Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture
The process of tuber formation in potato depends on many factors: genotype, exogenous hormones, photoperiod, source and concentration of carbohydrate. This work aims to study microtuber formation in potato (Solanum tuberosum L.) cv. Nevskij cultured in vitro. The plants were cultivated on the Muras...
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| Language: | English |
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Kazan Federal University
2019-09-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2019-3-2.html |
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| author | A.T. Gizatullina Z. Stasevski E.A. Gimaeva G.F. Safiullina |
| author_facet | A.T. Gizatullina Z. Stasevski E.A. Gimaeva G.F. Safiullina |
| author_sort | A.T. Gizatullina |
| collection | DOAJ |
| description | The process of tuber formation in potato depends on many factors: genotype, exogenous hormones, photoperiod, source and concentration of carbohydrate. This work aims to study microtuber formation in potato (Solanum tuberosum L.) cv. Nevskij cultured in vitro.
The plants were cultivated on the Murashige and Skoog medium. We compared the effects of sucrose (30, 50, 60 and 80 g/L), kinetin (1, 1.75, and 2.5 mg/ L), and benzylaminopurine (BAP) (1, 3, 5, and 8 mg/L) in background sucrose 80 g/L under the conditions of short days (8/16 h, SD) and continuous darkness (0/24 h, CD). Potato explants planted on the experimental culture media were cultivated for the first 2 weeks under the long-day conditions (16/8 h, LD). At the next stage, the explants were cultivated within 10 weeks under the short-day SD and CD conditions. The growth and development of microtubers were observed. The morphometric parameters of the obtained microtubers by the end of the 12th week were evaluated. On the cultural medium containing 80 g/L sucrose, the number of microtubers larger than 5 mm increased under the conditions of SD from 20% to 56% compared with CD. Under the CD conditions, an increase in the yield of tubers larger than 5 mm, as compared with the hormone-free nutrient medium (80 g/L sucrose), was promoted by adding kinetin (1 and 2.5 mg/L) and BAP (5 mg/L) to the nutrient medium. |
| format | Article |
| id | doaj-art-e1a3018156074f35bf1c2e84c219f18f |
| institution | Kabale University |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2019-09-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-e1a3018156074f35bf1c2e84c219f18f2025-01-02T11:36:19ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2019-09-01161337538410.26907/2542-064X.2019.3.375-384Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro cultureA.T. Gizatullina0Z. Stasevski1E.A. Gimaeva2G.F. Safiullina3Tatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420059 RussiaTatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420059 RussiaTatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420059 RussiaTatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420059 RussiaThe process of tuber formation in potato depends on many factors: genotype, exogenous hormones, photoperiod, source and concentration of carbohydrate. This work aims to study microtuber formation in potato (Solanum tuberosum L.) cv. Nevskij cultured in vitro. The plants were cultivated on the Murashige and Skoog medium. We compared the effects of sucrose (30, 50, 60 and 80 g/L), kinetin (1, 1.75, and 2.5 mg/ L), and benzylaminopurine (BAP) (1, 3, 5, and 8 mg/L) in background sucrose 80 g/L under the conditions of short days (8/16 h, SD) and continuous darkness (0/24 h, CD). Potato explants planted on the experimental culture media were cultivated for the first 2 weeks under the long-day conditions (16/8 h, LD). At the next stage, the explants were cultivated within 10 weeks under the short-day SD and CD conditions. The growth and development of microtubers were observed. The morphometric parameters of the obtained microtubers by the end of the 12th week were evaluated. On the cultural medium containing 80 g/L sucrose, the number of microtubers larger than 5 mm increased under the conditions of SD from 20% to 56% compared with CD. Under the CD conditions, an increase in the yield of tubers larger than 5 mm, as compared with the hormone-free nutrient medium (80 g/L sucrose), was promoted by adding kinetin (1 and 2.5 mg/L) and BAP (5 mg/L) to the nutrient medium.https://kpfu.ru/uz-eng-ns-2019-3-2.htmlphytohormoneskinetinbenzoaminopurinesucrosemicrotuberscultural mediumphotoperiod |
| spellingShingle | A.T. Gizatullina Z. Stasevski E.A. Gimaeva G.F. Safiullina Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture Учёные записки Казанского университета: Серия Естественные науки phytohormones kinetin benzoaminopurine sucrose microtubers cultural medium photoperiod |
| title | Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture |
| title_full | Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture |
| title_fullStr | Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture |
| title_full_unstemmed | Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture |
| title_short | Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture |
| title_sort | potato solanum tuberosum l cv nevskij microtuber formation features in in vitro culture |
| topic | phytohormones kinetin benzoaminopurine sucrose microtubers cultural medium photoperiod |
| url | https://kpfu.ru/uz-eng-ns-2019-3-2.html |
| work_keys_str_mv | AT atgizatullina potatosolanumtuberosumlcvnevskijmicrotuberformationfeaturesininvitroculture AT zstasevski potatosolanumtuberosumlcvnevskijmicrotuberformationfeaturesininvitroculture AT eagimaeva potatosolanumtuberosumlcvnevskijmicrotuberformationfeaturesininvitroculture AT gfsafiullina potatosolanumtuberosumlcvnevskijmicrotuberformationfeaturesininvitroculture |