Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation
Huan Yang,1 Jun Cao,1 Lijie Zhou,1 Jiangchuan Chen,1 Jiaman Tang,1 Jiamei Chen,1 Lengyun Yin,1 Li Xie,2 Jianmin Li,1 Jinwen Luo3 1Department of Pulmonary and Critical Care Medicine, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Clinical Medicine Resear...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-01-01
|
| Series: | Drug Design, Development and Therapy |
| Subjects: | |
| Online Access: | https://www.dovepress.com/exploring-the-cardioprotective-mechanisms-of-ligusticum-wallichii-in-m-peer-reviewed-fulltext-article-DDDT |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526609332404224 |
|---|---|
| author | Yang H Cao J Zhou L Chen J Tang J Chen J Yin L Xie L Li J Luo J |
| author_facet | Yang H Cao J Zhou L Chen J Tang J Chen J Yin L Xie L Li J Luo J |
| author_sort | Yang H |
| collection | DOAJ |
| description | Huan Yang,1 Jun Cao,1 Lijie Zhou,1 Jiangchuan Chen,1 Jiaman Tang,1 Jiamei Chen,1 Lengyun Yin,1 Li Xie,2 Jianmin Li,1 Jinwen Luo3 1Department of Pulmonary and Critical Care Medicine, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Clinical Medicine Research Center For Respiratory Rehabilitation in Hunan Province, Changsha, Hunan, People’s Republic of China; 2Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China; 3Department of Cardio-Thoracic Surgery, Hunan Children’s Hospital, Changsha, Hunan, People’s Republic of ChinaCorrespondence: Jinwen Luo, Department of Cardio-thoracic Surgery, Hunan Children’s Hospital, Changsha, Hunan, People’s Republic of China, Email Jinwen_luo168@163.com Jianmin Li, Department of Pulmonary and Critical Care Medicine, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan, People’s Republic of China, Email Leo780307@sina.comBackground: Myocardial infarction represents a coronary artery ailment with the highest incidence and fatality rates among cardiovascular conditions. However, effective pharmacological interventions remain elusive. This study seeks to elucidate the molecular mechanisms underlying the effects of Ligusticum wallichii on myocardial infarction through network pharmacology and experimental validation.Methods: Initially, potential targets of Ligusticum wallichii’s active ingredients and myocardial infarction-related targets were retrieved from databases. Subsequently, core targets of Ligusticum wallichii on myocardial infarction were identified via the PPI network analysis and subjected to GO and KEGG pathway enrichment analyses. Molecular docking was employed to validate the binding affinities between the core targets and the bioactive components. The findings from network pharmacology analysis were corroborated through in vitro and in vivo experiments.Results: Seven active ingredients from Ligusticum wallichii were identified, corresponding to 122 targets. Molecular docking revealed robust binding affinities of Myricanone, Senkyunone, and Sitosterol to key target proteins (EGFR, STAT3, and SRC). In vitro, experiments demonstrated that pretreatment with the active components of Ligusticum wallichii protected myocardial cells from OGD exposure and modulated the expression of their key target genes. In vivo, experiments showed that the active components of Ligusticum wallichii significantly improved myocardial infarction via alleviating myocardial fibrosis and oxidative stress and did not elicit toxic effects in mice.Conclusion: The collective findings suggest that Ligusticum wallichii shows promising potential for myocardial infarction treatment by regulating key target proteins (EGFR, STAT3, and SRC), which play roles in oxidative stress and myocardial fibrosis.Keywords: Ligusticum wallichii, myocardial infarction, Molecular docking, network pharmacology, experimental validation |
| format | Article |
| id | doaj-art-e278b2fdea24446bb66aaf14338832b4 |
| institution | Kabale University |
| issn | 1177-8881 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj-art-e278b2fdea24446bb66aaf14338832b42025-01-16T16:17:13ZengDove Medical PressDrug Design, Development and Therapy1177-88812025-01-01Volume 1928130299324Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental ValidationYang HCao JZhou LChen JTang JChen JYin LXie LLi JLuo JHuan Yang,1 Jun Cao,1 Lijie Zhou,1 Jiangchuan Chen,1 Jiaman Tang,1 Jiamei Chen,1 Lengyun Yin,1 Li Xie,2 Jianmin Li,1 Jinwen Luo3 1Department of Pulmonary and Critical Care Medicine, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Clinical Medicine Research Center For Respiratory Rehabilitation in Hunan Province, Changsha, Hunan, People’s Republic of China; 2Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China; 