGiannis Zacharakis0Helen Shih1Jorge Ripoll2Ralph Weissleder3Vasilis Ntziachristos4Foundation for Research and Technology—Hellas, GreeceHarvard Medical School, USAFoundation for Research and Technology—Hellas, GreeceHarvard Medical School, USAHarvard Medical School, USAWe investigated the capacity of normalized transillumination methods in imaging fluorescent proteins in visible light, in phantoms and in live mice. We demonstrate significant imaging improvements over epi-illumination imaging, as commonly applied today. Of particular importance is the significant betterment in contrast achieved, due to minimization of the influence of autofluorescence and the enhancement of depth sensitivity compared to epi-illumination imaging. Although normalized transillumination does not match the accuracy and quantification capacity of tomographic imaging, it nevertheless attains key advantages over other planar optical imaging methods while preserving implementation simplicity. Due to the balance achieved between simplicity and accuracy, normalized transillumination approaches could serve as an important alternative molecular imaging method.https://doi.org/10.2310/7290.2006.00018 |
| spellingShingle | Giannis Zacharakis Helen Shih Jorge Ripoll Ralph Weissleder Vasilis Ntziachristos Normalized Transillumination of Fluorescent Proteins in Small Animals Molecular Imaging |
| title | Normalized Transillumination of Fluorescent Proteins in Small Animals |
| title_full | Normalized Transillumination of Fluorescent Proteins in Small Animals |
| title_fullStr | Normalized Transillumination of Fluorescent Proteins in Small Animals |
| title_full_unstemmed | Normalized Transillumination of Fluorescent Proteins in Small Animals |
| title_short | Normalized Transillumination of Fluorescent Proteins in Small Animals |
| title_sort | normalized transillumination of fluorescent proteins in small animals |
| url | https://doi.org/10.2310/7290.2006.00018 |
| work_keys_str_mv | AT gianniszacharakis normalizedtransilluminationoffluorescentproteinsinsmallanimals AT helenshih normalizedtransilluminationoffluorescentproteinsinsmallanimals AT jorgeripoll normalizedtransilluminationoffluorescentproteinsinsmallanimals AT ralphweissleder normalizedtransilluminationoffluorescentproteinsinsmallanimals AT vasilisntziachristos normalizedtransilluminationoffluorescentproteinsinsmallanimals |