3Department of Cardio-Thoracic Surgery, Hunan Children’s Hospital, Changsha, Hunan, People’s Republic of ChinaCorrespondence: Jinwen Luo, Department of Cardio-thoracic Surgery, Hunan Children’s Hospital, Changsha, Hunan, People’s Republic of China, Email Jinwen_luo168@163.com Jianmin Li, Department of Pulmonary and Critical Care Medicine, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan, People’s Republic of China, Email Leo780307@sina.comBackground: Myocardial infarction represents a coronary artery ailment with the highest incidence and fatality rates among cardiovascular conditions. However, effective pharmacological interventions remain elusive. This study seeks to elucidate the molecular mechanisms underlying the effects of Ligusticum wallichii on myocardial infarction through network pharmacology and experimental validation.Methods: Initially, potential targets of Ligusticum wallichii’s active ingredients and myocardial infarction-related targets were retrieved from databases. Subsequently, core targets of Ligusticum wallichii on myocardial infarction were identified via the PPI network analysis and subjected to GO and KEGG pathway enrichment analyses. Molecular docking was employed to validate the binding affinities between the core targets and the bioactive components. The findings from network pharmacology analysis were corroborated through in vitro and in vivo experiments.Results: Seven active ingredients from Ligusticum wallichii were identified, corresponding to 122 targets. Molecular docking revealed robust binding affinities of Myricanone, Senkyunone, and Sitosterol to key target proteins (EGFR, STAT3, and SRC). In vitro, experiments demonstrated that pretreatment with the active components of Ligusticum wallichii protected myocardial cells from OGD exposure and modulated the expression of their key target genes. In vivo, experiments showed that the active components of Ligusticum wallichii significantly improved myocardial infarction via alleviating myocardial fibrosis and oxidative stress and did not elicit toxic effects in mice.Conclusion: The collective findings suggest that Ligusticum wallichii shows promising potential for myocardial infarction treatment by regulating key target proteins (EGFR, STAT3, and SRC), which play roles in oxidative stress and myocardial fibrosis.Keywords: Ligusticum wallichii, myocardial infarction, Molecular docking, network pharmacology, experimental validationhttps://www.dovepress.com/exploring-the-cardioprotective-mechanisms-of-ligusticum-wallichii-in-m-peer-reviewed-fulltext-article-DDDTligusticum wallichiimyocardial infarctionmolecular dockingnetwork pharmacologyexperimental validation |
| spellingShingle | Yang H Cao J Zhou L Chen J Tang J Chen J Yin L Xie L Li J Luo J Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation Drug Design, Development and Therapy ligusticum wallichii myocardial infarction molecular docking network pharmacology experimental validation |
| title | Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation |
| title_full | Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation |
| title_fullStr | Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation |
| title_full_unstemmed | Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation |
| title_short | Exploring the Cardioprotective Mechanisms of Ligusticum wallichii in Myocardial Infarction Through Network Pharmacology and Experimental Validation |
| title_sort | exploring the cardioprotective mechanisms of ligusticum wallichii in myocardial infarction through network pharmacology and experimental validation |
| topic | ligusticum wallichii myocardial infarction molecular docking network pharmacology experimental validation |
| url | https://www.dovepress.com/exploring-the-cardioprotective-mechanisms-of-ligusticum-wallichii-in-m-peer-reviewed-fulltext-article-DDDT |
| work_keys_str_mv | AT yangh exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT caoj exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT zhoul exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT chenj exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT tangj exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT chenj exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT yinl exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT xiel exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT lij exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation AT luoj exploringthecardioprotectivemechanismsofligusticumwallichiiinmyocardialinfarctionthroughnetworkpharmacologyandexperimentalvalidation |